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pStr-KDEL-IRES-ST-mCherry
(Plasmid #110727)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 110727 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Str-KDEL
  • Species
    Synthetic
  • Promoter CMV
  • Tag / Fusion Protein
    • Str Streptavidin binding peptide (SBP) (C terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    STase sialyltransferase
  • Species
    H. sapiens (human)
  • Mutation
    contains only amino acids 1 to 43 of ST
  • Entrez Gene
    ST6GAL1 (a.k.a. SIAT1, ST6GalI, ST6N)
  • Promoter IRES
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information for Gene/Insert 2

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pStr-KDEL-IRES-ST-mCherry was a gift from David Stephens (Addgene plasmid # 110727)
  • For your References section:

    ER-to-Golgi trafficking of procollagen in the absence of large carriers. McCaughey J, Stevenson NL, Cross S, Stephens DJ. J Cell Biol. 2018 Dec 26. pii: jcb.201806035. doi: 10.1083/jcb.201806035. 10.1083/jcb.201806035 PubMed 30587510