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Gi-c010

Plasmid

#173080

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

GAP

Tags

mCitrine

Expression

Bacterial

Mutation

mCitrine + last 10 residues of GNA1, codon optimi…

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pRO117

Plasmid

#233823

Purpose

MoClo-YTK; type3; Reporter; mNeonGreen

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

mNeonGreen

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pUC-UAS1B8-Leum

Plasmid

#44316

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B8-Leum promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B8-Leum

Available Since

April 18, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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p3HM2Luc

Plasmid

#234807

Purpose

Mammalian expression of human muscarinic M2 receptor fused to RLuc8

Depositor

Vsevolod Gurevich

Article

Gimenez et al J Biol Chem. 2012 Aug 24;287(35):29495-5

Insert

Muscarinic M2 receptor

Tags

3xHA and RLuc8

Expression

Mammalian

Mutation

none

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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p3HB2ALuc

Plasmid

#234808

Purpose

Mammalian expression of human B2-adrenergic receptor fused to RLuc8

Depositor

Vsevolod Gurevich

Article

Gimenez et al J Biol Chem. 2012 Aug 24;287(35):29495-5

Insert

B2-adrenergic receptor

Tags

3xHA and RLuc8

Expression

Mammalian

Mutation

none

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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hSDH5

Plasmid

#113046

Purpose

Expression of WT hSDH5

Depositor

Edward A. Berry

Article

Zafreen et al Arch Biochem Biophys. 2016 Aug 15;604:47

Insert

hSDH5 (SDHAF2 Human)

Expression

Bacterial

Mutation

Insert begins at amino acid 39

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD_IPF2.0

Plasmid

#163124

Purpose

A plasmid with IFP2.0 coding sequence coded into it. Expresses IFP2.0 with N-term His-tag.

Depositor

Julie Biteen

Article

Chia et al Curr Res Microb Sci. 2020 Sep;1:1-6. doi

Insert

IFP2.0

Tags

His

Expression

Bacterial

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pUC-UAS1B8-TEF(136)

Plasmid

#44380

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B8-TEF(136) promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B8-TEF(136)

Available Since

April 17, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pUC-UAS1B16-TEF(272)

Plasmid

#44375

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B16-TEF(272) promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B16-TEF(272)

Available Since

April 18, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pEQ1180

Plasmid

#83805

Purpose

express human cytomegalovirus TRS1 with a C-terminal 6X his tag in eukaryotic cells

Depositor

Adam Geballe

Article

Child et al J Virol. 2012 Apr;86(7):3880-9. doi: 10.

Insert

TRS1

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only
pFCK(1.3)GW

Plasmid

#27230

Depositor

Pavel Osten

Article

Dittgen et al Proc Natl Acad Sci U S A. 2004 Dec 28. 1

Insert

EGFP

Use

Lentiviral

Tags

EGFP

Available Since

March 11, 2011

Availability

Academic Institutions and Nonprofits only

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pCAG-postASAP-p2a-ChrimsonR

Plasmid

#178794

Purpose

Expresses voltage sensor targeted to dendrites and spines along with ChrimsonR

Depositor

Rafael Yuste

Article

Cornejo et al Science. 2022 Jan 7;375(6576):82-86. doi

Insert

FingR.PSD95, ASAP2f and ChrimsonR

Tags

mRuby2

Expression

Mammalian

Mutation

mutations in amino acids L146G, S147T N149R, S150…

Promoter

CAG

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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YIplac211-Sec31-yoHalo

Plasmid

#115423

Purpose

Express Sec31-yoHalo at the endogenous locus via pop-in pop-out mutagenesis

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

Insert

Sec31-yoHalo

Tags

yoHaloTag

Expression

Yeast

Promoter

Endogenous

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pOPINK-UBE2S

Plasmid

#66714

Purpose

Expresses UBE2S (1-196) in E. coli

Depositor

David Komander

Article

Bremm et al Nat Struct Mol Biol. 2010 Aug;17(8):939-

Insert

e2-epf (UBE2S )

Tags

GST-3C

Expression

Bacterial

Promoter

T7

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only
pUC-UAS1B8-TEF(272)

Plasmid

#44376

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B8-TEF(272) promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B8-TEF(272)

Available Since

April 16, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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JAB2240

Plasmid

#190359

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::PhlF-NLS-19Sterm)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proG1090::PhlF-NLS-19Sterm

Expression

Plant

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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hSDH5_G78R

Plasmid

#113047

Purpose

Expression of mutant hSDH5

Depositor

Edward A. Berry

Article

Zafreen et al Arch Biochem Biophys. 2016 Aug 15;604:47

Insert

hSDH5_G78R (SDHAF2 Human)

Expression

Bacterial

Mutation

G78R, Insert begins at amino acid 39

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pUb6-Zpo1-V5/His

Plasmid

#29323

Depositor

Zena Werb

Article

Slorach et al Genes Dev. 2011 Mar 1;25(5):471-84. doi:

Insert

Zeppo1

Tags

His and V5

Expression

Mammalian

Available Since

May 5, 2011

Availability

Academic Institutions and Nonprofits only
hSDH5_C83D

Plasmid

#113048

Purpose

Expression of mutant hSDH5

Depositor

Edward A. Berry

Article

Zafreen et al Arch Biochem Biophys. 2016 Aug 15;604:47

Insert

hSDH5_C83D (SDHAF2 Human)

Expression

Bacterial

Mutation

C83D, Insert begins at amino acid 39

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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Human NCKX4-AS-long (pcDNA3.1+)

Plasmid

#75211

Purpose

Mammalian expression of SLC24A4

Depositor

Jonathan Lytton

Article

Li et al J Biol Chem. 2002 Dec 13;277(50):48410-7

Insert

SLC24A4 (SLC24A4 Human)

Expression

Mammalian

Mutation

alternatively spliced exon present

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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