We narrowed to 32,755 results for: eng

1. pGEM-cytoBirA-2A-mCherry-SV40pA-FKF

   Plasmid

   #79887

   Purpose

   Donor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   HA-tagged BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
   

   Expression

   Bacterial

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMT-myl7-cytoBirA-2A-mCherry_Ras

   Plasmid

   #80060

   Purpose

   Myl7 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Use

   Zebrafish biotagging

   Tags

   3x HA

   Expression

   Bacterial

   Promoter

   Myl7 promoter

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TMED3-hLOV-TEVcs-GAL4bd-VP64-V5

   Plasmid

   #170996

   Purpose

   HiLITR transcription factor with full-length TMED3 targeting sequence (ER/Golgi)

   Depositor

   Alice Ting

   Article

   Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

   Insert

   TMED3(FL)-NNES-hLOV-TEVcs(ENLYFQ/M)-GAL4bd-VP64-V5 (TMED3 Synthetic)
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   V5

   Expression

   Mammalian

   Promoter

   Ef1-alpha

   Available Since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.3-hALDOA D34S

   Plasmid

   #210810

   Purpose

   To reduce the catalytic constant (kcat) for conversion of FBP to dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P)

   Depositor

   Sheng-cai Lin

   Article

   Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

   Insert

   ALDOA (ALDOA Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   residues 34 of ALDOA mutated to serine

   Promoter

   CMV

   Available Since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. BE3-hA3A-Y130F

   Plasmid

   #132945

   Purpose

   Gene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.

   Depositor

   Hui Yang

   Article

   Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:

   Insert

   BE3-hA3A(Y130F)
   

   Use

   CRISPR; Base editor

   Expression

   Mammalian

   Mutation

   BE3-hA3A(Y130F)

   Available Since

   Nov. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLV V5-TbID-emerin

   Plasmid

   #175099

   Purpose

   Lentiviral expression of V5-TbID-tagged mouse Emd

   Depositor

   Larry Gerace

   Article

   Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

   Insert

   Emerin (Emd Mouse)
   

   Use

   Lentiviral

   Tags

   V5-TbID

   Expression

   Mammalian

   Available Since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMT-zic2a-cytoBirA-2A-mCherry_Ras

   Plasmid

   #80064

   Purpose

   Zic2a promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Use

   Zebrafish biotagging

   Tags

   3x HA

   Expression

   Bacterial

   Promoter

   Zic2a enhancer driving c-fos promoter

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMT-ubb-NLS-BirA-2a-mCherry

   Plasmid

   #79886

   Purpose

   Ubiquitin promoter driving HA-tagged biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Tags

   3x HA

   Expression

   Bacterial

   Promoter

   Ubiquitin

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMT-MCS-NLS-BirA-2A-mCherry_Ras

   Plasmid

   #80059

   Purpose

   MCS for cloning promoter to drive HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Use

   Zebrafish biotagging

   Tags

   3x HA

   Expression

   Bacterial

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Cas9_YTHDF2_sgRNA

    Plasmid

    #186673

    Purpose

    YTHDF2 sgRNA plasmid

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    YTHDF2 KO sgRNA Plasmid (YTHDF2 Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV-GABARα1N-HA

    Plasmid

    #128110

    Purpose

    Express GABARα1 N terminal domain

    Depositor

    Huda Zoghbi

    Article

    Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

    Insert

    Gabra1 (Gabra1 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGEM-BirA-2A-H2B-Dendra2-SV40pA-FKF

    Plasmid

    #119864

    Purpose

    Donor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), H2B-tagged Dendra2 fluorophore and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

    Depositor

    Tatjana Sauka-Spengler

    Article

    Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d

    Insert

    BirA, 2A, H2B-tagged Dendra2 and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    

    Tags

    HA-tagged biotin ligase (BirA), Thosea asigna vir…

    Expression

    Bacterial

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Mouse GFP-PRMT7 LDIG(69-72)AAAA

    Plasmid

    #65044

    Purpose

    Expresses Catalytically inactive GFP-PRMT7 LDIG(69-72)AAAA mutant

    Depositor

    Steven Clarke

    Article

    Feng et al J Biol Chem. 2013 Dec 27;288(52):37010-2

    Insert

    PRMT7 (Prmt7 Mouse)
    

    Use

    Bacterial propagation

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    L69A D70A I71A G72A

    Promoter

    CMV

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

14. tdEos-Keratin-17

    Plasmid

    #57629

    Purpose

    Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

    Insert

    Keratin (KRT18 Human)
    

    Tags

    tdEos

    Expression

    Mammalian

    Mutation

    V282L

    Promoter

    CMV

    Available Since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

15. pHIVT9-NAT-3xFlag-SMARCAL1-ΔN

    Plasmid

    #246936

    Purpose

    Lentiviral vector that expresses Flag-tagged SMARCAL1 with a deletion of the RPA-binding domain located in the N-terminal region (ΔN)

    Depositor

    Daniel Durocher

    Article

    Feng et al Mol Cell. 2024 Dec 5;84(23):4522-4537.e7

    Insert

    SMARCAL1 (SMARCAL1 Human)
    

    Use

    Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Promoter

    EF-1-alpha promoter

    Available Since

    Dec. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHIVT9-NAT-3xFlag-SMARCAL1-R764Q

    Plasmid

    #246937

    Purpose

    Lentiviral vector that expresses Flag-tagged SMARCAL1 with a mutation in the ATP translocase domain (R764Q)

    Depositor

    Daniel Durocher

    Article

    Feng et al Mol Cell. 2024 Dec 5;84(23):4522-4537.e7

    Insert

    SMARCAL1 (SMARCAL1 Human)
    

    Use

    Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Promoter

    EF-1-alpha promoter

    Available Since

    Dec. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHIVT9-NAT-3xFlag-SMARCAL1

    Plasmid

    #246935

    Purpose

    Lentiviral vector that expresses wildtype Flag-tagged SMARCAL1

    Depositor

    Daniel Durocher

    Article

    Feng et al Mol Cell. 2024 Dec 5;84(23):4522-4537.e7

    Insert

    SMARCAL1 (SMARCAL1 Human)
    

    Use

    Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Promoter

    EF-1-alpha promoter

    Available Since

    Dec. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHNF4A-Enh_Gsta2-IRES2-H2BeGFP

    Plasmid

    #247049

    Purpose

    This plasmid was employed to generate the mouse model used in this study. It contains the human HNF4A gene enhancer linked to the Shh mini-promoter and the Gsta2-IRES2-H2BeGFP. Homologous arm

    Depositor

    Andrew McMahon

    Article

    Cheng et al J Am Soc Nephrol. 2025 Aug 21. doi: 10.1

    Insert

    Glutathione S-Transferase Alpha 2 (Gsta2 Mouse)
    

    Expression

    Mammalian

    Available Since

    Nov. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MSCV-IRES-Thy1.1-Aoah

    Plasmid

    #233141

    Purpose

    Thy1.1 and Aoah overexpression

    Depositor

    Peng Jiang

    Article

    Gong et al Cell. 2025 Jul 24:S0092-8674(25)00793-7.

    Insert

    Acyloxyacyl hydrolase (Aoah Mouse)
    

    Use

    Lentiviral

    Available Since

    Nov. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Egr1-1POK(E239Y,L349K)-dMBP-V5

    Plasmid

    #239428

    Purpose

    Egr1 signal monomer

    Depositor

    Changyang Linghu

    Article

    Zheng et al bioRxiv 2025.05.10.653182

    Insert

    CytoTape-V5 (EGR1 Synthetic)
    

    Use

    AAV

    Tags

    V5-dMBP

    Expression

    Mammalian

    Promoter

    Egr1 promoter

    Available Since

    Oct. 29, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge