We narrowed to 8,829 results for: sgRNA

1. pBLO1808_arC9_2xNLS_human

   Plasmid

   #74491

   Purpose

   Human codon optimized plasmid to express arC9 t2a mCherry w/2xNLS and a sgRNA

   Depositor

   David Savage

   Article

   Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

   Insert

   arC9
   

   Tags

   t2a mCherry

   Expression

   Mammalian

   Available Since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. px459V2.0_Conc3

   Plasmid

   #134458

   Purpose

   Empty concatemer vector in which 3 sgRNAs can be inserted; with Cas9 + Puro

   Depositor

   Daniel Stange

   Article

   Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSB700 lenti gRNA Puro_zhang2.0

   Plasmid

   #167919

   Purpose

   Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAbaMCi-GFP-gfp_pJMP2768

   Plasmid

   #208889

   Purpose

   Acinetobacter baumannii CRISPRi test plasmid (GFP, gfp-targeting gRNA).

   Depositor

   Jason Peters

   Article

   Ward et al mBio. 2023 Dec 21:e0205123. doi: 10.1128

   Insert

   dCas9, sgRNA expression cassette, sfGFP
   

   Expression

   Bacterial

   Available Since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBLO1806_Cas9_2xNLS_human

   Plasmid

   #74493

   Purpose

   Human codon optimized plasmid to express Cas9 t2a mCherry w/2xNLS and a sgRNA

   Depositor

   David Savage

   Article

   Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

   Insert

   Cas9
   

   Tags

   t2a mCherry

   Expression

   Mammalian

   Available Since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSB700 lenti gRNA Hygro

   Plasmid

   #167907

   Purpose

   Lentiviral vector for expressing U6 sgRNA cloning plasmid

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMM710

   Plasmid

   #68893

   Purpose

   IPTG-inducible CRISPRi vector targeting nonsense sequence (NS), pNBU2 backbone, AmpR

   Depositor

   Timothy Lu, Christopher Voigt

   Article

   Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

   Inserts

   dCas9
   LacI
   sgRNA
   

   Available Since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSB700 lenti gRNA iRFP713

   Plasmid

   #167908

   Purpose

   Lentiviral vector for expressing U6 sgRNA cloning plasmid

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDCC5

   Plasmid

   #59984

   Purpose

   Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9-D10A nickase and U6>sgRNA cassette

   Depositor

   Peter Duchek

   Article

   Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

   Insert

   Cas9-D10A
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Insect

   Mutation

   D10A

   Promoter

   hsp70Bb

   Available Since

   Oct. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. PX458_CEBPB_1

    Plasmid

    #64036

    Purpose

    Expresses gRNA against human CEBPB along with Cas9 with 2A GFP

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    July 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. PX458_CEBPB_2

    Plasmid

    #64047

    Purpose

    Expresses gRNA against human CEBPB along with Cas9 with 2A GFP

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. GEARBOCS-SPARCL1-N-HATag

    Plasmid

    #218185

    Purpose

    To tag Hevin with HA at the N-terminal

    Depositor

    Cagla Eroglu

    Article

    Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

    Insert

    sgRNA (Sparcl1 Mouse)
    

    Use

    AAV and CRISPR

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. GEARBOCS-SPARCL1-C-mCherryTag

    Plasmid

    #218183

    Purpose

    To tag Hevin with mCherry at its C-terminal

    Depositor

    Cagla Eroglu

    Article

    Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

    Insert

    sgRNA (Sparcl1 Mouse)
    

    Use

    AAV and CRISPR

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSB700 lenti gRNA iRFP713_zhang2.0

    Plasmid

    #167916

    Purpose

    Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHX_gRNA_hPAX7_6-9S

    Plasmid

    #160453

    Purpose

    sgRNA targeting human PAX7 3' UTR with 17bp spacer

    Depositor

    April Pyle

    Article

    Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

    Insert

    gRNA_hPAX7_6-9S
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. HA-GFP_rescue_construct

    Plasmid

    #190641

    Purpose

    Puromycin-selectable expression of GFP in Drosophila S2 cells

    Depositor

    David Bartel

    Article

    Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

    Insert

    EGFP
    

    Tags

    3xHA

    Expression

    Insect

    Promoter

    Actin-5c

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. LRT2B-AdGO

    Plasmid

    #136910

    Purpose

    Lentivirus expressing AdGO sgRNA

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    sgAdGO
    

    Use

    Lentiviral

    Mutation

    WT

    Promoter

    hU6

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. lentiCRISPRv2-Blast-mU6

    Plasmid

    #206806

    Purpose

    pLentiCRISPRv2 variant with mU6 promoter driving the sgRNA, and a Blasticidin resistance cassette

    Depositor

    Jolanda Van Leeuwen

    Article

    Unlu et al Genome Med. 2023 Oct 12;15(1):78. doi: 1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSB700 lenti gRNA EBFP2

    Plasmid

    #167905

    Purpose

    Lentiviral vector for expressing U6 sgRNA cloning plasmid

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMH183

    Plasmid

    #122856

    Purpose

    MS2-modified guide RNA targeting the FT promoter with GreenGate D-E flanking sequences

    Depositor

    Markus Schmid

    Article

    Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

    Insert

    MS2-modified sgRNA targeting the Arabidopsis FT promoter
    

    Use

    Golden gate compatible cloning vector

    Available Since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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