We narrowed to 8,829 results for: sgRNA

1. pCyCBE

   Plasmid

   #226505

   Purpose

   A cytidine deaminase-mediated base-editing plasmid in cyanobacteria

   Depositor

   Weizhong Chen

   Article

   Li et al Commun Biol. 2024 Aug 14;7(1):994. doi:

   Inserts

   APOBEC1-Cas9D10A
   sgRNA
   sacB gene
   

   Use

   CRISPR

   Available Since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEvolvR-enCas9-PolI5M

   Plasmid

   #113078

   Purpose

   Expresses enCas9 fused to PolI5M in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

   Depositor

   John Dueber, David Schaffer

   Article

   Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

   Insert

   enCas9-PolI5M
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDNR-gRNA

   Plasmid

   #206990

   Purpose

   A plasmid for expression of SaCas9-sgRNA targeting donor plasmids

   Depositor

   Alejandro Chavez

   Article

   Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi

   Insert

   Donor plasmid-targeting SaCas9-gRNA
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSGAb-spe

   Plasmid

   #122000

   Purpose

   A sgRNA expression plasmid for genome editing in Acinetobacter baumannii

   Depositor

   Quanjiang Ji

   Article

   Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

   Insert

   none
   

   Use

   CRISPR

   Available Since

   Sept. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCT-tRNA

   Plasmid

   #133813

   Purpose

   CRISPR-Cas9 system for genetic manipulation of Candida tropicalis

   Depositor

   Geraldine Butler

   Article

   Lombardi et al mSphere. 2019 Mar 13;4(2). pii: 4/2/e001

   Insert

   cassette for the expression of the sgRNA from the Ashbya gossypii RNA pol II TEF1 promoter
   

   Use

   CRISPR

   Available Since

   Dec. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. lentiCRISPR v2-sgUpf1

   Plasmid

   #161810

   Purpose

   guide RNA for Upf1

   Depositor

   Yi Zhang

   Article

   Fu et al Sci Adv. 2020 May 29;6(22):eaay5181. doi

   Insert

   sgRNA targeting mouse Upf1 (Upf1 Mouse, Synthetic)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQdCas9-sgempty

   Plasmid

   #159523

   Purpose

   Expression of dCas9 driven by lux cassette from Vibrio fischeri

   Depositor

   Radhakrishnan Mahadevan

   Article

   Liu et al ACS Synth Biol. 2020 Jun 5. doi: 10.1021

   Insert

   quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dcas9 and sgRNA
   

   Use

   CRISPR

   Promoter

   quorum sensing promoter

   Available Since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLentiCRISPR V2 sgIREB2_2

   Plasmid

   #169886

   Purpose

   Disrupt IREB2

   Depositor

   Richard Possemato

   Article

   Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

   Insert

   sgRNA targeting IREB2
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. KO plasmid

   Plasmid

   #135970

   Purpose

   Expresses SpCas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Wang et al J Biol Eng. 2019 Apr 24;13:35. doi: 10.1

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. px459 VQR

    Plasmid

    #101715

    Purpose

    sgRNA/Cas9 expression plasmid with Cas9 VQR mutations (NGA PAM)

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VQR
    

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VQR (D1135V, R1335Q and T1337R)

    Available Since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. PJQ49

    Plasmid

    #134657

    Purpose

    Contains Promoter 3 with bsa1 cut sites for inserting sgRNA

    Depositor

    Oren Rosenberg, Jason Peters

    Article

    Qu et al J Bacteriol. 2019 Sep 3. pii: JB.00304-1

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    Anderson BBa_J23115

    Available Since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. PJQ47

    Plasmid

    #134646

    Purpose

    Contains Promoter 1 with bsa1 cut sites for inserting sgRNA

    Depositor

    Oren Rosenberg, Jason Peters

    Article

    Qu et al J Bacteriol. 2019 Sep 3. pii: JB.00304-1

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    Anderson BBa_J23117

    Available Since

    Feb. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. PJQ48

    Plasmid

    #134647

    Purpose

    Contains Promoter 2 with bsa1 cut sites for inserting sgRNA

    Depositor

    Oren Rosenberg, Jason Peters

    Article

    Qu et al J Bacteriol. 2019 Sep 3. pii: JB.00304-1

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    Anderson BBa_J23114

    Available Since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAWp28

    Plasmid

    #73850

    Purpose

    pBT264-U6p-{2xBbsI}-sgRNA scaffold-{MfeI}

    Depositor

    Timothy Lu

    Article

    Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

    Type

    Empty backbone

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAUX_OL

    Plasmid

    #166246

    Purpose

    use as an auxiliary temperature-sensitive plasmid to successfully transform pdCas9_CL plasmid

    Depositor

    Domitilla Del Vecchio

    Article

    Huang et al Nat Commun. 2021 Mar 16;12(1):1692. doi:

    Insert

    [P112-sgRNA-term]-[J23116_B34-dCas9-B15]
    

    Expression

    Bacterial

    Promoter

    Ec-TTL-P112 and BBa_J23116

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBLO1811_arC9_noNLS_human

    Plasmid

    #74494

    Purpose

    Human codon optimized plasmid to express arC9 t2a mCherry w/o NLS and a sgRNA

    Depositor

    David Savage

    Article

    Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Insert

    arC9
    

    Use

    CRISPR

    Tags

    t2a mCherry (C-terminal on insert)

    Expression

    Mammalian

    Available Since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAbaMCi-GFP-BsaI_pJMP2748

    Plasmid

    #208888

    Purpose

    Acinetobacter baumannii CRISPRi test plasmid (GFP, BsaI cloning site in gRNA cassette).

    Depositor

    Jason Peters

    Article

    Ward et al mBio. 2023 Dec 21:e0205123. doi: 10.1128

    Insert

    dCas9, sgRNA expression cassette, sfGFP
    

    Use

    Pir dependent origin of replication

    Expression

    Bacterial

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSK-Sasg

    Plasmid

    #214350

    Purpose

    Expresses cloning backbone for SlugCas9 sgRNA

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SlugCas9 tracrRNA
    

    Expression

    Mammalian

    Available Since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV-sgTel

    Plasmid

    #122659

    Purpose

    Expresses sgRNA for telomere repeats with fluorescent indicator BFP and puromycin selection marker

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

    Insert

    BFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTJV1Sc3-rpoB

    Plasmid

    #166983

    Purpose

    Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ EcRecT recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance

    Depositor

    Christopher Reisch

    Article

    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

    Insert

    RecT (recT E. coli)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    pVvanCC

    Available Since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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