We narrowed to 16,185 results for: grna

1. pW193-lenti-sasgRNA-lacZ-pEF1s-NLS-mNeonGreen-P2A-BlastR

   Plasmid

   #170814

   Purpose

   Lentiviral vector to co-express a lacZ control sasgRNA with NLS-mNeonGreen

   Depositor

   Kenneth Yamada

   Article

   Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

   Insert

   NLS-mNeonGreen-P2A-BlastR
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   NA

   Available Since

   June 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBBK01 pCAS-Tyr-[gRNA: BsaI GFP dropout] (KanR, AmpR)

   Plasmid

   #178985

   Purpose

   Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Kanamycin resistance

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Available Since

   May 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBBK05 pCAS-Tyr-[gRNA: BsaI GFP dropout] (NatR,AmpR)

   Plasmid

   #178989

   Purpose

   Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Nourseothricin resistance

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Available Since

   May 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Lenti-L227R-tevopreQ1-epegRNA+13C>A_EF1a-puroR (PBS 10 - RTT 19)

   Plasmid

   #207357

   Purpose

   Lentiviral transfer plasmid encoding hU6-driven expression of a L227R-CFTR correcting prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.

   Depositor

   Marianne Carlon

   Article

   Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

   Insert

   L227R-CFTR + 13 C>A tevopreq1-prime editing guide RNA (epegRNA)
   

   Use

   Lentiviral

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJEP15-AAV-H1/TO-sgRNA(Tet2)-CMV-TetR-P2A-eGFP-KASH-pA

   Plasmid

   #82701

   Purpose

   AAV backbone with a minimal H1/TO promoter driving expression of a sgRNA that targets exon 3 of the mouse Tet2 locus. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.

   Depositor

   Jonathan Ploski

   Article

   de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do

   Insert

   H1/TO gRNA Expression Cassette Containing Tet2 gRNA
   

   Use

   AAV and CRISPR

   Tags

   CMV promoter driving expression of TetR-P2A-GFP-K…

   Expression

   Mammalian

   Available Since

   Dec. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. JI501: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::SaCas9 gRNA scaffold

   Plasmid

   #121841

   Purpose

   pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; empty hU6-driven SaCas9 gRNA scaffold for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to dCas9 w/ gRNA expression

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMB1052:miniTol2-U6-(empty)SaCas9_gRNA-CAG-Cre-P2A-NeoR-WPRE

   Plasmid

   #168108

   Purpose

   Tol2 transposon vector of empty U6-S.aureus Cas9 gRNA cassette and CAG-driven Cre-NeoR

   Depositor

   Erich Jarvis

   Article

   Biegler et al Sci Rep. 2022 Mar 14;12(1):4369. doi: 10

   Type

   Empty backbone

   Use

   CRISPR and Cre/Lox

   Available Since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pW301-lenti-spsgRNA-hsITGB1-pEF1s-NLS-mNeonGreen-P2A-BlastR

   Plasmid

   #170817

   Purpose

   Lentiviral vector to co-express a human ITGB1 spsgRNA with NLS-mScarlet-I

   Depositor

   Kenneth Yamada

   Article

   Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

   Insert

   NLS-mNeonGreen-P2A-BlastR
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   NA

   Available Since

   June 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-eCas9-T2A-EGFP-U6-gRNA targeting Nrl-no ITR and f1 ori

   Plasmid

   #234737

   Purpose

   All-in-one CRISPR/Cas9 vector encodes high-fidelity eSpCas9 and a gRNA targeting Nrl, efficiently reprogramming rod precursors into cone-like cells in the mouse retina.

   Depositor

   Kirill Martemyanov

   Article

   Zhao et al Nat Commun. 2025 Apr 22;16(1):3768. doi:

   Insert

   Nrl (Nrl Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV hU6-VEGFR2#3 sgRNA Nestin-dCas9-KRAB-T2a-GFP

    Plasmid

    #196990

    Purpose

    Derived from pLV hU6-sgRNA Nestin-dCas9-KRAB-T2a-GFP with KDR sgRNA

    Depositor

    Elizabeth Kirby

    Article

    Dause et al Mol Neurobiol. 2025 Mar 25. doi: 10.1007

    Insert

    VEGFR2
    

    Use

    CRISPR and Lentiviral

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-MYL2-TadA8e-SpN aa2-713- inteinN-U6-Camk2d sgRNA7

    Plasmid

    #226915

    Purpose

    Expresses TadA8e and Sp cas9N by MYL2 promoter and sgRNA targeting murine Camk2d intron7 5' splice site by U6 promoter

    Depositor

    Yuxuan Guo

    Article

    Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

    Insert

    MYL2, TadA, nSp Cas9N, inteinN
    

    Use

    AAV

    Promoter

    MYL2

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-MYL2-HA-TadA8e-Sauri N aa1-438-inteinN-U6-Camk2d sgRNA7

    Plasmid

    #226678

    Purpose

    Expresses TadA8e and Sauri cas9N by MYL2 promoter and sgRNA targeting murine Camk2d intron7 5' splice site by U6 promoter

    Depositor

    Yuxuan Guo

    Article

    Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

    Insert

    MYL2, TadA, nSauri Cas9N, inteinN
    

    Use

    AAV

    Promoter

    MYL2

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV-RnSyt1-sgRNA2-dCas9-KRAB-TagBFP2 (identifier AAAA-0246)

    Plasmid

    #202555

    Purpose

    Synaptotagmin-1 sgRNA2 with TagBFP2

    Depositor

    Frederic Meunier

    Article

    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

    Insert

    CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ - CACCAGTACTCGCGTGCCTCGCACCGG) (Syt1 Rat)
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV-RnSyt1-sgRNA3-dCas9-KRAB-TagBFP2 (Identifier AAAA-0247)

    Plasmid

    #202556

    Purpose

    Synaptotagmin-1 sgRNA3 with TagBFP2

    Depositor

    Frederic Meunier

    Article

    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

    Insert

    CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ - CACCTCCTCCTGCAGCGGCAGCATCGG) (Syt1 Rat)
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLV-RnSyt1-sgRNA1-dCas9-KRAB-TagBFP2 (identifier AAAA-0245)

    Plasmid

    #202554

    Purpose

    Synaptotagmin-1 sgRNA1 with TagBFP2

    Depositor

    Frederic Meunier

    Article

    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

    Insert

    CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ -CACCGCGTGCCTCGCACCGGTCCGCGG) (Syt1 R. norvegius (gRNA))
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBBK02 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitKanR, AmpR)

    Plasmid

    #178986

    Purpose

    Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitKanamycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBBK10 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitURA3,AmpR)

    Plasmid

    #178994

    Purpose

    Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitURA3

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBBK08 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitLEU2,AmpR)

    Plasmid

    #178992

    Purpose

    Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitLEU2

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBBK06 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitNatR,AmpR)

    Plasmid

    #178990

    Purpose

    Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitNourseothrycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pW188-lenti-spsgRNA-lacZ-pEF1s-NLS-mScarlet-I-P2A-BlastR

    Plasmid

    #170813

    Purpose

    Lentiviral vector to co-express a lacZ control spsgRNA with NLS-mScarlet-I

    Depositor

    Kenneth Yamada

    Article

    Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

    Insert

    NLS-mScarlet-I-P2A-BlastR
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    NA

    Available Since

    June 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge