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165,872 results

Showing: 6381 - 6400 of 165872 results

  1. C-Terminal Split Cas9 with GyrA intein

    Plasmid
    #58694
    Purpose
    Expresses truncated C-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with N-Terminal Split Cas9 Gyra Intein for full length SpCas9 production
    Depositor
    Gang Bao
    Article
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    Humanized C-Terminal S. pyogenes Cas9 with GyrA Csplit Intein
    Use
    AAV and CRISPR
    Tags
    GyrA Csplit Intein and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available Since
    July 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    58694-plasmid-map-sequence-id-90691

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  2. pCAG-eCas9-GFP-U6-gRNA

    Plasmid
    #79145
    Purpose
    All-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9 expression in human pluripotent stem cells
    Depositor
    Jizhong Zou
    Insert
    eSpCas9(1.1)
    Use
    CRISPR
    Tags
    2A-GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available Since
    June 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    79145-plasmid-map-sequence-id-182555

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  8. pENN.AAV.cTNT.PI.eGFP.WPRE.rBG (AAV9)

    Viral Prep
    #105543-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pENN.AAV.cTNT.PI.eGFP.WPRE.rBG (#105543). In addition to the viral particles, you will also receive purified pENN.AAV.cTNT.PI.eGFP.WPRE.rBG plasmid DNA. cTNT-driven eGFP. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    cTNT
    Tags
    EGFP
    Available Since
    July 24, 2018
    Availability
    Academic Institutions and Nonprofits only

  9. pKI_ΩBBMVP16&SRDXWUSm1

    Plasmid
    #220349
    Purpose
    Expresses BBM-VP16 and SRDX-WUSm1 in plant cells
    Depositor
    Tomoko Igawa
    Article
    Sato et al Front Plant Sci. 2024 Apr 3;15:1308417.
    Inserts
    BABY BOOM (BBM Mustard Weed)
    WUSCHEL (WUS Mustard Weed)
    Use
    Tags
    Superman Repression Domain X (SRDX) and VP16 tran…
    Expression
    Plant
    Mutation
    two amino acid substitution in WUS-box: L255A L25…
    Promoter
    CaMV 35S and RPS5A
    Available Since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    220349-plasmid-map-sequence-id-440868

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  13. RHO3

    Bacterial Strain
    #124700
    Purpose
    E. coli mobilizer strain that facilitates conjugation of mobilizable plasmids by using metabolic counter-selection against the donor strain.
    Depositor
    Herbert Schweizer
    Article
    Lopez et al Appl Environ Microbiol. 2009 Oct;75(20):
    Bacterial Resistance
    Dap (400 µg/ml)
    Available Since
    April 5, 2021
    Availability
    Academic Institutions and Nonprofits only

  15. 3XMEF2-luc

    Plasmid
    #32967
    Purpose
    Reporter gene for MEF2 activity with 3 MEF2 sites upstream of a c-fos minimal promoter
    Depositor
    Ron Prywes
    Insert
    MEF2 binding sites (MEF2A Human)
    Use
    Luciferase
    Tags
    Luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    MEF2
    Available Since
    Feb. 2, 2012
    Availability
    Academic Institutions and Nonprofits only
    32967-plasmid-map-sequence-id-228104

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  16. pCMV-T7-ABE8e-nSpCas9-P2A-EGFP (KAC978)

    Plasmid
    #185910
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    human codon optimized ABE8e-nSpCas9-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A
    Promoter
    CMV and T7
    Available Since
    June 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    185910-plasmid-map-sequence-id-363739

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Showing: 6381 - 6400 of 165872 results