We narrowed to 14,937 results for: pET

1. pJMP8932

   Plasmid

   #220906

   Purpose

   MobileCRiSPRi system encoded on a kanR Tn7 transposon; PD-sgRNA (BsaI site) and PG-dCas9-novo (for cloning new guides)

   Depositor

   Jason Peters

   Article

   Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

   Insert

   dCas9-novo
   

   Use

   CRISPR

   Tags

   3xMyc

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. CpX-1

   Plasmid

   #218676

   Purpose

   Expresses complexin-1 in bacteria

   Depositor

   Axel Brunger

   Article

   Leitz et al Nat Protoc. 2024 Jul 2. doi: 10.1038/s41

   Insert

   Rat complexin-1 (Cplx1 Rat)
   

   Use

   Tags

   6 his

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMMP_lambda

   Plasmid

   #217829

   Purpose

   Mini-mutagenesis plasmid for lambda light chain YSD Fabs

   Depositor

   Timothy Whitehead

   Article

   Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

   Type

   Empty backbone

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMMP_kappa

   Plasmid

   #217828

   Purpose

   Mini-mutagenesis plasmid for kappa light chain YSD Fabs

   Depositor

   Timothy Whitehead

   Article

   Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

   Type

   Empty backbone

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pnEA-NpM-Dm4E-T_9-44-F41DW42D-GB1_AA

   Plasmid

   #148256

   Purpose

   Bacterial Expression of Dm4E-T_9-44-F41DW42D

   Depositor

   Elisa Izaurralde

   Article

   Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

   Insert

   Dm4E-T_9-44-F41DW42D (4E-T Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pnEA-NpM-Dm4E-T_9-44-K12E-GB1_AA

   Plasmid

   #148257

   Purpose

   Bacterial Expression of Dm4E-T_9-44-K12E

   Depositor

   Elisa Izaurralde

   Article

   Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

   Insert

   Dm4E-T_9-44-K12E (4E-T Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pnEA-NpM-Dm4E-T_9-44-K12ER19E-GB1_AA

   Plasmid

   #148258

   Purpose

   Bacterial Expression of Dm4E-T_9-44-K12ER19E

   Depositor

   Elisa Izaurralde

   Article

   Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

   Insert

   Dm4E-T_9-44-K12ER19E (4E-T Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pnEA-NpM-Dm4E-T_9-44-K12ER19EK43E-GB1_AA

   Plasmid

   #148259

   Purpose

   Bacterial Expression of Dm4E-T_9-44-K12ER19EK43E

   Depositor

   Elisa Izaurralde

   Article

   Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

   Insert

   Dm4E-T_9-44-K12ER19EK43E (4E-T Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pnEA-NpM-Dm4E-T_9-44-R19E-GB1_AA

   Plasmid

   #148260

   Purpose

   Bacterial Expression of Dm4E-T_9-44-R19E

   Depositor

   Elisa Izaurralde

   Article

   Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

   Insert

   Dm4E-T_9-44-R19E (4E-T Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pnEA-NpM-Dm4E-T_9-44-YLFWtoAADD-GB1_AA

    Plasmid

    #148261

    Purpose

    Bacterial Expression of Dm4E-T_9-44-YLFWtoAADD

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

    Insert

    Dm4E-T_9-44-YLFWtoAADD (4E-T Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEM_HcBOP1ainsitu

    Plasmid

    #136529

    Purpose

    Template for generating in situ hybridization probe

    Depositor

    Junko Kyozuka

    Article

    Toriba et al Curr Biol. 2020 Feb 3;30(3):509-516.e3.

    Insert

    Houttuynia cordata BLADE-ON-PETIOLE homolog1a
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEM_ZmaBOP1binsitu

    Plasmid

    #136531

    Purpose

    Template for generating in situ hybridization probe

    Depositor

    Junko Kyozuka

    Article

    Toriba et al Curr Biol. 2020 Feb 3;30(3):509-516.e3.

    Insert

    Zoysia matrella BLADE-ON-PETIOLE homolog1b
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEM_ZmaBOP2binsitu

    Plasmid

    #136532

    Purpose

    Template for generating in situ hybridization probe

    Depositor

    Junko Kyozuka

    Article

    Toriba et al Curr Biol. 2020 Feb 3;30(3):509-516.e3.

    Insert

    Zoysia matrella BLADE-ON-PETIOLE homolog2b
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEM_HcBOP1binsitu

    Plasmid

    #136530

    Purpose

    Template for generating in situ hybridization probe

    Depositor

    Junko Kyozuka

    Article

    Toriba et al Curr Biol. 2020 Feb 3;30(3):509-516.e3.

    Insert

    Houttuynia cordata BLADE-ON-PETIOLE homolog1b
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOCC111

    Plasmid

    #118906

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal sortase site (LPETGg) and HIS6

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-NotI-ccdB-AscI-LPETGg-HIS6-stop-HindIII cassette
    

    Use

    Tags

    LPETGg, HIS6

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHL 802

    Plasmid

    #52991

    Purpose

    pLtetO-1:OxyS-mCherry, pLlacO-1:ompC::gfp

    Depositor

    Han Lim

    Article

    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

    Inserts

    OxyS sRNA
    ompC
    

    Use

    Synthetic Biology

    Tags

    GFP and RBS-mCherry

    Expression

    Bacterial

    Mutation

    contains −81 to +36 bp relative to start codon

    Promoter

    pLlacO-1 and pLtetO-1

    Available since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pCMV Ub Lex VP16 (F442A) HA (P#1711)

    Plasmid

    #14596

    Depositor

    Matija Peterlin

    Article

    Kurosu et al Curr Biol. 2004 Jun 22. 14(12):1112-6.

    Insert

    LexA
    

    Use

    Tags

    HA, Ubiquitin, and VP16 (F442A)

    Expression

    Mammalian

    Mutation

    The VP16 sequence contains an F442A mutation. The…

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pEF myc Gal cycT2b (1-420) (P#1720)

    Plasmid

    #14602

    Depositor

    Matija Peterlin

    Article

    Kurosu et al Gene. 2004 Dec 8. 343(1):173-9.

    Insert

    cycT2 (CCNT2 Human)
    

    Use

    Tags

    Gal and myc

    Expression

    Mammalian

    Mutation

    amino acids 1-420 of cyclinT2

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pGEX cycT2b (1-589) (P#1732)

    Plasmid

    #14604

    Depositor

    Matija Peterlin

    Article

    Kurosu et al Gene. 2004 Dec 8. 343(1):173-9.

    Insert

    cycT2 (1-589) (CCNT2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    amino acids 1-589

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

20. CapChR2_pmScarlet-N1

    Plasmid

    #188032

    Purpose

    Expresses CapChR2-mScarlet in mammalian cells

    Depositor

    Peter Hegemann

    Article

    Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

    Insert

    CapChR2-mScarlet
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    S43D, L112C, T139C and N238E mutations in CoChR

    Promoter

    CMV

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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