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pDONR221-ARF5

Plasmid

#67312

Purpose

hArf5 with stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF5 (ARF5 Human)

Use

Gateway Cloning

Expression

Bacterial

Mutation

bp 477 T to C; silent mutation

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pGTM_SUMO_PLpro_W106F_001

Plasmid

#234491

Purpose

Expresses SARS-CoV-2 Nsp3 residues 746-1063 for the purification of PLpro protease with a W106F mutation to allow for 5-fluorotryptophan incorporation and 19F NMR

Depositor

Gaetano Montelione

Article

De Falco et al STAR Protoc. 2025 Sep 19;6(3):103952. do

Insert

PLpro (ORF1ab SARS-CoV-2)

Tags

6xHis-Sumo

Expression

Bacterial

Promoter

T7 ac

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pYPQ132B-TLS-CsALSgR1.1

Plasmid

#245877

Purpose

Golden gate entry vector carrying the 1st gRNA for base editing in Carrizo citrange ALS gene along with TLS mobile RNA sequence

Depositor

Yiping Qi

Article

Rocha et al Plant Cell Rep. 2025 Oct 22;44(11):244.

Insert

CsALSgR1.1

Use

CRISPR

Expression

Plant

Promoter

AtU3

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pYPQ132B-TLS-PtALSgRNA

Plasmid

#245881

Purpose

Golden gate entry vector carrying the gRNA for base editing in Poplar hybrid ALS gene along with TLS mobile RNA sequence

Depositor

Yiping Qi

Article

Rocha et al Plant Cell Rep. 2025 Oct 22;44(11):244.

Insert

PtALSgRNA

Use

CRISPR

Expression

Plant

Promoter

AtU3

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pEN_TT PCSK5(sgRR,M452I)-V5

Plasmid

#232450

Purpose

Gateway entry vector for an inducible sg09 resistant PCSK5_M452I mutant

Depositor

Kevin Janes

Article

Marohl et al Mol Cancer Res. 2025 Oct 28. doi: 10.115

Insert

PCSK5(M452I) (PCSK5 Human)

Use

Gateway Cloning

Tags

V5

Mutation

a75g_t84c_g87c; M452I

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pEN_TT PCSK5(sgRR,T288P)-V5

Plasmid

#232451

Purpose

Gateway entry vector for an inducible sg09 resistant PCSK5_T288P mutant

Depositor

Kevin Janes

Article

Marohl et al Mol Cancer Res. 2025 Oct 28. doi: 10.115

Insert

PCSK5(T288P) (PCSK5 Human)

Use

Gateway Cloning

Tags

V5

Mutation

a75g_t84c_g87c; T288P

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pDonr201 RNF146 deltaWWE

Plasmid

#240331

Purpose

Entry vector for Gateway with RNF146 (WWE domain deletion)

Depositor

Junjie Chen

Article

Nie et al Mol Cell Proteomics. 2020 Dec;19(12):201

Insert

RNF146 (RNF146 Human)

Expression

Bacterial

Mutation

WWE domain deletion

Available Since

Nov. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pDonr201 RNF146 deltaRING

Plasmid

#240332

Purpose

Entry vector for Gateway with RNF146 (RING domain deletion)

Depositor

Junjie Chen

Article

Nie et al Mol Cell Proteomics. 2020 Dec;19(12):201

Insert

RNF146 (RNF146 Human)

Expression

Bacterial

Mutation

RING domain deletion

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pDonr 201_MGA

Plasmid

#240327

Purpose

Entry vector for Gateway with MGA

Depositor

Junjie Chen

Article

Nie et al Genes Dev. 2022 Sep 1;36(17-18):1016-103

Insert

MGA (MGA Human)

Expression

Bacterial

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR223-ABL2(WT)

Plasmid

#215467

Purpose

Gateway entry vector with Arg wild-type (WT)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

ABL2 (ABL2 Human)

Use

Gateway Cloning

Mutation

Includes stop codon

Promoter

none

Available Since

Sept. 3, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR_HA-spGFP11

Plasmid

#240224

Purpose

Gateway entry clone for cloning in insert sequences by gibson assembly to create a C-terminal HA-spGFP11 tag. No ATG start codon.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al Nat Commun. 2026 Apr 20. doi: 10.1038/s4

Type

Empty backbone

Use

Gateway shuttle vector

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR221-Illusia(YYEE)

Plasmid

#222591

Purpose

Gateway entry vector with a phospho-mimetic mutant (YYEE) of the Illusia FRET reporter for integrin phosphorylation

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

Illusia(YYEE)

Use

Gateway Cloning

Mutation

Mutations in the NPxY sites equivolent to Y783E, …

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR221-Illusia(Y783F)

Plasmid

#222595

Purpose

Gateway entry vector with a phospho-mutant (Y783F) variant of the Illusia FRET reporter for integrin phosphorylation

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

Illusia(Y783F)

Use

Gateway Cloning

Mutation

Mutation in the NPxY(783) site equivolent to Y783…

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR221-Illusia(Y795F)

Plasmid

#222597

Purpose

Gateway entry vector with a phospho-mutant (Y795F) variant of the Illusia FRET reporter for integrin phosphorylation

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

Illusia(Y795F)

Use

Gateway Cloning

Mutation

Mutation in the NPxY(795) site equivolent to Y795…

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR2b-PTPN12(D199A,C231S)

Plasmid

#215516

Purpose

Gateway entry vector with a phosphatase-inactive PTP-PEST mutant (D199A,C231S)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PTPN12 (PTPN12 Human)

Use

Gateway Cloning

Mutation

D199A, C231S (Phosphatase inactive)

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR2b-Dok1-Clover

Plasmid

#215455

Purpose

Gateway entry vector with Dok1 tagged with Clover

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

DOK1 (DOK1 Human)

Use

Gateway Cloning

Tags

Clover

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR221-Illusia(YYFF)

Plasmid

#215443

Purpose

Gateway entry vector with inactive phospho-mutant (YYFF) of Illusia FRET reporter for integrin phosphorylation

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

Illusia(YYFF)

Use

Gateway Cloning

Mutation

Mutations in the NPxY sites equivolent to Y783F, …

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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JDW 1391 (pME-V5-FOXF1-P2A-H2A-mCherry)

Plasmid

#232132

Purpose

A middle entry gateway clone containing V5 tagged human FOXF1 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

FOXF1 (FOXF1 Human)

Use

Gateway Cloning

Tags

V5

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pGG-PIP44

Plasmid

#196782

Purpose

Contains WRKY70 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

WRKY DNA-BINDING PROTEIN 70 (WRKY70 Mustard Weed)

Use

Greengate (gg) entry vector

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGG-PIP50

Plasmid

#196788

Purpose

Contains ACS2 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

1-AMINO-CYCLOPROPANE-1-CARBOXYLATE SYNTHASE 2 (ACS2 Mustard Weed)

Use

Greengate (gg) entry vector

Mutation

150 C->G

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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