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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,837 results for: pET

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Showing: 6061 - 6080 of 9837 results

  3. pACB120

    Plasmid
    #222213
    Purpose
    For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with five mutations (K10N, S11R, W15C, G76V, G258D) and a C-terminal His tag
    Depositor
    Gregg Beckham
    Article
    Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.
    Insert
    fghA(K10N, S11R, W15C, G76V, G258D)
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Contains five amino acid mutations: K10N, S11R, W…
    Promoter
    T7
    Available Since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    222213-plasmid-map-sequence-id-443111

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  4. pACB143

    Plasmid
    #222214
    Purpose
    For recombinant expression of vanB (PP_3737 from Pseudomonas putida) with the A24P mutation and an N-terminal thrombin-cleavable His tag
    Depositor
    Gregg Beckham
    Article
    Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.
    Insert
    vanB(A24P)
    Tags
    N-terminal, thrombin-cleavable 6x His tag
    Expression
    Bacterial
    Mutation
    Changed Alanine 24 to Proline
    Promoter
    T7
    Available Since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    222214-plasmid-map-sequence-id-438176

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  6. pACB109

    Plasmid
    #222208
    Purpose
    For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the K10N mutation and a C-terminal His tag
    Depositor
    Gregg Beckham
    Article
    Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.
    Insert
    fghA (K10N)
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Changed Lys10 to Asn
    Promoter
    T7
    Available Since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    222208-plasmid-map-sequence-id-437369

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  7. pACB110

    Plasmid
    #222209
    Purpose
    For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the G258D mutation and a C-terminal His tag
    Depositor
    Gregg Beckham
    Article
    Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.
    Insert
    fghA (G258D)
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Changed Gly258 to Aspartate
    Promoter
    T7
    Available Since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    222209-plasmid-map-sequence-id-437372

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  8. pACB111

    Plasmid
    #222210
    Purpose
    For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the G76V mutation and a C-terminal His tag
    Depositor
    Gregg Beckham
    Article
    Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.
    Insert
    fghA(G76V)
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Changed glycine 76 to valine
    Promoter
    T7
    Available Since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    222210-plasmid-map-sequence-id-437376

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  9. pACB112

    Plasmid
    #222211
    Purpose
    For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the S11R mutation and a C-terminal His tag
    Depositor
    Gregg Beckham
    Article
    Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.
    Insert
    fghA(S11R)
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Changed Serine 11 to Arginine
    Promoter
    T7
    Available Since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    222211-plasmid-map-sequence-id-437378

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  10. pACB113

    Plasmid
    #222212
    Purpose
    For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the W15C mutation and a C-terminal His tag
    Depositor
    Gregg Beckham
    Article
    Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.
    Insert
    fghA(W15C)
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Changed Tryptophan 15 to Cysteine
    Promoter
    T7
    Available Since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    222212-plasmid-map-sequence-id-437381

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  19. Pfu-MBP

    Plasmid
    #215156
    Purpose
    Encodes a thermostable maltose binding protein
    Depositor
    Travis Beddoe, Bhuvana Shanbhag
    Insert
    Pfu-MBP
    Expression
    Bacterial
    Promoter
    T7 lac
    Available Since
    June 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    215156-plasmid-map-sequence-id-430149

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  20. MBP-YCup1

    Plasmid
    #215157
    Purpose
    Encodes a thermostable maltose binding protein fused to yeast copper metallothionein
    Depositor
    Travis Beddoe, Bhuvana Shanbhag
    Insert
    MBP-YCup1
    Expression
    Bacterial
    Promoter
    T7 lac
    Available Since
    June 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    215157-plasmid-map-sequence-id-430150

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Showing: 6061 - 6080 of 9837 results