161,377 results

1. pSOUP

   Plasmid

   #165419

   Purpose

   The pSOUP helper plasmid needed for replication of all pGreen (Hellens et al., 2000) based vectors in Agrobacterium

   Depositor

   John Innes Centre - Wheat Transformation and Genome Editing, Wendy Harwood

   Article

   Hayta et al Curr Protoc. 2021 Mar;1(3):e58. doi: 10.

   Insert

   Rep A
   

   Use

   Helper plasmid required for replication for all p…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR223_BFP

   Plasmid

   #25891

   Depositor

   David Root

   Article

   Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

   Insert

   BFP
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR_PGK

   Plasmid

   #79120

   Purpose

   Lentiviral vector for constitutive expression of genes (PGK promoter)

   Depositor

   Wendell Lim

   Article

   Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCR1316

   Plasmid

   #111126

   Purpose

   Expresses FANCA.

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

   Insert

   FANCA (FANCA Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available since

   Oct. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMRX-IP-TMEM192-mKeima

   Plasmid

   #215358

   Purpose

   Probe to evaluate lysophagic activity

   Depositor

   Tamotsu Yoshimori

   Article

   Shima et al J Cell Biol. 2023 Dec 4;222(12):e2022040

   Insert

   transmembrane protein 192 (TMEM192 Human)
   

   Use

   Retroviral

   Tags

   mKeima

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-X1-Neo-DDRGK1-WT-HA

   Plasmid

   #139845

   Purpose

   Lentiviral expression of human DDRGK1-WT-HA

   Depositor

   Jacob Corn

   Article

   Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

   Insert

   DDRGK1 (DDRGK1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available since

   July 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR-SFFV-KRAB-dCas9-P2A-mCherry

   Plasmid

   #60954

   Purpose

   2nd Generation Lentiviral vector. Expresses an N-terminal KRAB-dCas9 fusion protein and mCherry

   Depositor

   Jonathan Weissman

   Article

   Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

   Insert

   KRAB-dCas9-P2A-mCherry fusion
   

   Use

   CRISPR and Lentiviral

   Tags

   2x NLS, HA, KRAB domain, and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Jan. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. FAS_pLX307

   Plasmid

   #98334

   Purpose

   Lentiviral expression of FAS

   Depositor

   William Hahn, Sefi Rosenbluh

   Article

   Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

   Insert

   FAS (FAS Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   E1Fa

   Available since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCWori cytochrome P450-BM3h 9D7

   Plasmid

   #61308

   Purpose

   Expresses Cytochrome P450-BM3h 9D7 with C-terminal 6-His tag

   Depositor

   Alan Jasanoff

   Article

   Brustad et al J Mol Biol. 2012 Sep 14;422(2):245-62. d

   Insert

   Cytochrome P450-BM3 heme domain 9D7
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   T268A, I263A, T438V, A328G

   Promoter

   Tac

   Available since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pME-nfsB-CFP

    Plasmid

    #86374

    Purpose

    expression of nfsB-CFP

    Depositor

    Rachel Wong

    Article

    Yoshimatsu et al Nat Commun. 2016 Feb 3;7:10590. doi: 10.

    Insert

    nitroreductase
    

    Use

    Zebrafish

    Tags

    ECFP

    Expression

    Mutation

    Promoter

    Available since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLminP_Luc2P_RE26

    Plasmid

    #90368

    Purpose

    C/EBPb - CEBPB gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CEBPb

    Available since

    Aug. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMOS007: PercevalHR ATP/ADP sensor (cytosolic)

    Plasmid

    #163061

    Purpose

    PercevalHR ATP/ADP sensor (cytosolic)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    PercevalHR
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pATZ-Myc

    Plasmid

    #177718

    Purpose

    ER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant), myc tagged

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    alpha-1-antitrypsin (SERPINA1 Human)
    

    Use

    Tags

    Myc

    Expression

    Bacterial and Mammalian

    Mutation

    E342K (ATZ mutant)

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. Tet1-dCas9

    Plasmid

    #136650

    Purpose

    Tet1 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

    Depositor

    David Segal

    Article

    Umeh-Garcia et al Br J Cancer. 2022 Apr 19. pii: 10.1038/s

    Insert

    Tet1 (Tet1 Mouse)
    

    Use

    CRISPR

    Tags

    3XFLag-NLS-Tet1-dCas9-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRS410

    Plasmid

    #11258

    Depositor

    Fred Cross

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. EYFP-JAB1

    Plasmid

    #111213

    Purpose

    fluorescent fusion protein

    Depositor

    Johannes A. Schmid

    Article

    Orel et al J Cell Mol Med. 2010 Jun;14(6B):1555-68.

    Insert

    CSN5 (COPS5 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. mT-Sapphire-N1

    Plasmid

    #54569

    Purpose

    Localization: N1 Cloning Vector, Excitation: 399, Emission: 511

    Depositor

    Michael Davidson, Robert Campbell

    Article

    Ai et al Nat Methods. 2008 May . 5(5):401-3.

    Type

    Empty backbone

    Use

    Tags

    mT

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. piggyBac-ABE8.20-NG

    Plasmid

    #226866

    Purpose

    Expresses dox-inducible ABE8.20-NG for mammalian cells. piggyBac vector

    Depositor

    Alexis Komor

    Article

    Osgood et al Methods in Enzymology, February 5, 2025

    Insert

    ABE8.20-NG
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Tet-On-TRE3G

    Available since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV-dCas9-p300-P2A-PuroR

    Plasmid

    #83889

    Purpose

    Lentiviral Sp dCas9-p300-P2A-PuroR

    Depositor

    Charles Gersbach

    Article

    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

    Inserts

    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, S. pyogenes, Human)
    pac from Streptomyces alboniger
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag and nucleoplasmin NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EFS

    Available since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTALYM3B15

    Plasmid

    #47878

    Purpose

    TALE-mClover against mouse major satellite sequence

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Miyanari et al Nat Struct Mol Biol. 2013 Oct 6. doi: 10

    Insert

    TALE-mClover
    

    Use

    TALEN

    Tags

    mClover

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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