163,983 results

1. pABY-T2

   Plasmid

   #201765

   Purpose

   For C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeast

   Depositor

   Saravanan Palani

   Article

   Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

   Insert

   ALFA tag
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCX137

   Plasmid

   #229212

   Purpose

   CRISPR array plasmid for NOTCH2 (Spacers 1-24)

   Depositor

   Jennifer Doudna

   Article

   Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

   Insert

   CRISPR array plasmid for NOTCH2 (Spacers 1-24)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato (AAV1)

   Viral Prep

   #51082-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from AAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato (#51082). In addition to the viral particles, you will also receive purified AAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato plasmid DNA. GCaMP6s calcium sensor and bicistronic, physically separate nuclear localized dTomato expression under the EF1a promoter. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Jonathan Ting

   Promoter

   EF1a

   Tags

   nuclear dTomato (physically separate, not a fusion protein)

   Available since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

4. pEGFP-N1-FUS/TLS-FLAGC

   Plasmid

   #60362

   Purpose

   Plasmid for expression of FLAG-GFP tagged human FUS/TLS (C-terminal tag). Confers resistance to G418.

   Depositor

   Sébastien Britton, Patrick Calsou

   Article

   Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

   Insert

   FUS (FUS Human)
   

   Use

   Tags

   FLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCre

   Plasmid

   #236212

   Purpose

   Arabinose-inducible Cre recombinase

   Depositor

   Matthew Waldor

   Article

   Basta et al Nat Microbiol. 2025 Mar 27. doi: 10.1038

   Insert

   Cre recombinase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. mApple-LAMP1-phLuorin-N-8

   Plasmid

   #54918

   Purpose

   Localization: Lysosome Membrane, Excitation: 568, Emission: 592

   Depositor

   Michael Davidson

   Insert

   LAMP1 phLuorin
   

   Use

   Tags

   mApple

   Expression

   Mammalian

   Mutation

   Lysosomes-20 double labeled with phLuorin

   Promoter

   CMV

   Available since

   Aug. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. AiP13583 - pAAV-AiE0475m_3xC1-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3583)

   Plasmid

   #224030

   Purpose

   AiE0475m_3xC1 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Pvalb+ neurons (sparse ChC cells)

   Depositor

   Jonathan Ting, The Allen Institute for Brain Science

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   minBG

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. AiP13584 - pAAV-AiE0475m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3584)

   Plasmid

   #224031

   Purpose

   AiE0475m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Chandelier cells

   Depositor

   Jonathan Ting, The Allen Institute for Brain Science

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   minBG

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-Pom121 GFP

   Plasmid

   #187680

   Purpose

   In vivo visualization of NPC protein Pom121

   Depositor

   Mary Beckerle

   Insert

   Pom121 (Pom121 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLminP_Luc2P_RE46

    Plasmid

    #90384

    Purpose

    NFYA - constitutive enhancer gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EGR1

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGL2-GAL4-UAS-Luc

    Plasmid

    #33020

    Depositor

    Martin Walsh

    Article

    Nishio et al Proc Natl Acad Sci U S A. 2004 Aug 3;101

    Insert

    6X GAL4-UAS
    

    Use

    Tags

    Luciferase

    Expression

    Mutation

    Promoter

    Available since

    April 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-hSyn-miniDi-P2A-mCherry

    Plasmid

    #204358

    Purpose

    AAV vector for miniDi expression under the control of human synapsin promoter

    Depositor

    Masao Doi

    Article

    Miyake et al Cell Rep Methods. 2024 Oct 21;4(10):1008

    Insert

    miniDi-P2A-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    The third intracellular loop (ICL3) of hM4Di was …

    Promoter

    hSyn

    Available since

    Oct. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAS9-mCherry-Frame +2

    Plasmid

    #66941

    Purpose

    CRISPaint frame selector +2

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    gRNA frame +2
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti PGK V5-LUC Puro (w543-1)

    Plasmid

    #19360

    Purpose

    3rd gen lentiviral V5-Luciferase expression vector, PGK promoter, Puro

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    Firefly Luciferase
    

    Use

    Lentiviral and Luciferase

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1 TaGu N-1F

    Plasmid

    #203762

    Purpose

    For expression of N-flag tagged TaGu R2 protein in mammalian cells

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    R2 retroelement protein
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBABE

    Plasmid

    #21836

    Depositor

    David Ron

    Insert

    EGFP
    

    Use

    Retroviral

    Tags

    farnysylation signal

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3 Connexin43-GFP-APEX2

    Plasmid

    #49385

    Purpose

    Expresses a tandem GFP-APEX2 fusion to the C-terminus of connexin43 in mammalian cells

    Depositor

    Alice Ting

    Article

    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

    Insert

    Connexin43 - EGFP - APEX2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K14D, W41F, E112K, A134P on soybean APX; improved…

    Promoter

    CMV

    Available since

    Nov. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX601 miniCMV-SaCas9-SpA-sgRNA scaffold

    Plasmid

    #107055

    Purpose

    Backbone vector for cloning in target sgRNA for use with SaCas9 (SauCas9)

    Depositor

    Alex Hewitt

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. mito-PAGFP

    Plasmid

    #23348

    Purpose

    Expresses a mitochondrial matrix-targeted photoactivable GFP that enables detection and quantification of organelle fusion in living cells.

    Depositor

    Richard Youle

    Article

    Karbowski et al J Cell Biol. 2004 Feb 16. 164(4):493-9.

    Insert

    PAGFP
    

    Use

    Tags

    mito

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. Neurl1-Flag pcDNA3.1

    Plasmid

    #17282

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

    Insert

    Neuralized-like (Neurl1a Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2008

    Availability

    Academic Institutions and Nonprofits only