168,321 results

1. pAAV-hsyn-GRAB_DA2m

   Plasmid

   #140553

   Purpose

   Expresses the 2nd generation medium-affinity DA sensor GRAB_DA2m in neurons

   Depositor

   Yulong Li

   Article

   Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

   Has Service

   AAV9

   Insert

   The 2nd generation DA sensor GRAB_DA2m (medium affinity)
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   July 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPAGFP-N1

   Plasmid

   #11909

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Patterson et al Science. 2002 Sep 13. 297(5588):1873-7.

   Has Service

   Cloning Grade DNA

   Insert

   pPAGFP-N1
   

   Expression

   Mammalian

   Mutation

   These plasmids are essentially the same as the Cl…

   Available Since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. SpyTag003-sfGFP

   Plasmid

   #133454

   Purpose

   Expresses SpyTag003-superfolder GFP in bacterial cytoplasm

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   SpyTag003-sfGFP
   

   Tags

   GSGSGS linker and His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. Sybody Generation Toolbox

   Plasmid Kit

   #1000000160

   Purpose

   The Seeger Lab protocol to select synthetic nanobodies – called sybodies – against (membrane) protein targets. This collection contains all plasmids necessary for the sybody generation.

   Depositor

   Markus Seeger

   Available Since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

5. 3xFlag-tdMCP-Erk2 (pErk reporter)

   Plasmid

   #226167

   Purpose

   Used to transiently express the pErk reporter in LABEL-seq MAPK activity assays.

   Depositor

   Dustin Maly

   Article

   Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

   Insert

   3xFlag-tdMCP-Erk2 (MAPK1 Human)
   

   Tags

   3xFlag-tdMCP

   Expression

   Mammalian

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1_AGIA-AirID

   Plasmid

   #182210

   Purpose

   Express AirID in mammalian cells.

   Depositor

   Sohei Ito, Shogo Nakano, Akira Nozawa, Tatsuya Sawasaki

   Article

   Kido et al Elife. 2020 May 11;9. pii: 54983. doi: 1

   Insert

   AGIA-AirID
   

   Tags

   AGIA-tag

   Expression

   Mammalian

   Available Since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-ApoE3

   Plasmid

   #197147

   Purpose

   Expresses ApoE3 in Mammalian cell

   Depositor

   Bradley Hyman

   Article

   Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

   Insert

   ApoE3 (APOE Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTEI039

   Plasmid

   #105428

   Purpose

   Synthetic biology

   Depositor

   Johannes Stuttmann

   Article

   Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

   Insert

   LexA-VP16-ER (transcription factor: inducible expression; estradiol)
   

   Use

   Synthetic Biology

   Mutation

   BsaI/ BpiI restriction sites removed

   Available Since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. CMV500 A-CREB

   Plasmid

   #33371

   Depositor

   Charles Vinson

   Article

   Ahn et al Mol Cell Biol. 1998 Feb . 18(2):967-77.

   Insert

   CREB (Creb1 Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Dominant Negative (see notes)

   Promoter

   CMV

   Available Since

   Dec. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRK5-myc-Miro1

    Plasmid

    #47888

    Purpose

    Expresses myc tagged Miro1

    Depositor

    Pontus Aspenström

    Article

    Fransson et al J Biol Chem. 2003 Feb 21;278(8):6495-502

    Insert

    Miro1 (RHOT1 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. HIS-HA-CMYC P53

    Plasmid

    #233738

    Purpose

    expresses HIS-HA-CMYC triple tagged P53 for pulldown assay

    Depositor

    Albert Keung

    Article

    Han et al Methods. 2025 Mar;235:92-99. doi: 10.101

    Insert

    HIS-HA-CMYC-p53 (TP53 Human)
    

    Tags

    HIS-HA-CMYC

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV Retrograde)

    Viral Prep

    #112677-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Promoter

    EF1a

    Tags

    mCherry (Cre-negative cells), EGFP (Cre-positive cells)

    Available Since

    July 16, 2019

    Availability

    Academic Institutions and Nonprofits only

13. pPGK-SB13

    Plasmid

    #236078

    Purpose

    SB13 transposase, for use with Sleeping Beauty transponson plasmids

    Depositor

    Masashi Narita

    Article

    Chan et al Nature. 2024 Sep;633(8030):678-685. doi:

    Insert

    SB13 transposase
    

    Use

    Sleeping beauty (sb) transposase plasmid

    Promoter

    PGK

    Available Since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBPnlsLexA::p65Uw

    Plasmid

    #26230

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

    Insert

    nlsLexA::p65 (RELA Human)
    

    Expression

    Insect

    Available Since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCWU6-xylR-E-luciferase

    Plasmid

    #190721

    Purpose

    fine tune expression of luciferase under the control of xylose- and theophylline- inducible system

    Depositor

    Chenggang Wu

    Article

    Bibek et al bioRxiv. 2023 Apr 24:2023.04.24.538132.

    Insert

    xylR-E-luciferase
    

    Use

    Luciferase

    Expression

    Bacterial

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. hAAVS1 1R TALEN

    Plasmid

    #35432

    Purpose

    TALEN designed to target the human AAVS1 locus

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

    Insert

    hAAVS1 1R TALEN
    

    Expression

    Mammalian

    Available Since

    April 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCW57.1-4EBP1_4xAla

    Plasmid

    #38240

    Depositor

    David Sabatini

    Article

    Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

    Insert

    4EBP1 (Eif4ebp1 hybrid gene of rat and human, Rat)
    

    Use

    Lentiviral; Doxycycline inducible

    Expression

    Mammalian

    Mutation

    T37A, T46A, S65A, T70A (dominant negative)

    Promoter

    Tet ON

    Available Since

    Nov. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHIV-EF1a-2A-Ovalbumin(dN)-ZsGreen

    Plasmid

    #231986

    Purpose

    Empty backbone for expression of Ovalbumin with Bi-cistronic expression ZsGreen

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    OVA-2A-ZsGreen
    

    Use

    Lentiviral

    Available Since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. mTurquoise2

    Plasmid

    #198196

    Purpose

    Spectral correction: Expression of mTurquoise2

    Depositor

    Christian Wahl-Schott

    Article

    Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

    Insert

    mTurquoise2
    

    Expression

    Mammalian

    Mutation

    A206K

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMT870 human L1 RT- (ORF2p D702Y) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid

    #213027

    Purpose

    Expresses reverse transcriptase dead (RT- ORF2p D702Y) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 (ORFeus-Hs codon optimized sequence, RT- D702Y) with ORF2-3xFlag
    

    Tags

    3xFlag (ORF2p)

    Expression

    Mammalian

    Mutation

    D702Y (RT- reverse transcriptase catalytic dead)

    Promoter

    CMV

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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