We narrowed to 17,340 results for: emb

1. pMB1610_pRR-Puro

   Plasmid

   #65853

   Purpose

   Puromycin-based homologous recombination-dependent reporter for genome editing with TALENs or CRISPR/Cas9

   Depositor

   Marc Bühler

   Article

   Flemr et al Cell Rep. 2015 Jul 28;12(4):709-16. doi:

   Insert

   split puromycin N-acetyltransferase coding sequence
   

   Use

   CRISPR and TALEN

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   Aug. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGEX6p-1-FRB-PafA

   Plasmid

   #113404

   Purpose

   Expresses FRB-PafA in bacteria

   Depositor

   Min Zhuang

   Article

   Liu et al Nat Methods. 2018 Aug 13. pii: 10.1038/s

   Insert

   FRB-PafA
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Aug. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. qTAG-N-Blast-moxGFP

   Plasmid

   #207677

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-moxGFP cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. hRIP1 HA GFP wt

   Plasmid

   #41396

   Depositor

   Francis Chan

   Article

   Cho et al Cell. 2009 Jun 12;137(6):1112-23.

   Insert

   RIP1 (RIPK1 Human)
   

   Tags

   GFP and HA

   Expression

   Mammalian

   Mutation

   E96G

   Promoter

   CMV

   Available Since

   Dec. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. rArc

   Plasmid

   #178206

   Purpose

   Ratiometric version of the genetically encoded voltage indicator ArcLight-Q239.

   Depositor

   Stefan Heinemann

   Article

   Ruhl et al Commun Biol. 2021 Oct 7;4(1):1164. doi:

   Insert

   rArc
   

   Expression

   Mammalian

   Available Since

   Feb. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. iRFP670-PIP5K1A

   Plasmid

   #202749

   Purpose

   expresses fluorescent protein-tagged enzyme

   Depositor

   Gerry Hammond

   Article

   Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

   Insert

   iRFP670:PIP5K1A (PIP5K1A Human)
   

   Expression

   Mammalian

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. C413-E38: attB4-T7p>-attB1r

   Plasmid

   #162929

   Purpose

   Gateway attB4/attB1r entry clone for bacterial expression using the bacteriophage T7 promoter

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   bacteriophage T7 promoter
   

   Use

   Synthetic Biology

   Available Since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. qTAG-N-Puro-miRFP670nano3

   Plasmid

   #207691

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-miRFP670nano3 cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pFUS_B4

   Plasmid

   #31021

   Depositor

   Adam Bogdanove, Daniel Voytas

   Article

   Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

   Insert

   LacZ + BsaI restriction sites
   

   Available Since

   June 30, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. hRIP3 GFP RHIM mut

    Plasmid

    #41385

    Depositor

    Francis Chan

    Article

    Cho et al Cell. 2009 Jun 12;137(6):1112-23.

    Insert

    RIP3 (RIPK3 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    RHIM mutant (AAAA-459-462)

    Promoter

    CMV

    Available Since

    Dec. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pMP117

    Plasmid

    #207920

    Purpose

    GreenBraid destination vector pGBZ2 - PaqCI - Eco31I - spisPink- Eco31I - PaqCI

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Nov. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pETNb-nALFA

    Plasmid

    #159988

    Purpose

    For the protein expression with ALFA tag at N-terminus in E. coli

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Type

    Empty backbone

    Tags

    MVKYI-8histidine-ALFA tag-HRV3C protease cleavage…

    Expression

    Bacterial

    Available Since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBTSG

    Plasmid

    #159420

    Purpose

    Transient expression of proteins with a C-terminal thermostable GFP (TGP) tag in mammalian cells

    Depositor

    Dianfan Li

    Article

    Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

    Insert

    thermostable GFP (TGP)
    

    Tags

    polyhistidine (poly-His)

    Expression

    Mammalian

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pETSG

    Plasmid

    #159418

    Purpose

    Expression of proteins with a C-terminal thermostable GFP (TGP) tag in Escherichia coli

    Depositor

    Dianfan Li

    Article

    Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

    Insert

    thermostable GFP (TGP)
    

    Tags

    polyhistidine (poly-His)

    Expression

    Bacterial

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMaCTag-13

    Plasmid

    #119992

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (without selection) with mCherry

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mCherry
    

    Use

    Pcr template

    Tags

    mCherry

    Promoter

    None

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. P16ΔS:S10-(MCS)

    Plasmid

    #108182

    Purpose

    Binary vectors used for the constitutive expression of tripartite split-sfGFP components in plants

    Depositor

    Tzu-Yin Liu

    Article

    Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Plant

    Promoter

    P16ΔS

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. R4227X-F

    Plasmid

    #21371

    Depositor

    Gregory Germino

    Article

    Hanaoka et al Nature. . 408(6815):990-4.

    Insert

    autosomal dominant polycystic kidney disease type I (PKD1 Human)
    

    Tags

    FLAG (following D4226)

    Expression

    Mammalian

    Mutation

    Changed R4227 to stop codon

    Available Since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

18. p3XFLAG-CMV 7.1_syn6

    Plasmid

    #50012

    Purpose

    Encodes syntaxin 6 with a N-terminal 3X FLAG tag

    Depositor

    David Hackstadt

    Article

    Moore et al Microbiology. 2011 Mar;157(Pt 3):830-8.

    Insert

    syntaxin 6 (Stx6 Rat)
    

    Tags

    3x FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMXs-Dnmt3l

    Plasmid

    #13356

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

    Insert

    DNA (cytosine-5-)-methyltransferase 3-like (Dnmt3l Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

20. zbFGF

    Plasmid

    #12309

    Depositor

    James Thomson

    Article

    Ludwig et al Nat Methods. 2006 Aug . 3(8):637-646.

    Insert

    Fgf2 (fgf2 Zebrafish)
    

    Expression

    Bacterial

    Mutation

    Ala 11 mutated to Pro, Ser 55 mutated to Asn

    Available Since

    Sept. 25, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge