173,139 results
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Plasmid#146574PurposeMammalian Expression of HsUPF3biso2DepositorInsertHsUPF3biso2 (UPF3B Human)
ExpressionMammalianMutationone silent mutation T510C compared to the sequenc…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-TRP1-pCUP1-2xphlO
Plasmid#191794PurposeYeast gene induction with copper promoterDepositorInsertTRP1 pCUP1 with 2 phlO sites
UseYeast genomic targetingAvailable SinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTOPO-col10a1-KI-donor
Plasmid#184874PurposeDonor template for knock-in of p2a-CreERT2 into the medaka col10a1 locus via CRISPR/Cas9-mediated homology directed repairDepositorInsertsCollagen10a1 5' homology arm
Collagen10a1 3' homology arm
p2a-CreERT2
Myosin light chain 2 promoter
eGFP
UseCRISPR and Cre/LoxAvailable SinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG_ToeRep_N09_trigger
Plasmid#132740PurposeT7 RNAP-driven expression of first-generation toehold repressor index 9 trigger RNADepositorInsertToeRep_N09_trigger
ExpressionBacterialAvailable SinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYZ_Ptet*_3WJrep_N19_switch
Plasmid#132739PurposeInducible expression of 3WJ repressor index 19 switch RNA with a GFP-ASV reporter via the Ptet* promoterDepositorInsertPtet*_3WJrep_N19_switch
ExpressionBacterialAvailable SinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDH-puro/Flag PD-L1 N219Q
Plasmid#121452Purposeexpression of huamn PD-L1 mutant in mammalian cellsDepositorInsertCD274 (CD274 Human)
UseLentiviralTagsFlagExpressionMammalianMutationN219QPromoterCMVAvailable SinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-Neo
Plasmid#25735Purpose3rd generation Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Neomycin resistance gene coexpression.DepositorTypeEmpty backboneUseLentiviral and RNAiExpressionMammalianAvailable SinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSL1119 (pDonor)
Plasmid#160732PurposeMini-transposon donor plasmid for V. cholerae CAST system. pUC19 vector backbone.DepositorInsertVchCAST donor DNA (Mini-Tn)
ExpressionBacterialPromoterN/AAvailable SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GfaABC1D-GRAB_ATP1.0
Plasmid#167579PurposeExpress the ATP sensor GRAB_ATP1.0 in astrocytesDepositorAvailable SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE28
Plasmid#90370PurposeTFEB - Lysosomal biogenesis gene reporterDepositorInsertFirefly luciferase
UseLentiviralPromoterTFEBAvailable SinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-mNeonGreen
Plasmid#99134PurposeAn AAV genome that ubiquitously expresses the fluorescent protein mNeonGreen from the CAG promoterDepositorInsertmNeonGreen
UseAAVExpressionMammalianPromoterCAGAvailable SinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-Chronos-GFP (AAV1)
Viral Prep#59170-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-Syn-Chronos-GFP (#59170). In addition to the viral particles, you will also receive purified pAAV-Syn-Chronos-GFP plasmid DNA. Syn-driven Chronos-GFP expression for optogenetic neural activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsGFPAvailable SinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDH-puro/Flag PD-L1 N200Q
Plasmid#121451Purposeexpression of human PD-L1 mutant in mammalian cellsDepositorInsertCD274 (CD274 Human)
UseLentiviralTagsFlagExpressionMammalianMutationN200QPromoterCMVAvailable SinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato (AAV8)
Viral Prep#60658-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato (#60658). In addition to the viral particles, you will also receive purified pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato plasmid DNA. CAG-driven, Flp-dependent expression of the voltage-gated Na+ channel mNaChBac-T2A-tdTomato. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1αAvailable SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHD-DsRed
Plasmid#51434PurposeVector for generating dsDNA donors for homology-directed repair. Contains the visible marker 3xP3-DsRed.DepositorInsertLoxP-3xP3-DsRed-LoxP
Promoter3xP3Available SinceFeb. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pgRNA-humanized
Plasmid#44248PurposeExpression of customizable guide RNA (gRNA) under cotnrol of murine U6 promoter, also contains a CMV-puro-t2A-mCherry expression cassette for mammalian gene knockdownDepositorInsertguide RNA
UseCRISPR and LentiviralExpressionMammalianPromotermouse U6Available SinceApril 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHREAC
Plasmid#134908PurposeOverexpression of recombinant proteins in plants thanks to very efficient translational efficiency. Empty vector, clone ORF using Bsa1 sites. Download fully annotated map under Resource Information.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionPlantPromoterdouble 35SAvailable SinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
Human Lentiviral sgRNA Library - Kinases
Pooled Library#51044PurposeSub-pool of Sabatini/Lander sgRNA library enriched for kinase targets.DepositorExpressionMammalianSpeciesHomo sapiensUseCRISPR and LentiviralAvailable SinceFeb. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG vGlut1 mCherry
Plasmid#105010PurposeExcitatory presynaptic markerDepositorInsertSLC17A7
ExpressionMammalianPromoterCAGAvailable SinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-SaCas9-U6-sgSlc17a6
Plasmid#124847PurposeMutagenesis of Slc17a6 with SauCas9DepositorInsertSlc17a6 gRNA (Slc17a6 Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable SinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only