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pBABE-puro SV40 LT

Plasmid

#13970

Depositor

Thomas Roberts

Article

Zhao et al Cancer Cell. 2003 May . 3(5):483-95.

Insert

SV40 LT (SV40gp6 )

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only

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T7-VEE-IRES-Puro

Plasmid

#58970

Purpose

noninfectious (non-packaging), self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

Depositor

Steven Dowdy

Article

Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

Type

Empty backbone

Use

Venezuelan equine encephalitis (vee) virus rna re…

Expression

Mammalian

Promoter

26S subgenomic promoter

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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attP-puro donor

Plasmid

#182139

Purpose

attP-puro DNA donor for twinPE and Bxb1 mediated gene-size insertion

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

attP-EGFP-EF1α-PuroR-T2A-TagBFP

Expression

Mammalian

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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lentiGuideFE-mSG-Puro

Plasmid

#226522

Purpose

Cas9 guide RNA scaffold with the F+E scaffold modification with mStayGold-P2A-Puro

Depositor

Neville Sanjana

Article

Caragine et al Nat Commun. 2025 Feb 13;16(1):1611. doi:

Insert

hU6_SpCas9gRNAF+E_EF1a_mStayGold_P2A_Puro

Use

CRISPR and Lentiviral

Tags

mStayGold

Expression

Mammalian

Promoter

U6,EF1a

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-shRPS6

Plasmid

#125780

Purpose

constitutive expression of a short-hairpin RNA targeting human RPS6 (RNAi positive control)

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

shRPS6 (RPS6 Human)

Use

RNAi

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti puro HA-Ubiquitin

Plasmid

#74218

Purpose

Lentiviral expression of HA tagged Ubiquitin

Depositor

Melina Fan

Insert

ubiquitin (UBB Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Single ubiquitin coding sequence (full length UBB…

Promoter

CMV

Available Since

March 14, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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Lenti NG-ABE8e_P2A_GFP + PuroR

Plasmid

#242000

Purpose

Lentiviral plasmid for generation of cell lines stably expressing NG-ABE8e base editor. NG-ABE8e expression can be followed by GFP fluorescence. Contains PGK-puromycin resistance cassette.

Depositor

Frank Buchholz

Article

Wang et al Genome Biol. 2025 Jul 22;26(1):217. doi:

Insert

NG-ABE8e

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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Lenti-EF1alpha-ZsGreen1_Puro

Plasmid

#192661

Purpose

3rd generation lenti vector encoding ZsGreen1 with 2A Puro resistance marker

Depositor

Jeffrey Miner

Insert

ZsGreen1

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pUBC-Pfv-Sapphire_PURO

Plasmid

#203651

Purpose

Mammalian lentiviral vector for the expression of the Sapphire-tagged 40nm GEM under the UBC promoter, with a puromycin selection marker.

Depositor

Liam Holt

Article

Shu T et et al bioRxiv 2022

Insert

PfV

Use

Lentiviral

Tags

Sapphire

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-eGFP-mcGAS

Plasmid

#108675

Purpose

For production of retrovirus and transduction of mammalian cells, allowing expression of N-terminally eGFP-tagged mouse cGAS

Depositor

Andrew Jackson, Martin Reijns

Article

Mackenzie et al Nature. 2017 Aug 24;548(7668):461-465. d

Insert

eGFP-cGAS (Cgas Mouse)

Use

Retroviral

Promoter

LTR

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pBABE-puro-hTERT

Plasmid

#1771

Purpose

Retroviral expression of hTERT. Used to create immortalized cell lines.

Depositor

Bob Weinberg

Article

Counter et al Proc Natl Acad Sci U S A 1998 Dec 8;95(2

Insert

hTERT (TERT Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 3, 2005

Availability

Academic Institutions and Nonprofits only

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pirespuro2-CTLA4-mEos2

Plasmid

#98285

Purpose

mammalian expression of CTLA4-mEos2

Depositor

Mike Heilemann

Article

Fricke et al Sci Rep. 2015 Sep 11;5:14072. doi: 10.10

Insert

CTLA4 (CTLA4 Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pBABE-puro HGF

Plasmid

#10901

Depositor

Bob Weinberg

Article

Gupta et al Nat Genet. 2005 Oct . 37(10):1047-54.

Insert

HGF (HGF Human)

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

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pLVpuro-CMV-N-mCherry

Plasmid

#123221

Purpose

N-terminal mCherry tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Type

Empty backbone

Use

Lentiviral; Gateway destination

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 18, 2019

Availability

Academic Institutions and Nonprofits only

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CROPseq-Guide-Puro

Plasmid

#86708

Purpose

Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the puromycin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.

Depositor

Christoph Bock

Article

Datlinger et al Nat Methods. 2017 Jan 18. doi: 10.1038/n

Insert

EF1a-Puro-WPRE-hU6-gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pIRES-Puro-MGMT

Plasmid

#187654

Purpose

Expresses hMGMT together with the puromycin resistance gene via an IRES.

Depositor

Robert Sobol

Article

Li et al Cancers (Basel). 2022 Jul 22;14(15). pii

Insert

MGMT (MGMT Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 puro Nodamura B2

Plasmid

#17228

Depositor

Christopher Sullivan

Article

Sullivan et al J Virol. 2005 Jun . 79(12):7371-9.

Insert

Nodamura B2 (NoVsRNA1gp2 Nodamura Virus)

Expression

Mammalian

Mutation

2 silent point mutations

Available Since

June 22, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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T-2A-EGFP-PGK-Puro

Plasmid

#83344

Purpose

Donor vector to knocin EGFP into human Brachyury(T) locus

Depositor

James Thomson

Article

Chu et al Genome Biol. 2016 Aug 17;17(1):173. doi:

Insert

Human Brachyury knockin homology arms (TBXT Human)

Tags

2A-EGFP

Available Since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pINDUCER21-puro_Gateway-3xFLAG

Plasmid

#172981

Purpose

Gateway Destination vector for doxycycline-inducible expression of ORF

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Type

Empty backbone

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pLX302 OAS1-V5 puro

Plasmid

#158641

Purpose

Lentiviral expression vector for constitutive expression of V5-tagged OAS1.

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

OAS1 (OAS1 Human)

Use

Lentiviral

Tags

V5

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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