161,383 results

1. pCEP4-myc-ACE2

   Plasmid

   #141185

   Purpose

   Mammalian expression plasmid for human ACE2 with an N-terminal (extracellular) c-myc tag

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Use

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-EGFP-Cdc42(T17N)

   Plasmid

   #12601

   Depositor

   Klaus Hahn

   Article

   Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

   Insert

   EGFP-Cdc42 (T17N) (CDC42 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   T17N

   Promoter

   Available since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHL-sec

   Plasmid

   #99845

   Purpose

   Mammalian expression with secretion signal sequence

   Depositor

   Edith Yvonne Jones

   Article

   Aricescu et al Acta Crystallogr D Biol Crystallogr. 200

   Insert

   ephrin b2 (EFNB2 Human)
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-EFS-DIO-GRAB_OT1.0

   Plasmid

   #185387

   Purpose

   Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in a cre-dependent manner

   Depositor

   Yulong Li

   Article

   Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   GPCR activation based oxytocin sensor GRAB_OT1.0
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EFS

   Available since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJH376

   Plasmid

   #86842

   Purpose

   mamalian expression of anti-CRISPR protein AcrIIA4

   Depositor

   Joseph Bondy-Denomy

   Article

   Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

   Insert

   acrIIA4
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDH-EF1-Nluc-P2A-copGFP-T2A-Puro

   Plasmid

   #73022

   Purpose

   Luminescent and fluorescent dual reporter

   Depositor

   Kazuhiro Oka

   Insert

   Nluc-P2A-copGFP-T2A-Puro
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1

   Available since

   Jan. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. MG15-luc (mut p53 binding sites)

   Plasmid

   #16443

   Depositor

   Bert Vogelstein

   Article

   el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

   Insert

   p53-binding site (mutant) (TP53 )
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGGS-FuG-B2

   Plasmid

   #67513

   Purpose

   This is an envelope gene encoding plasmid to make retrogradely lentiviral vector. You can make type B2 envelope coating virus particle with HiRet by using this plasmid.

   Depositor

   Kazuto Kobayashi

   Article

   Kato et al J Neurosci. 2011 Nov 23;31(47):17169-79.

   Insert

   Fusion Glycoprotein type B2
   

   Use

   Lentiviral

   Tags

   Fusion protein of Vesicular stomatitis Indiana vi…

   Expression

   Mutation

   Promoter

   CAGGS

   Available since

   July 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set B

   Pooled Library

   #92380

   Purpose

   CRISPRa

   Depositor

   John Doench, David Root

   Article

   Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

   Use

   Lentiviral

   Available since

   Aug. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3 CB1

    Plasmid

    #13391

    Purpose

    Expresses mouse cannabinoid receptor 1 (CB1) in mammalian cells

    Depositor

    Mary Abood

    Article

    Abood et al Biochem Pharmacol. 1997 Jan 24. 53(2):20

    Insert

    CB1 (Cnr1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-TRE-eYFP

    Plasmid

    #104056

    Purpose

    An AAV genome with tet-inducible expression of the fluorescent protein eYFP

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Insert

    EYFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    TRE

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKAM-BFP-Puro

    Plasmid

    #101864

    Purpose

    Lentiviral empty backbone, dual BFP and puro markers

    Depositor

    Archibald Perkins

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFBDM

    Plasmid

    #110738

    Purpose

    Plasmid for the insect cell expression system, two promoters, Multiplication Module M, Tn7 transposition

    Depositor

    Tim Richmond

    Article

    Fitzgerald et al Nat Methods. 2006 Dec;3(12):1021-32. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin and p10 promoters

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMT010

    Plasmid

    #64762

    Purpose

    Recombination-Induced Tag Exchange (RITE) Cassette, HA to a 2xFLAG epitope tag, Hygro selection

    Depositor

    Fred van Leeuwen

    Article

    Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

    Insert

    RITE cassette
    

    Use

    Rite cassette

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. mEGFP-NbALFA

    Plasmid

    #159986

    Purpose

    For the expresson and purification of the EGFP-tagged anti-ALFA tag nanobody (NbALFA)

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Insert

    mEGFP-NbALFA
    

    Use

    Tags

    8histidine-HRV3C protease cleavage site

    Expression

    Bacterial

    Mutation

    3GS linker between mEGFP and NbALFA

    Promoter

    Available since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. B-gTEMP/pcDNA3

    Plasmid

    #188447

    Purpose

    B-gTEMP for mammalian expression

    Depositor

    Takeharu Nagai

    Article

    Lu et al Nano Lett. 2022 Jul 27;22(14):5698-5707.

    Insert

    B-gTEMP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJ3M-Mst2

    Plasmid

    #12205

    Depositor

    Jonathan Chernoff

    Article

    Creasy et al J Biol Chem. 1996 Aug 30. 271(35):21049-

    Insert

    Mst2 (STK3 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. FUGW-VMAT2-pHluorin

    Plasmid

    #207468

    Purpose

    Lentiviral expression of VMAT2-pHluorin

    Depositor

    Robert Edwards

    Article

    Silm et al Neuron. 2019 May 22;102(4):786-800.e5. d

    Insert

    VMAT2 (Slc18a2 Rat)
    

    Use

    Lentiviral

    Tags

    pHluorin

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAGGS-myrGFP

    Plasmid

    #115502

    Purpose

    expression of myristoylated, plasma membrane targeted GFP

    Depositor

    Thomas Biederer

    Article

    Stagi et al Proc Natl Acad Sci U S A. 2010 Apr 20;10

    Insert

    myristoylated GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    chicken beta-actin

    Available since

    Jan. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

20. pscALPSpuro-MmACE2 (Mouse)

    Plasmid

    #158087

    Purpose

    Expresses mouse ACE2 in mammalian cells

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    Mouse ACE2 (Ace2 Mouse)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge