163,977 results

1. pAAV-CW3SL-EGFP

   Plasmid

   #61463

   Purpose

   AAV vector with CaMKIIa promoter, EGFP, and W3SL(shorten expression cassette with relatively high expression to maximize expression of larger genes)

   Depositor

   Bong-Kiun Kaang

   Article

   Choi et al Mol Brain. 2014 Mar 11;7:17. doi: 10.118

   Insert

   EGFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CaMKIIa

   Available since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. Mouse Cherry Brie Pooled Library

   Pooled Library

   #170511

   Purpose

   CRISPR library targeting mouse genes

   Depositor

   Pavel Tolar

   Article

   Newman et al biorXiv 2021.03.02.433398

   Expression

   Mammalian

   Species

   Synthetic

   Use

   CRISPR and Lentiviral

   Available since

   Sept. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

3. pSecUAG-Evol2

   Plasmid

   #163148

   Purpose

   Expresses machinery necessary for selenocysteine incorporation at UAG codons

   Depositor

   Dieter Söll

   Article

   Mukai et al Angew Chem Int Ed Engl. 2018 Jun 11;57(2

   Inserts

   allo-tRNA UTu2D
   thioredoxin
   Selenophosphate synthase
   Selenocysteine synthase
   Cysteine desulfurase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Cysteine 32 switched to Selenocysteine (UAG), Cys…

   Promoter

   EM7, araBAD, native As SelD promoter, and native …

   Available since

   April 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDR121

   Plasmid

   #37148

   Depositor

   Dale Ramsden

   Insert

   XRCC6 (XRCC6 Human)
   

   Use

   Tags

   H6

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 18, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pLV-dCas9-p300-P2A-PuroR

   Plasmid

   #83889

   Purpose

   Lentiviral Sp dCas9-p300-P2A-PuroR

   Depositor

   Charles Gersbach

   Article

   Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

   Inserts

   S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, S. pyogenes, Human)
   pac from Streptomyces alboniger
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag and nucleoplasmin NLS

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EFS

   Available since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSBbi G418R mbIL21

   Plasmid

   #194329

   Purpose

   Consitiutive mammalian expression of membrane bound human IL-21

   Depositor

   Alexander McLellan

   Article

   Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

   Insert

   mbIL-21 (IL21 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-a

   Available since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-MSP1D1delltaH5

   Plasmid

   #71714

   Purpose

   Membrane scaffold protein (MSP), helix 5 deletion

   Depositor

   Gerhard Wagner

   Article

   Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

   Insert

   Apolipoprotein A1 (APOA1 Human)
   

   Use

   Tags

   6xHis and TEV site

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDR123

   Plasmid

   #37149

   Depositor

   Dale Ramsden

   Insert

   XRCC5 (XRCC5 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 18, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pLV.PGK.mFoxa2

   Plasmid

   #33014

   Depositor

   Malin Parmar

   Article

   Pfisterer et al Proc Natl Acad Sci U S A. 2011 Jun 21;10

   Insert

   Forkhead box A2 (Foxa2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   H10L

   Promoter

   Available since

   Jan. 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. GPI-mCherry2

    Plasmid

    #182869

    Purpose

    Monomer expression vector with plasma membrane localization signal

    Depositor

    Salvatore Chiantia

    Article

    Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

    Insert

    GPI-mCherry2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1/CALR wt-FLAG

    Plasmid

    #204532

    Purpose

    Mammalian expression of human CALR wt-FLAG

    Depositor

    Norio Komatsu

    Article

    Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

    Insert

    CALR wt-FLAG (CALR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. MSCV-IRES-Thy1.1 DEST

    Plasmid

    #17442

    Purpose

    Destination vector derived from MSCV-IRES-Thy1.1. Please note that this plasmid contains attB1 and attB2 sites for use in a BP reaction

    Depositor

    Anjana Rao

    Article

    Wu et al Cell. 2006 Jul 28. 126(2):375-87.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSBbi-Pur

    Plasmid

    #60523

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: puromycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLPCX cyto Grx1-roGFP2

    Plasmid

    #64975

    Purpose

    Mammalian expression of cytosolic Grx1-roGFP2 (retroviral vector)

    Depositor

    Tobias Dick

    Article

    Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

    Insert

    Glutaredoxin-1 (GLRX Human)
    

    Use

    Retroviral

    Tags

    roGFP2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEvolvR-enCas9-PolI5M

    Plasmid

    #113078

    Purpose

    Expresses enCas9 fused to PolI5M in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

    Depositor

    John Dueber, David Schaffer

    Article

    Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

    Insert

    enCas9-PolI5M
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. AbVec2.0-mIgkc

    Plasmid

    #127157

    Purpose

    Expression of immunoglobulin light chains in mammalian cells, mouse (C57BL/6), kappa constant region

    Depositor

    Hedda Wardemann

    Article

    Lorenzo et al Nature. 2021 Jan;589(7841):287-292. doi:

    Insert

    Igkc (Igkc Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV IE1 gene promoter

    Available since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-R2Tg_opt-3xFlag

    Plasmid

    #223251

    Purpose

    Mammalian expression of C-terminally Flag-tagged optimized R2Tg protein

    Depositor

    Wei Li

    Article

    Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

    Insert

    R2Tg protein_opt
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. mVenus C1

    Plasmid

    #27794

    Depositor

    Steven Vogel

    Article

    Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

    Has Service

    Cloning Grade DNA

    Insert

    mVenus C1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP-TDP-43

    Plasmid

    #235487

    Purpose

    Expression of human TDP-43-EGFP

    Depositor

    Maxime Rousseaux

    Article

    Suk et al Mol Neurodegener. 2025 Mar 28;20(1):38.

    Insert

    TARDBP (TARDBP Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. L4440_BioBrick-sgRNA

    Plasmid

    #177783

    Purpose

    Overexpression of sgRNAs in E. coli HT115, sgRNA overexpression scaffold; for ingestion by C. elegans

    Depositor

    Michael Ristow

    Article

    Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge