We narrowed to 8,517 results for: sgRNA

1. pAAV-FLEX-SaCas9-U6-sgKcnab2

   Plasmid

   #192795

   Purpose

   Encodes SaCas9 and an sgRNA targeting mouse Kcnab2

   Depositor

   Marta Soden

   Article

   Yee et al J Neurophysiol. 2022 Jul 1;128(1):62-72.

   Insert

   sgKcnab2
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCF713

   Plasmid

   #121665

   Purpose

   U6-sgRNA EF1a-ProCas9-FlaviS6-P2A-Puro

   Depositor

   David Savage

   Article

   Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

   Inserts

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. AP568-2

   Plasmid

   #70047

   Purpose

   co-expression of Cas9 and crRNA 589 target sequence

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

   Insert

   sgRNA for dpy‐10
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Oct. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. px330 EQR

   Plasmid

   #101733

   Purpose

   Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 EQR
   

   Use

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   EQR (D1135E, R1335Q, and T1337R mutations in Cas9)

   Promoter

   Available since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330.iGFP1

   Plasmid

   #66581

   Purpose

   sgRNA for iGFP

   Depositor

   Wen Xue

   Article

   Li et al Genome Biol. 2015 May 28;16(1):111.

   Insert

   iGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 27, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pX330.iGFP2

   Plasmid

   #66582

   Purpose

   sgRNA for iGFP

   Depositor

   Wen Xue

   Article

   Li et al Genome Biol. 2015 May 28;16(1):111.

   Insert

   iGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 27, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pX330.iGFP5

   Plasmid

   #66584

   Purpose

   sgRNA for iGFP

   Depositor

   Wen Xue

   Article

   Li et al Genome Biol. 2015 May 28;16(1):111.

   Insert

   iGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pX330.LoxPO

   Plasmid

   #66585

   Purpose

   sgRNA for LSL

   Depositor

   Wen Xue

   Article

   Li et al Genome Biol. 2015 May 28;16(1):111.

   Insert

   LSL-Tomato
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 27, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pEPOR2KN0079

   Plasmid

   #117646

   Purpose

   Level2 MoClo construct, containing level1 plant expression cassettes for SaCas9(pEPOR1CB0015) and sgRNA_ENTH/ANT {AT1G14910}(pEPOR1CB0100)and sgRNA_ENTH/ANT {AT2G01600}(pEPOR1CB0111)

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   [35S:SaCas9(pEPOR1CB0015) ] +[AtU6-26:sgRNA]
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330.iGFP3

    Plasmid

    #66583

    Purpose

    sgRNA for iGFP

    Depositor

    Wen Xue

    Article

    Li et al Genome Biol. 2015 May 28;16(1):111.

    Insert

    iGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pSJ107-2x

    Plasmid

    #208812

    Purpose

    Expresses SoxS activation domainand gRNA targeting two copies of 107 region within the synthetic promoter in bacterial cell

    Depositor

    Marc Erhardt

    Article

    Naseri et al Commun Biol. 2023 Oct 3;6(1):1005. doi:

    Insert

    sgRNA 107-2x_mRFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    CRISPR/dCas9-responsive synthetic promoter

    Available since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSJ106-2x

    Plasmid

    #208860

    Purpose

    Expresses SoxS activation domain and gRNA targeting two copies of 106 region within the synthetic promoter in bacterial cell

    Depositor

    Marc Erhardt

    Article

    Naseri et al Commun Biol. 2023 Oct 3;6(1):1005. doi:

    Insert

    sgRNA 106-2x_mRFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    CRISPR/dCas9-responsive synthetic promoter

    Available since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAS458-U6_CBA-mCherry

    Plasmid

    #195566

    Purpose

    Transient expression of mCherry; U6 with scaffold for sgRNA cloning in mammalian cells.

    Depositor

    Joanna Wysocka

    Article

    Spencley et al Mol Cell. 2023 May 18;83(10):1623-1639.e

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCambia3301-AtU6-MAR1

    Plasmid

    #210757

    Purpose

    Mutagenesis of the Arabidopsis MAR1 gene for efficient screening.

    Depositor

    Daisuke Miki

    Article

    Cheng et al Sci Rep. 2024 Dec 28;14(1):30791. doi: 1

    Insert

    AtU6-26 pro::MAR1 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtU6-26

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Afp

    Plasmid

    #99696

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Afp, vector allows for strong activation of mouse Afp, can be packaged and delivered as AAV

    Depositor

    George Church

    Insert

    dCas9 and gRNA targeting Afp (Afp Synthetic)
    

    Use

    AAV

    Tags

    VPR mini

    Expression

    Mutation

    dead Cas9

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSCL452

    Plasmid

    #219502

    Purpose

    Golden Gate entry vector for library cloning of msd-sgRNA arrays: GAL7p-Csy4-PaqCI-PaqCI-GAL7t // SNR52p-msr-SUP4t

    Depositor

    Seth Shipman

    Article

    Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

    Insert

    GAL7p-Csy4-PaqCI-PaqCI-GAL7t // SNR52p-msr-SUP4t
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL7

    Available since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSK-Spsg

    Plasmid

    #214351

    Purpose

    Expresses cloning backbone for SpCas9 sgRNA

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SpCas9 tracrRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUC-ODN-hsRPL3-CterTwStrep-ΔPAM62&56

    Plasmid

    #208645

    Purpose

    Remove PAM region in HomoArms by introduce SNP rs7495557 and rs760194863, to prevent cleavage after success edit. Digest with nt.BspQI and nb.BsrDI to get ssDNA.

    Depositor

    Quanfu Ma

    Insert

    Twin Strep tag with RPL3 genome HomoArm (RPL3 Synthetic, Human)
    

    Use

    CRISPR; Ssdna donnor preparation for knock-in of…

    Tags

    Twin Strep

    Expression

    Mutation

    Two SNP (rs749555789, rs760194863) were introduce…

    Promoter

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDGO186N-KS1

    Plasmid

    #174301

    Purpose

    Replicating plasmid with sgRNA cassette containing a killing spacer #1 targeting the wild type polC gene.

    Depositor

    Lee Lynd

    Article

    Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

    Insert

    spacer expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDGO186N-KS2

    Plasmid

    #174302

    Purpose

    Replicating plasmid with sgRNA cassette containing a killing spacer #2 targeting the wild type polC gene.

    Depositor

    Lee Lynd

    Article

    Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

    Insert

    spacer expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge