We narrowed to 8,829 results for: sgRNA

1. pEPOR1CB0089

   Plasmid

   #117565

   Purpose

   Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting {AT1G68250} in A. thaliana

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   Promoter_AtU6-26 + sgRNA_{AT1G68250} A. thaliana
   

   Expression

   Plant

   Promoter

   AtU6-26 (pICSL90002)

   Available Since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEPOR1CB0087

   Plasmid

   #117564

   Purpose

   Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting AtUUAT1 {AT5G04160} in A. thaliana

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   Promoter_AtU6-26 + sa_gRNA_UUAT1 {AT5G04160} A. thaliana
   

   Expression

   Plant

   Promoter

   AtU6-26 (pICSL90002)

   Available Since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEPOR1CB0084

   Plasmid

   #117563

   Purpose

   Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting AtCPK34 {AT5G19360} in A. thaliana

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   Promoter_AtU6-26 + sgRNA_AtCPK34 {AT5G19360} A. thaliana
   

   Expression

   Plant

   Promoter

   AtU6-26 (pICSL90002)

   Available Since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEPOR1CB0083

   Plasmid

   #117562

   Purpose

   Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting Methyltransferase {AT5G10620} in A. thaliana

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   Promoter_AtU6-26 + sgRNA_Methyltransferase {AT5G10620} A. thaliana
   

   Expression

   Plant

   Promoter

   AtU6-26 (pICSL90002)

   Available Since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEPOR1CB0081

   Plasmid

   #117561

   Purpose

   Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting AtMPK19 {AT3G14720} in A. thaliana

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   Promoter_AtU6-26 + sgRNA_AtMPK19 {AT3G14720} A. thaliana
   

   Expression

   Plant

   Promoter

   AtU6-26 (pICSL90002)

   Available Since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEPOR1CB0114

   Plasmid

   #117553

   Purpose

   Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting NbPDS gene in N. benthamiana

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   Promoter_AtU6-26 + sgRNA_NbPDS1 N. benthamiana
   

   Expression

   Plant

   Promoter

   At U6-26 (pICSL90002)

   Available Since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEPOR1CB0029

   Plasmid

   #117541

   Purpose

   Level1 Golden Gate Cassette: sgRNA cassette for LbCas12a, targeting NbPDS gene in N. benthamiana

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   crRNA (LbCas12a)
   

   Expression

   Plant

   Available Since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEPOR1CB0028

   Plasmid

   #117540

   Purpose

   Level1 Golden Gate Cassette: sgRNA cassette for AsCas12a, targeting NbPDS gene in N. benthamiana

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   crRNA (AsCas12a)
   

   Expression

   Plant

   Available Since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEPOR2KN0049

   Plasmid

   #117616

   Purpose

   Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_AtMPK19 {AT3G14720}(pEPOR1CB0081)

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   [35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]
   

   Expression

   Plant

   Available Since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-hUbC-GBX2-CDX124-Cas9-T2A-GFP

    Plasmid

    #192287

    Purpose

    Expresses Cas9-T2A-GFP and four sgRNAs: GBX2, CDX1, CDX2, CDX4

    Depositor

    Mark Denham

    Article

    Maimaitili et al Nat Commun. 2023 Dec 5;14(1):7871. doi:

    Insert

    Cas9-T2A-GFP and four sgRNA: GBX2 + CDX1 + CDX2 + CDX4 (GBX2, CDX2, CDX1, CDX4 Synthetic, Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBS-GBX2-CDX124-Ef1a-mCherry-Neo

    Plasmid

    #192823

    Purpose

    Expresses mCherry and four sgRNAs: GBX2, CDX1, CDX2, CDX4

    Depositor

    Mark Denham

    Article

    Maimaitili et al Nat Commun. 2023 Dec 5;14(1):7871. doi:

    Insert

    mCherry and sgRNA CDX1 CDX2 CDX4 and GBX2 (CDX2, CDX1, CDX4, GBX2 Synthetic)
    

    Use

    CRISPR and Cre/Lox

    Expression

    Mammalian

    Available Since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. AIO-mCherry

    Plasmid

    #74120

    Purpose

    All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and mCherry-coupled Cas9-D10A nickase to enhance efficient and accurate genome editing

    Depositor

    Steve Jackson

    Article

    Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    May 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAID-EF1a linearizing in pX330

    Plasmid

    #140610

    Purpose

    Crispr/Cas9 plasmid for linearization of pAID-EF1a

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    sgRNA for pAID-EF1a plasmid digestion
    

    Use

    CRISPR

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. PB_rtTA_BsmBI

    Plasmid

    #126028

    Purpose

    PiggyBac cargo vector expressing rtTA, contains BsmBI sites for sgRNA cloning

    Depositor

    Mauro Calabrese

    Article

    Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

    Type

    Empty backbone

    Use

    CRISPR; Piggybac

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. AIO-GFP

    Plasmid

    #74119

    Purpose

    All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and EGFP-coupled Cas9-D10A nickase to enhance efficient and accurate genome editing

    Depositor

    Steve Jackson

    Article

    Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    May 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEvolvR-enCas9-PolI3M-TBD

    Plasmid

    #113077

    Purpose

    Expresses enCas9 fused to PolI3M-TBD in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

    Depositor

    John Dueber, David Schaffer

    Article

    Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

    Insert

    enCas9-PolI3M-TBD
    

    Expression

    Bacterial

    Available Since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. 2X_pX458_pSpCas9(BB)-2A-GFP

    Plasmid

    #172221

    Purpose

    Cas9-2A-GFP expression vector bearing two (one additional) independent sgRNA cassettes.

    Depositor

    Alexander Meissner

    Article

    Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCBEclos-cbei2385-g2-opt

    Plasmid

    #118215

    Purpose

    CRISPR-mediated base editing in Clostridium

    Depositor

    Sheng Yang

    Article

    Li et al Biotechnol Bioeng. 2019 Feb 10. doi: 10.

    Insert

    Apobec1, Casp nickase, UG1-sgRNA
    

    Expression

    Bacterial

    Promoter

    Ptm

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSGAb-km

    Plasmid

    #121999

    Purpose

    A sgRNA expression plasmid for genome editing in Acinetobacter baumannii

    Depositor

    Quanjiang Ji

    Article

    Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

    Insert

    none
    

    Use

    CRISPR

    Available Since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pgRNAtet-lvaA

    Plasmid

    #106397

    Purpose

    Guide RNA plasmid targeting lvaA on BBR1-UP origin

    Depositor

    Brian Pfleger

    Article

    Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

    Insert

    sgRNA towards lvaA in Pseudomonas putida
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    March 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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