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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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182,244 results

Showing: 6401 - 6420 of 182244 results

  1. R777-E283 Hs.ROCK1

    Plasmid
    #70567
    Purpose
    Gateway ORF clone of human ROCK1 [NM_005406.2] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    ROCK1 (ROCK1 Human)
    Use
    Gateway Cloning
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70567-plasmid-map-sequence-id-126016

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  3. MSP2135

    Plasmid
    #72248
    Purpose
    Human expression plasmid for SpCas9-HF2 variant: CMV-T7-humanSpCas9-HF2(N497A, R661A, Q695A, Q926A, D1135E)-NLS-3xFLAG
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 HF2(N497A/R661A/Q695A/Q926A/D1135E)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A, and D1135E
    Promoter
    CMV
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    72248-plasmid-map-sequence-id-131868

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  6. pMito-rxRFP1.1

    Plasmid
    #67841
    Purpose
    redox-sensitive red fluorescent protein rxRFP1.1, mitochondrially localized
    Depositor
    Huiwang Ai
    Publication
    Fan et al Anal Bioanal Chem. 2016 Jan 12.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    rxRFP1.1 and mitochondrial localization sequence
    Tags
    His Tag and mitochondrial localization sequence
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    67841-plasmid-map-sequence-id-244286

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  8. MSP680

    Plasmid
    #65772
    Purpose
    Human expression vector for SpCas9 EQR variant: CMV-T7-humanSpCas9(D1135E/R1335Q/T1337R)-NLS-3xFLAG (EQR variant)
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E/R1335Q/T1337R)-NLS-3XFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135E, R1335Q, and T1337R mutations in Cas9
    Promoter
    CMV & T7
    Available since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    65772-plasmid-map-sequence-id-112654

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  11. pVITRO1-Trastuzumab-IgG2/κ

    Plasmid
    #61884
    Purpose
    Expresses HER2/neu receptor specific humanized IgG2/κ antibody isotype (Trastuzumab-IgG2/κ)
    Depositor
    Andrew Beavil
    Publication
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    HER2/neu receptor specific humanized Gamma 2 heavy chain expression cassette
    HER2/neu receptor specific humanized kappa light chain expression cassette
    Expression
    Mammalian
    Promoter
    mEF1 Prom and rEF1 Prom
    Available since
    March 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    61884-plasmid-map-sequence-id-207869

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  12. GFP-Rab4B

    Plasmid
    #61801
    Purpose
    Human Rab4B fluorescently tagged with GFP on the N-terminus for mammalian expression
    Depositor
    Gia Voeltz
    Publication
    Rowland et al Cell. 2014 Nov 20;159(5):1027-41. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Rab4B (RAB4B Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available since
    Jan. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    61801-plasmid-map-sequence-id-454375

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  13. pHSN401

    Plasmid
    #50588
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Publication
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    3×FLAG-NLS-zCas9-NLS
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG and NLS
    Mutation
    maize codon optimized
    Promoter
    2×35Sp and AtU6-26p
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    50588-plasmid-map-sequence-id-75660

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  14. pAluYa5-neo-TET

    Plasmid
    #51283
    Purpose
    expresses Alu, a human SINE, using the pol III enhancer sequence of the 7SL gene and tagged with the self splicing intron neo cassette
    Depositor
    Astrid Roy-Engel
    Publication
    Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:
    Insert
    AluYa5
    Tags
    neoTET cassette with a tetrahymena self-splicing …
    Expression
    Mammalian
    Promoter
    upstream pol III enhancer sequence of the 7SL gen…
    Available since
    April 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    51283-plasmid-map-sequence-id-78362

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  15. GABA(A) receptor subunit a1SE

    Plasmid
    #49168
    Purpose
    pHluorin-tagged GABA A receptor subunit (alpha 1) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface
    Depositor
    Tija Jacob, Stephen Moss
    Publication
    Tretter et al J Neurosci. 2008 Feb 6;28(6):1356-65. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GABA(A) receptor subunit alpha-1 (Gabra1 Mouse)
    Tags
    myc and pHGFP (pHIuorin)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    49168-plasmid-map-sequence-id-72484

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  19. psd44-GqQL

    Plasmid
    #46826
    Purpose
    2nd generation lentiviral vector for expression of mutant G protein alpha q in mammalian cells
    Depositor
    Agnese Mariotti
    Insert
    g protein alpha q (GNAQ Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    Q209L (The mutation reduces GTPase activity resul…
    Promoter
    PGK promoter
    Available since
    July 30, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    46826-plasmid-map-sequence-id-246440

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Showing: 6401 - 6420 of 182244 results