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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,593 results for: nls

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Showing: 6201 - 6220 of 6593 results

  1. U6_ ATM_G101_sgRNA_CAG_St1Cas9_CNRZ1066_v2

    Plasmid
    #214814
    Purpose
    A single vector containing a CAG-driven Cas9 variant from S. thermophilus recognizing a consensus NNACAA PAM (St1Cas9 CNRZ1066 v2) and its U6-driven sgRNA targeting human ATM
    Depositor
    Yannick Doyon
    Insert
    St1Cas9 CNRZ1066 v2 targeting ATM (ATM Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Feb. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    214814-plasmid-map-sequence-id-422874

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  5. AA195

    Plasmid
    #215984
    Purpose
    Fragmid fragment: (N' terminus) ALFA tag for recruiting via NbALFA
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    Start; BPNLS_v1.1; ALFA_v1.3; ALFA_v1.2; ALFA_v1.1; ALFA_v1.4; ALFA_v1.5
    Use
    Fragment
    Available Since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215984-plasmid-map-sequence-id-431454

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  7. AA125

    Plasmid
    #215982
    Purpose
    Fragmid fragment: (N' terminus) activator from p65 subunit of mouse Nfkb
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    Start; p65_v1.3; HSF1_v1.2; Linker_v3.1; SV40NLS_v1.6
    Use
    Fragment
    Available Since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215982-plasmid-map-sequence-id-431448

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  9. Coexpressed mRuby3 VLP

    Plasmid
    #182431
    Purpose
    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-GFP (GAL10 promoter), and VP2C-mRuby3 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al bioRxiv 2022.11.24.517869
    Inserts
    VP2C-GFP
    deltaVP1
    VP2C-mRuby3
    Use
    Synthetic Biology
    Expression
    Yeast
    Promoter
    GAL1, GAL10, and GAL7
    Available Since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182431-plasmid-map-sequence-id-360113

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  10. pSCL.178

    Plasmid
    #184999
    Purpose
    Express -Eco1 FANCF editing ncRNA and gRNA
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1: FANCF targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    Tags
    SV40NLS
    Expression
    Mammalian
    Mutation
    FANCF donor, a1/a2 length extended for 27 bp
    Promoter
    H1
    Available Since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    184999-plasmid-map-sequence-id-376704

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  11. pSCL.176

    Plasmid
    #184997
    Purpose
    Express -Eco1 RNF2 editing ncRNA and gRNA
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1: RNF2 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    Tags
    SV40NLS
    Expression
    Mammalian
    Mutation
    RNF2 donor, a1/a2 length extended for 27 bp
    Promoter
    H1
    Available Since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    184997-plasmid-map-sequence-id-376646

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  12. pSCL.179

    Plasmid
    #185000
    Purpose
    Express -Eco1 HEK4 editing ncRNA and gRNA
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1: HEK4 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    Tags
    SV40NLS
    Expression
    Mammalian
    Mutation
    HEK4 donor, a1/a2 length extended for 27 bp
    Promoter
    H1
    Available Since
    Nov. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    185000-plasmid-map-sequence-id-376622

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  19. pH-ABE8e

    Plasmid
    #200774
    Purpose
    For plant adenine base editing in rice plants or monocotyledons protoplasts
    Depositor
    Caixia Gao
    Article
    Xue et al Nat Biotechnol. 2023 Mar 9. doi: 10.1038
    Insert
    nCas9(D10A)-TadA8e
    Use
    CRISPR
    Expression
    Plant
    Mutation
    D10A for Cas9; W23R, H36L, P48A, R51L, L84F, A106…
    Promoter
    maize Ubiquitin-1, OsU3
    Available Since
    July 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    200774-plasmid-map-sequence-id-399597

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Showing: 6201 - 6220 of 6593 results