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StrKDEL-IRES-ManII-mScarlet-i
(Plasmid #117274)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 117274 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 117274-D50 50 μg of DNA in Tris buffer $495
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DNA 117274-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pIRESneo3
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    partial sequence of mannosidase II
  • Species
    M. musculus (mouse)
  • Mutation
    contains only amino acids 1 to 116 of ManII
  • GenBank ID
    NM_008549.2
  • Entrez Gene
    Man2a1 (a.k.a. Mana-2, Mana2, Map-2)
  • Promoter IRES
  • Tag / Fusion Protein
    • mScarlet-i (C terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    Streptavidin-KDEL
  • Species
    Synthetic
  • Promoter CMV

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site See PDF (unknown if destroyed)
  • 3′ cloning site See PDF (unknown if destroyed)
  • 5′ sequencing primer CMV
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Original clone was Plasmid #65253 from Franck Perez. mScarlet-i was synthesized from published sequence.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    StrKDEL-IRES-ManII-mScarlet-i was a gift from David Stephens (Addgene plasmid # 117274 ; http://n2t.net/addgene:117274 ; RRID:Addgene_117274)

  • For your References section:

    ER-to-Golgi trafficking of procollagen in the absence of large carriers. McCaughey J, Stevenson NL, Cross S, Stephens DJ. J Cell Biol. 2018 Dec 26. pii: jcb.201806035. doi: 10.1083/jcb.201806035. 10.1083/jcb.201806035 PubMed 30587510
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