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Plasmid#48353PurposeContributes the coding sequence for ECFP as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.DepositorInsertECFP
UseGateway entry vectorTagsExpressionMutationContains a stop codon.PromoternoneAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
amilGFP chromoprotein promoter-less
Plasmid#118006PurposeFor course in "Synthetic Biology: A Lab Manual"DepositorInsertRBS, amilGFP
UseSynthetic Biology; Escherichia coliTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYPQ292 (AaCas12b)-D570A-TV-MS2-VPR
Plasmid#136382PurposeCRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-VPR fusion proteinDepositorInsertAaCas12b (D570A)-TV-T2A-MCP-VPR
UseCRISPRTagsExpressionPlantMutationD570APromoterAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUT1159-HQ858703
Plasmid#27749DepositorInsertAUX/IAA transcription factor from DV030103
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceMarch 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pUT1455-HQ858749
Plasmid#27831DepositorInsertORPHAN transcription factor from DR801947
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceMarch 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pUT1682-HQ858766
Plasmid#27848DepositorInsertGRAS transcription factor from DV163953
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceMarch 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
p221z-erVenusYFP
Plasmid#71265PurposeEntry clone containing ER-localized Venus. Can be used to construct transcriptional reporters. For use in plants and compatible with the MultiSite Gateway systemDepositorInsertVenusYFP
UseGatewayTagsER retention signal HDEL and signal peptide (ER)…ExpressionMutationPromoterAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
tsPurple chromoprotein promoter-less
Plasmid#118007PurposeFor course in "Synthetic Biology: A Lab Manual"DepositorInsertRBS, tsPurple
UseSynthetic Biology; Escherichia coliTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXD71Cas10RFP-Pct5.1-crRNA(AarI)-RFP
Plasmid#192273PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(AarI)-RFP, entry plasmid for crRNA cloningDepositorInsertCymR
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Htr5b(3.7)-EGFP-sv40
Plasmid#184411PurposeAAV expression of EGFP from mouse 5-hydroxytryptamine (serotonin) receptor 5B (Htr5b) gene promoter.DepositorInsertMouse Htr5b gene promoter-EGFP (Htr5b Mouse)
UseAAVTagsEGFPExpressionMammalianMutationPromoterMouse Htr5b geneAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTarget TMEM97-P2A-mCardinal
Plasmid#157747PurposeExpress in mammalian cells TMEM97 and mCardianl to follow transfection efficiency using flow cytometry. mCardinal is expressed following a P2A skip peptide so it is not connected to TMEM97.DepositorInsertTMEM97 (TMEM97 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
p221z-erTagRFP
Plasmid#71266PurposeEntry clone containing ER-localized TagRFP. Can be used to construct transcriptional reporters. For use in plants and compatible with the MultiSite Gateway systemDepositorInsertTagRFP
UseGatewayTagsER retention signal HDEL and signal peptide (ER)…ExpressionMutationPromoterAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR P2R-P3-mKate2
Plasmid#48345PurposeContributes the coding sequence for the mKate2 fluorescent protein as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.DepositorInsertmKate2
UseGateway entry vectorTagsExpressionMutationContains a stop codon at the 3' end of the c…PromoternoneAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
p221k-erTq2CFP
Plasmid#71264PurposeEntry clone containing ER-localized Turqoise2. Can be used to construct transcriptional reporters. For use in plants and compatible with the MultiSite Gateway system.DepositorInsertTq2CFP
UseGatewayTagsER retention signal HDEL and signal peptide (ER)…ExpressionMutationPromoterAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFSynW Substance P-pHluorin IRES mRuby3
Plasmid#195699PurposeLentiviral plasmid encoding first 68 residues of mouse preprotachykinin with a C-terminal pHluorin tag followed by an internal ribosomal entry site followed by mRuby3 under the human synapsin promoterDepositorInsertTac1 (Tac1 Mouse)
UseLentiviralTagspHluorinExpressionMutationPromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-BNT162b2ΔUTR
Plasmid#171214PurposeGateway entry vector containing the BNT162b2/tozinameran cDNA sequence lacking the original 5' and 3' UTRs and the poly(A) tail. The original Kozak sequence has also been altered.DepositorInsertBNT162b2ΔUTR
UseGateway cloningTagsExpressionMutationKozak sequence changed from CCCGCCACC to GGCTGCAC…PromoterAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A hu_ncOGT WT (open N-term)
Plasmid#155197PurposeEntry vector encoding non-tagged O-GlcNAc Transferase with open N-terminusDepositorInsertO-GlcNAc Transferase (OGT Human)
UseGateway cloningTagsExpressionMutationWT OGT with no start codon, in-frame with gateway…PromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pME-CreLite
Plasmid#131782PurposeMiddle entry vector (Tol2 kit) with bi-cistronic CreLite system components, PhyBΔCreC and PIF6CreN, linked by P2A.DepositorInsertPhyBCreC-P2A-PIF6CreN
UseCre/Lox; Gateway cloningTagsExpressionMutationPromoterAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-MBD1
Plasmid#47057PurposeExpresses methyl-CpG binding domain protein 1DepositorInsertMethyl-CpG Binding Domain Protein 1 (MBD1 Human)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-5xUAS-sfGFP-GMRWhite
Plasmid#165907PurposeBlasticidin resistant 5xUAS sfGFP GMR-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screeningDepositorInsertSelectable UAS Response Vector
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only