165,888 results

1. pcDNA3.1(-)-qmTQ2-ATP

   Plasmid

   #225248

   Purpose

   mammalian expression of qmTQ2-ATP, a cyan fluorescent FLIM biosensor for ATP imaging

   Depositor

   Yasushi Okada

   Article

   Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

   Insert

   qmTQ2-ATP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available Since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.3_d2eGFP

   Plasmid

   #26821

   Depositor

   Derrick Rossi

   Article

   Warren et al Cell Stem Cell. 2010 Nov 5;7(5):618-30.

   Insert

   5'UTR-d2eGFP-3'UTR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   destabilized (PEST sequence) GFP

   Promoter

   Available Since

   Nov. 17, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pmCherry-2xFYVE

   Plasmid

   #140050

   Purpose

   Expresses mCherry-tagged HRS 2xFYVE in mammalian cells

   Depositor

   Harald Stenmark

   Article

   Sneeggen et al Nat Commun. 2019 Jun 28;10(1):2850. doi:

   Insert

   tandem HRS FYVE domain (Hgs Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   two FYVE domains (amino acids 147-223), linked by…

   Promoter

   CMV

   Available Since

   May 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTW2082

   Plasmid

   #236576

   Purpose

   ISYmu1 PDS3 gRNA12 TRV2 Architecture_B

   Depositor

   Steven Jacobsen

   Article

   Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

   Insert

   ISYmu1 TnpB
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. PH-Akt-GFP

   Plasmid

   #51465

   Purpose

   Is a biosensor for PIP3/PI(3,4)P2

   Depositor

   Tamas Balla

   Article

   Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

   Insert

   AKT (aa 1–164)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3/Myc-DNMT3B1

   Plasmid

   #35522

   Purpose

   Mammalian expression of human DNMT3B1 with myc tag

   Depositor

   Arthur Riggs

   Article

   Chen et al J Cell Biochem. 2005 Aug 1;95(5):902-17.

   Insert

   DNMT3B1 (DNMT3B Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 9, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lenti-EF1alpha-dCas9-p300_Blast

   Plasmid

   #192653

   Purpose

   3rd generation lenti vector encoding dCas9-p300 with 2A Blast resistance marker

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   dCas9-p300
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A; H840A in Cas9

   Promoter

   EF1a

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTA-Mob 2.0

   Plasmid

   #149662

   Purpose

   Conjugation helper plasmid for use in cis (self-transfer) and trans (transfer of plasmids with RK2/RP4 origin of transfer) mobilization to prokaryotic and eukaryotic cells.

   Depositor

   Bogumil Karas

   Article

   Soltysiak et al Int J Mol Sci. 2019 Oct 21;20(20). pii:

   Type

   Empty backbone

   Use

   Synthetic Biology; Conjugation helper plasmid

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTRKH2

   Plasmid

   #71312

   Purpose

   Theta replicating shuttle vector for E. coli and gram positive bacteria. High copy number in gram positives. Erythromycin resistance, recommended to use BHI agar plates for E. coli.

   Depositor

   Todd Klaenhammer, Rodolphe Barrangou

   Article

   O'Sullivan et al Gene. 1993 Dec 31;137(2):227-31.

   Type

   Empty backbone

   Use

   E. coli-gram positive bacteria shuttle vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. PABPC1

    Plasmid

    #155805

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    PABPC1 (PABPC1 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. CLYBL-TO-hNGN2-BSD-mApple

    Plasmid

    #124229

    Purpose

    Targets the human CLYBL safe harbor locus for integration of dox-inducible NGN2 for differentiation of iPSCs into cortical neurons. Contains a floxed BSD-NLS-mApple selection cassette.

    Depositor

    Michael Ward

    Article

    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

    Insert

    NGN2 (NEUROG2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFP-hPIKfyve

    Plasmid

    #121148

    Purpose

    Expresses GFP-tagged human PIKfyve in mammalian cells.

    Depositor

    Geert van den Bogaart

    Article

    Baranov et al iScience. 2019 Jan 25;11:160-177. doi: 1

    Insert

    1-phosphatidylinositol 3-phosphate 5-kinase isoform 2 (PIKFYVE Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti6.3 GCN1-3xFlag_IRES-iRFP

    Plasmid

    #198388

    Purpose

    GCN1 expression

    Depositor

    Jack Taunton

    Article

    Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

    Insert

    GCN1 (GCN1 Human)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTetOne-human cGAS-HA

    Plasmid

    #186896

    Purpose

    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

    Insert

    Human cGAS with C-terminal HA tag (Adamts1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    TRE3G

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. gRNA_AAVS1-T2

    Plasmid

    #41818

    Purpose

    Expresses a guide RNA (gRNA) to target human AAVS1 (T2 target sequence) for genome engineering

    Depositor

    George Church

    Article

    Mali et al Science. 2013 Feb 15;339(6121):823-6. do

    Insert

    gRNA_AAVS1-T2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. Cyto-B

    Plasmid

    #209858

    Purpose

    Dimerization dependent fluorescent protein B fused to NES

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddGFP B
    

    Use

    Tags

    NES sequence LALKLAGLDIGS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA

    Plasmid

    #182653

    Purpose

    Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF),tRNACUAPyl & eRF1E55D used for amber codon suppression in mammalian cells

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Inserts

    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    mutant eukaryotic release factor 1
    

    Use

    Tags

    HA tag and nuclear export signal (NES)

    Expression

    Mammalian

    Mutation

    E55D and Y306A/Y384F (AF) double mutant of Methan…

    Promoter

    CMV and U6

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pVQ CMV NanoV1-2a-EGFP ferritin

    Plasmid

    #79649

    Purpose

    Expresses camelid anti-GFP nanobody fused to TRPV1 and GFP-ferritin chimera fusion protein

    Depositor

    Jeffrey Friedman

    Article

    Stanley et al Nature. 2016 Mar 31;531(7596):647-50. do

    Inserts

    transient receptor potential vanillin 1 (Trpv1 Rat)
    camelid anti-GFP nanobody
    Enhanced green fluorescent protein
    mouse ferritin light chain (Ftl1 Mouse)
    mouse ferritin heavy chain (Fth1 Mouse)
    

    Use

    Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEGFP-2xFYVE

    Plasmid

    #140047

    Purpose

    Expresses GFP-tagged HRS 2xFYVE in mammalian cells

    Depositor

    Harald Stenmark

    Article

    Gillooly et al EMBO J. 2000 Sep 1;19(17):4577-88. doi:

    Insert

    tandem HRS FYVE domain (Hgs Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    two FYVE domains (amino acids 147-223), linked by…

    Promoter

    CMV

    Available Since

    May 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HDAC4 Flag

    Plasmid

    #13821

    Purpose

    Mammalian expression of human histone deacetylase 4 with flag tag

    Depositor

    Eric Verdin

    Article

    Fischle et al J Biol Chem. 1999 Apr 23. 274(17):11713-

    Insert

    HDAC4 (HDAC4 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge