188,072 results
-
Plasmid#182310PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into astrocytes via NFIA and SOX9 expressionUsePiggybacTagsNone and T2A-mycNLS-mTagBFP2ExpressionMammalianPromoterEF1a and TRE3GAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only
-
Nsp3 -EGFP
Plasmid#165108Purposemammalian expression and localizationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNsp3 (ORF1ab SARS-COV-2)
TagsEGFPExpressionMammalianPromoterCMVAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCh-Mfn2
Plasmid#141156PurposeMammalian expression and labeling mitofusin 2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMitofusin 2 (MFN2 Human)
TagsmCherryExpressionMammalianPromoterCMVAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
8xNFAT-ZsG-hCD8
Plasmid#153417PurposeNFAT-driven ZsGreen-1 reporter gene with human CD8ab geneDepositorInsert8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (Cd8a )
UseRetroviralExpressionMammalianAvailable sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTP264
Plasmid#149334PurposeBacterial expression of Biotin ligase BirADepositorInsertBiotin ligase BirA
TagsHis14 and bdNEDD8ExpressionBacterialAvailable sinceJune 18, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-Ef1a-fDIO mCherry (AAV1)
Viral prep#114471-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-Ef1a-fDIO mCherry (#114471). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO mCherry plasmid DNA. Ef1a-driven, Flp recombinase-dependent expression of mCherry. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsmCherry (Flp-dependent)Available sinceDec. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hChR2(H134R)-mCherry (AAV Retrograde)
Viral prep#26975-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-CaMKIIa-hChR2(H134R)-mCherry (#26975). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hChR2(H134R)-mCherry plasmid DNA. CamKIIa-driven, humanized channelrhodopsin H134R mutant fused to mCherry for optogenetic activation. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIITagsmCherryAvailable sinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 TFAM-mScarlet
Plasmid#129573PurposeExpression of mScarlet fused TFAMDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTFAM (TFAM Human)
ExpressionMammalianMutationNoneAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-HA-NONO
Plasmid#127655PurposeOver-express HA-tagged human NONODepositorAvailable sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMGS46 (ARF16-PB1-HA-P2A-OsTIR1)
Plasmid#126580PurposeA repair construct to express ARF16-PB1-HA and OsTIR1 under the control of the CMV promoter from the human AAVS1 locusDepositorInsertOsARF16-PB1 domain
TagsHAExpressionMammalianAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR-pSFFV-dCas9-SunTag-P2A-BFP-dWPRE
Plasmid#122151PurposeExpresses dCas9-SunTag(24X) fusion and a fluorescent indicator BFP.DepositorInsertdCas9 fused to SunTag(24x)
UseLentiviralTags2xNLS-P2A-BFP and NLSExpressionMammalianPromoterSFFVAvailable sinceMarch 6, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHAGE-BRAF-V600E
Plasmid#116204PurposeLentiviral expression of BRAF V600EDepositorAvailable sinceJan. 11, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.Syn.Flex.GCaMP6m.WPRE.SV40 (AAV1)
Viral prep#100838-AAV1PurposeReady-to-use AAV1 particles produced from pAAV.Syn.Flex.GCaMP6m.WPRE.SV40 (#100838). In addition to the viral particles, you will also receive purified pAAV.Syn.Flex.GCaMP6m.WPRE.SV40 plasmid DNA. Syn-driven, Cre-dependent GCaMP6M calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn.Flex.GCaMP6m.WPRE.SV40 (AAV9)
Viral prep#100838-AAV9PurposeReady-to-use AAV9 particles produced from pAAV.Syn.Flex.GCaMP6m.WPRE.SV40 (#100838). In addition to the viral particles, you will also receive purified pAAV.Syn.Flex.GCaMP6m.WPRE.SV40 plasmid DNA. Syn-driven, Cre-dependent GCaMP6m calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-U6insert-EF1puro
Plasmid#104537PurposeA piggybac vector with puromycin resistance and a U6 promoter that allows cloning of guide RNAsDepositorTypeEmpty backboneUseCRISPR, Mouse Targeting, and Synthetic Biology ; …ExpressionMammalianPromoterU6Available sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-Ef1a-DIO EYFP (AAV2)
Viral prep#27056-AAV2PurposeReady-to-use AAV2 particles produced from pAAV-Ef1a-DIO EYFP (#27056). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO EYFP plasmid DNA. EF1a-driven, cre-dependent EYFP expression. These AAV preparations are suitable purity for injection into animals.DepositorTagsEYFP (Cre-dependent)Available sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
LbCpf1-2NLS
Plasmid#102566Purposebacterial expression of LbCpf1-2NLSDepositorInsertLbCpf1
Tags6xHis-MBP-TEV and HA-2xSV40NLSExpressionBacterialPromoterT7Available sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX330-EN1201
Plasmid#92144PurposeFor transient expression of spCas9-nuclease and a sgRNA targeting the mouse TIGRE acceptor locusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertspCas9-nuclease and sgRNA against mouse TIGRE acceptor locus
ExpressionMammalianAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
6-His-MBP-TEV-FnCpf1
Plasmid#90094PurposeBacterial expression plasmid for protein purificationDepositorInsertFnCpf1 (humanized)
Tags3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…ExpressionBacterialPromoterT7Available sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Human CRISPR lncRNA activation library (SAM) in lenti sgRNA(MS2)_zeo backbone
Pooled library#1000000106PurposeHuman CRISPR Synergistic Activation Mediator (SAM) pooled library for the transcriptional activation of lncRNAsDepositorAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only