StrKDEL-IRES_mScarleti_Sec23A
(Plasmid #117273)

• Purpose: Expresses an Str-KDEL Hook for the RUSH system and separately mScarleti-Sec23A
• Depositing Lab: David Stephens
• Publication: McCaughey et al J Cell Biol. 2018 Dec 26. pii: jcb.201806035. doi: 10.1083/jcb.201806035.

Backbone

• Vector backbone: pIRES_neo3
• Backbone manufacturer: Clontech
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: SEC23A
• Alt name: Sec23 homolog A, coat complex II component
• Alt name: hSec23A
• Species: H. sapiens (human)
• Mutation: Human Sec23A; Sequencing showed a mutation in Sec23A in position 631 fom C -> G, resulting in an amino acid change from Leu -> Val.
• GenBank ID: NM_006364.3
• Entrez Gene: SEC23A (a.k.a. CLSD, hSec23A)
• Promoter: IRES
• Tag / Fusion Protein:
  • mScarlet-i (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: See PDF (unknown if destroyed)
• 3′ cloning site: See PDF (unknown if destroyed)
• 5′ sequencing primer: CMV

Gene/Insert 2

• Gene/Insert name: Streptavidin-KDEL
• Species: Synthetic
• Promoter: CMV

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: See PDF (unknown if destroyed)
• 3′ cloning site: See PDF (unknown if destroyed)
• 5′ sequencing primer: CMV

Resource Information

• Supplemental Documents:
  • Cloning information
• A portion of this plasmid was derived from a plasmid made by: Original clone was Plasmid #65253 from Franck Perez. mScarlet-i was synthesized from published sequence.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry