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SG3ΔENV CA M66I

Plasmid

#149687

Purpose

Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and M66I mutation in capsid (CA M66I).

Depositor

Wesley Sundquist

Article

Christensen et al Science. 2020 Oct 9;370(6513). pii: 370/

Insert

Capsid

Use

Lentiviral and Retroviral

Expression

Mammalian

Mutation

Changed Capsid residue Met 66 to Iso (CA M66I)

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA_GFP-mTLN1-FL

Plasmid

#164838

Purpose

Expression of GFP tagged FL mouse talin1

Depositor

Tina Izard

Article

Rangarajan et al J Biol Chem. 2020 Sep 11;295(37):12885-1

Insert

TLN1 (Tln1 Mouse)

Tags

His10-GFP

Expression

Mammalian

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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p3E-ML1N*2

Plasmid

#67678

Purpose

Multisite gateway entry clone for adding C-terminal fusions of ML1N*2

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

ML1N*2

Use

Gateway multisite 3' entry clone

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only

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EFF-1::V5

Plasmid

#132961

Purpose

Expresses EFF-1 A full length protein in mammalian cells

Depositor

Benjamin Podbilewicz

Article

Valansi et al J Cell Biol. 2017 Mar 6;216(3):571-581.

Insert

EFF-1::V5

Tags

V5

Expression

Mammalian

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pME-APEX2-NS

Plasmid

#67669

Purpose

Gateway entry clone containing APEX2 with no stop codon

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

APEX2

Use

Gateway multisite middle entry clone

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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mCherry-miniSOG-N1

Plasmid

#54803

Purpose

Localization: N1 Cloning Vector, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Type

Empty backbone

Tags

mCherry-miniSOG

Expression

Mammalian

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only
mc2 puro EF1a

Plasmid

#206221

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pSR49

Plasmid

#69259

Purpose

phlh-8::tagBFP2

Depositor

Sander van den Heuvel

Article

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Insert

TagBFP2

Use

Cre/Lox

Expression

Worm

Mutation

codon-optimzed index 1.0, TagBFP2 contains 2 sing…

Promoter

hlh-8

Available Since

Oct. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pEF-pIgH-ESCargo*

Plasmid

#140159

Purpose

Expresses a regulatable secretory cargo (without an ER export signal) for mammalian cells

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

piGH-DsRed-Express2-FKBP(LV)(C22V)

Tags

ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

Expression

Mammalian

Promoter

EF-1a

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1354

Plasmid

#69490

Purpose

Gly-Ser-4xGly linker-degron-TEV-3xFLAG with 40 bp nhr-25 homology arms

Depositor

Jordan Ward

Article

Zhang et al Development. 2015 Nov 9. pii: dev.129635

Insert

GSGGGG spacer-Degron-TEV protease site-3x FLAG epitope

Use

CRISPR; Template to generate cassettes for -media…

Available Since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pHCMM4

Plasmid

#170359

Purpose

Expresses a Sec61p-mCherry ER-marker from the CYC1 promoter

Depositor

Hugo Cesar Medina-Munoz, Marvin Wickens

Article

Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

PCYC1_SEC61_mCherry_LEU2_TCYC1

Expression

Yeast

Promoter

PCYC1

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pHH0103_RBM17_RRM

Plasmid

#109786

Purpose

Protein expression and purification of RBM17_RRM

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

RBM17_RRM (RBM17 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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untargeted Plk1 FRET sensor Myt1 substrate

Plasmid

#45217

Depositor

Michael Lampson

Article

Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

Insert

untargeted Plk1 FRET sensor Myt1 substrate

Expression

Mammalian

Promoter

t7

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-LifeAct-Venus

Plasmid

#87613

Purpose

Actin-targeted Venus (YFP).

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

LifeAct-Venus

Tags

17-aa sequence from Abp140 (aka "Lifeact&quo…

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_NR1D1_Hormone-recep

Plasmid

#109960

Purpose

Protein expression and purification of NR1D1_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

NR1D1_Hormone-recep

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pME-H2B-mCerulean3

Plasmid

#75156

Purpose

Nuclear mCerulean2 (blue) fluorophore for use with 5’ promoters.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

H2B-mCerulean3

Use

Middle entry vector for multisite gateway three f…

Promoter

n/a

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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CENP-B targeted Aurora B FRET sensor Kif2 substrate

Plasmid

#45214

Depositor

Michael Lampson

Article

Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

Insert

CENP-B targeted Aurora B FRET sensor

Expression

Mammalian

Promoter

t7

Available Since

May 30, 2013

Availability

Academic Institutions and Nonprofits only

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pET30a-FluB-NP

Plasmid

#165093

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Article

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Influenza B virus(B/Georgia/05/2019) nucleocapsid protein

Expression

Bacterial

Available Since

Feb. 11, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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myc-Orai1 R91W MO93

Plasmid

#22756

Depositor

Anjana Rao

Article

Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

Insert

Orai1 R91W (ORAI1 Human)

Tags

myc

Expression

Mammalian

Mutation

R91W

Available Since

March 15, 2012

Availability

Academic Institutions and Nonprofits only
pGrDL_SP

Plasmid

#83204

Purpose

receives miRNA target sequences for testing using the dual luciferase assay system

Depositor

Peer Schenk

Article

Moyle et al Front Plant Sci. 2017 Sep 20;8:1631. doi

Insert

SalI/PstI cloning sites

Expression

Plant

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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