We narrowed to 8,876 results for: sgRNA

Showing: 6121 - 6140 of 8876 results

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pSWAP_Entry_T1

Plasmid

#184863

Purpose

pSwap plasmid part containing the T1 sgRNA module for the Swap and Drop recombination system.

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available Since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pgRNA

Plasmid

#215560

Purpose

Constitutively expressed sgRNA targeting a desired gene (this case pta), ColE1 origin, KanR

Depositor

Brian Pfleger

Article

Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

Insert

gRNA

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA Puro

Plasmid

#167911

Purpose

Lentiviral vector for expressing U6 sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCFD5-NS

Plasmid

#149546

Purpose

Expression of pegRNA(s) and sgRNA(s) under control of Drosophila U6:3 promoter. NS stands for "No Scaffold". Can be used to generate transgenic flies with vermillion+ selection.

Depositor

Norbert Perrimon

Article

Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Type

Empty backbone

Use

CRISPR

Expression

Insect

Promoter

U6

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pICSL1P1_AtU626Ter26

Plasmid

#191769

Purpose

Cas9 guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

SpCas9 guide scaffold with AtU626 promoter/terminator

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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px459 VQR

Plasmid

#101715

Purpose

sgRNA/Cas9 expression plasmid with Cas9 VQR mutations (NGA PAM)

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

SpCas9 VQR

Tags

3xFLAG-NLS and NLS

Expression

Mammalian

Mutation

VQR (D1135V, R1335Q and T1337R)

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pAbaMCi-BsaI_pJMP2776

Plasmid

#208890

Purpose

Acinetobacter baumannii CRISPRi plasmid for cloning new guides (BsaI cloning site in gRNA cassette).

Depositor

Jason Peters

Article

Ward et al mBio. 2023 Dec 21:e0205123. doi: 10.1128

Insert

dCas9, sgRNA expression cassette

Expression

Bacterial

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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LRT2B-GO2

Plasmid

#136909

Purpose

Lentivirus expressing GO2 sgRNA

Depositor

Lukas Dow

Article

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

sgGO2

Use

Lentiviral

Mutation

WT

Promoter

hU6

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCR8-attR4-pegRNA-attR3

Plasmid

#213056

Purpose

Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attR4 and attR3

Depositor

Bing Yang

Article

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Insert

sgRNA

Expression

Bacterial

Promoter

35S-CmYCLV-AtU6

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCR8-attL5-pegRNA-attL4

Plasmid

#213055

Purpose

Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL5 and attL4

Depositor

Bing Yang

Article

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Insert

sgRNA

Expression

Bacterial

Promoter

35S-CmYCLV-AtU6

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCR8-attL1-pegRNA-attR5

Plasmid

#213053

Purpose

Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attR5

Depositor

Bing Yang

Article

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Insert

sgRNA

Expression

Bacterial

Promoter

35S-CmYCLV-AtU6

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pMM704

Plasmid

#68891

Purpose

IPTG-inducible CRISPRi vector targeting BT1854, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Inserts

dCas9
LacI
sgRNA

Available Since

Oct. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA mcherry

Plasmid

#167910

Purpose

Lentiviral vector for expressing U6 sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA EYFP

Plasmid

#167906

Purpose

Lentiviral vector for expressing U6 sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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px458_Conc2

Plasmid

#134449

Purpose

Empty concatemer vector in which 2 sgRNAs can be inserted; with Cas9 + GFP

Depositor

Daniel Stange

Article

Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-Stuffer-HepmCherry

Plasmid

#192825

Purpose

Contains stuffer sequence into which sgRNAs can be cloned and expresses mCherry from hepatocyte-specific promoter

Depositor

Kristin Knouse

Article

Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

Insert

mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Hepatocyte-specific promoter (HS-CRM8-TTRmin modu…

Available Since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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p8267 LentiCRISPR v2 hygro sgNT-2

Plasmid

#193978

Purpose

Expression of spCas9 and non-targeting control sgRNA

Depositor

Elizabeth White

Article

Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

Insert

spCas9

Use

Lentiviral

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pAFi-dHdrA

Plasmid

#184907

Purpose

carrying CRISPR/dCas9 system for gene knockdown in Acidithiobacillus ferridurans

Depositor

Radhakrishnan Mahadevan

Article

Chen et al Environ Sci Technol. 2023 Aug 22;57(33):

Insert

Cm-sgRNA-dCas9

Use

CRISPR

Expression

Bacterial

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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PX458_RAD21_1

Plasmid

#64057

Purpose

Expresses gRNA against human RAD21 along with Cas9 with 2A GFP

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA

Use

CRISPR

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pMM733

Plasmid

#68898

Purpose

IPTG-inducible CRISPRi vector targeting NanoLuc (NL4), constiutive NanoLuc, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Inserts

dCas9
LacI
sgRNA
NanoLuc

Available Since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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