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182,244 results

Showing: 6281 - 6300 of 182244 results

  2. pAAV-S5E2-GCaMP6f (AAV1)

    Viral prep
    #135632-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-S5E2-GCaMP6f (#135632). In addition to the viral particles, you will also receive purified pAAV-S5E2-GCaMP6f plasmid DNA. Expression of GCaMP6f under the control of the E2 regulatory element. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Jordane Dimidschstein
    Publication
    Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.
    Available since
    Nov. 11, 2020
    Availability
    Academic Institutions and Nonprofits only

  4. pET_RTX-6xHis

    Plasmid
    #145029
    Purpose
    pET vector for IPTG-inducible, T7-based expression of RTX bearing a C-terminal 6xHis tag
    Depositor
    Andrew Ellington
    Publication
    Bhadra et al BioProtocol 2021
    Insert
    RTX
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    T7
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    145029-plasmid-map-sequence-id-292639

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  6. pAAV-hSyn Con/Fon hChR2(H134R)-EYFP (AAV5)

    Viral prep
    #55645-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-hSyn Con/Fon hChR2(H134R)-EYFP (#55645). In addition to the viral particles, you will also receive purified pAAV-hSyn Con/Fon hChR2(H134R)-EYFP plasmid DNA. Synapsin-driven, Cre-dependent and Flp-dependent channelrhodopsin-EYFP expression for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Publication
    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:
    Promoter
    Syn
    Tags
    EYFP (Cre- and Flp-dependent)
    Available since
    Dec. 2, 2019
    Availability
    Academic Institutions and Nonprofits only

  10. pB-CAGGS-dCas9-KRAB

    Plasmid
    #110822
    Purpose
    PiggyBac compatible plasmid expressing dCas9-KRAB
    Depositor
    Alejandro Chavez, George Church
    Publication
    Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-KRAB
    Expression
    Mammalian
    Mutation
    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…
    Promoter
    CAGGS
    Available since
    July 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    110822-plasmid-map-sequence-id-265907

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  15. pWS158

    Plasmid
    #90517
    Purpose
    Cas9 gap repair vector - URA3
    Depositor
    Tom Ellis
    Insert
    Cas9
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Oct. 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    90517-plasmid-map-sequence-id-287206

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  17. pSFV-Helper1

    Plasmid
    #92073
    Purpose
    This is a plasmid for transcription of defective helper RNA, providing structural Semliki forest virus (SFV) proteins to package recombinant SFV genomes into virus like particles.
    Depositor
    Henrik Garoff
    Publication
    Liljestrom et al Biotechnology (N Y). 1991 Dec;9(12):1356
    Insert
    cDNA of SFV genome with deletion removing the nonstructural SFV proteins and also the viral RNA packaging signal.
    Use
    Helper plasmid, for run-off transcription and rna…
    Available since
    July 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92073-plasmid-map-sequence-id-275942

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  18. pY30

    Plasmid
    #84745
    Purpose
    Expresses huAsCpf1-P2A-puro and crRNA guide
    Depositor
    Feng Zhang
    Publication
    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    huAsCpf1
    puromycin resistance gene
    Tags
    3xHA, NLS, and T2A
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    84745-plasmid-map-sequence-id-162432

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Showing: 6281 - 6300 of 182244 results