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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,562 results for: nls

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Showing: 6201 - 6220 of 6562 results

  1. pPD50_14

    Plasmid
    #1449
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    hsp16-415', hsp16-25', IVSA, SV40NLS, l…
    Expression
    Worm
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1449-plasmid-map-sequence-id-60

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  2. L2963

    Plasmid
    #1528
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    SV40NLS, gfpN, IVSR, gfpS65C, IVSS, IVST, IVS
    Expression
    Worm
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1528-plasmid-map-sequence-id-138

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  5. pPD91_14

    Plasmid
    #1472
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    unc54-204, unc545', IVSA, SV40NLS, gfpN, gfp…
    Expression
    Worm
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1472-plasmid-map-sequence-id-83

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  7. pPD93_51

    Plasmid
    #1474
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    unc54-204, unc545', IVSA, SV40NLS, gfpN, IVS…
    Expression
    Worm
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1474-plasmid-map-sequence-id-85

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  8. L2684

    Plasmid
    #1519
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    hsp16-25', hsp16-415', IVSA, SV40NLS, g…
    Expression
    Worm
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1519-plasmid-map-sequence-id-129

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  10. L4632

    Plasmid
    #1661
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    SV40NLS, gfpN, IVSR, gfpGLA, IVSS, IVST, gfpC, IV…
    Expression
    Worm
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1661-plasmid-map-sequence-id-271

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  11. pJKL464

    Plasmid
    #1689
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    hlh8-280, IVSA, rf2204, SV40NLS, gfpN, IVSR, gfpS…
    Expression
    Worm
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1689-plasmid-map-sequence-id-299

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  12. Unfused click editor construct; mSA-EcKlenow fusion - pCMV-T7-mSA-EcKlenow (JO1401)

    Plasmid
    #217806
    Purpose
    Unfused click editor construct expressing mSA-EcKlenow, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Publication
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    mSA-linker-SV40NLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217806-plasmid-map-sequence-id-432904

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  13. AA058

    Plasmid
    #216040
    Purpose
    Fragmid fragment: (C' terminus) for prime editing
    Depositor
    John Doench
    Publication
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MMLV-RT_v1.1; SV40NLS_v1.3
    Use
    Fragment
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216040-plasmid-map-sequence-id-431496

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  14. pQP-NiRISB

    Plasmid
    #138977
    Purpose
    Light-controlled tri-directional targeting of endogenous proteins (nucleus-shifted)
    Depositor
    Vladislav Verkhusha
    Publication
    Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NcoI-BphP1-CAAX-IRES2-NES- iB(GFP)- mCherry-Q-PAS1-AsLOV2cNLS-NotI
    Available since
    April 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    138977-plasmid-map-sequence-id-292251

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  15. pPD95_07

    Plasmid
    #1480
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    IVSA, rf2211, SV40NLS, lacZN, IVSB, IVSD, IVSE, I…
    Expression
    Worm
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1480-plasmid-map-sequence-id-91

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  16. pPD95_10

    Plasmid
    #1481
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    rf8134, IVSA, SV40NLS, lacZN, IVSB, IVSD, IVSE, I…
    Expression
    Worm
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    1481-plasmid-map-sequence-id-92

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  17. L4458

    Plasmid
    #1657
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    unc54-204, unc545', IVSA, SV40NLS, gfpN, IVS…
    Expression
    Worm
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1657-plasmid-map-sequence-id-267

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  18. C-term AAV-eVRQR-ACTA2-gRNA-A4 (mouse) (LLH310)

    Plasmid
    #242662
    Purpose
    CBh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpCas9-VRQR and mouse gRNA-A4
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pAAV-pCbh-BPNLS-NpuC-SpCas9-VRQR-BPNLS-WPRE-bGH_PA-ACTA2_mouse_NGA_gRNA-pU6
    Use
    AAV and CRISPR
    Tags
    BPNLS
    Mutation
    VRQR mutations in SpCas9(S55R/D1135V/G1218R/R1335…
    Promoter
    CBh
    Available since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242662-plasmid-map-sequence-id-489091

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  19. C-term AAV-eVRQR-ACTA2-gRNA-A4 (human) (LLH301)

    Plasmid
    #242661
    Purpose
    CBh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpCas9-VRQR and human gRNA-A4
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pAAV-pCbh-BPNLS-NpuC-SpCas9-VRQR-BPNLS-WPRE-bGH_PA-ACTA2_human_NGA_gRNA-pU6
    Use
    AAV and CRISPR
    Tags
    BPNLS
    Mutation
    VRQR mutations in SpCas9(S55R/D1135V/G1218R/R1335…
    Promoter
    CBh
    Available since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242661-plasmid-map-sequence-id-489090

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  20. HUHe variant click editor (CE1) - pCMV-T7-StCV-nCas9-EcKlenow (JO1269)

    Plasmid
    #217790
    Purpose
    Variant CE1 construct with StCV HUH endonuclease, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Publication
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    StCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217790-plasmid-map-sequence-id-432853

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Showing: 6201 - 6220 of 6562 results