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(Plasmid #119997)


Item Catalog # Description Quantity Price (USD)
Plasmid 119997 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
    H. sapiens (human)
  • Promoter None
  • Tag / Fusion Protein
    • mKeima8.5 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer AAACCACAACTAGAATGCAGTG
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Template plasmid for C-terminal PCR tagging of mammalian genes. For M1 and M2 tagging oligo design please visit Please visit for BioRxiv preprint

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMaCTag-18 was a gift from Michael Knop (Addgene plasmid # 119997 ; ; RRID:Addgene_119997)
  • For your References section:

    CRISPR/Cas12a-assisted PCR tagging of mammalian genes. Fueller J, Meurer M, Herbst K, Gubicza K, Kurtulmus B, Knopf JD, Kirrmaier D, Buchmuller B, Pereira G, Lemberg MK, Knop M. bioRxiv 2018 10.1101/473876