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StrKDEL-IRES_mScarleti_Sec23A
(Plasmid #117273)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 117273 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    SEC23A
  • Alt name
    Sec23 homolog A, coat complex II component
  • Alt name
    hSec23A
  • Species
    H. sapiens (human)
  • Mutation
    Human Sec23A; Sequencing showed a mutation in Sec23A in position 631 fom C -> G, resulting in an amino acid change from Leu -> Val.
  • GenBank ID
    NM_006364.3
  • Promoter IRES
  • Tag / Fusion Protein
    • mScarlet-i (C terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site See PDF (unknown if destroyed)
  • 3′ cloning site See PDF (unknown if destroyed)
  • 5′ sequencing primer CMV
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Streptavidin-KDEL
  • Species
    Synthetic
  • Promoter CMV

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site See PDF (unknown if destroyed)
  • 3′ cloning site See PDF (unknown if destroyed)
  • 5′ sequencing primer CMV
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Original clone was Plasmid #65253 from Franck Perez. mScarlet-i was synthesized from published sequence.
  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    StrKDEL-IRES_mScarleti_Sec23A was a gift from David Stephens (Addgene plasmid # 117273 ; http://n2t.net/addgene:117273 ; RRID:Addgene_117273)
  • For your References section:

    ER-to-Golgi trafficking of procollagen in the absence of large carriers. McCaughey J, Stevenson NL, Cross S, Stephens DJ. J Cell Biol. 2018 Dec 26. pii: jcb.201806035. doi: 10.1083/jcb.201806035. 10.1083/jcb.201806035 PubMed 30587510