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pET19b-rShHTL7 cPGFP(D165)

Plasmid

#166003

Purpose

Expression of coding sequence in Escherichia coli

Depositor

Claudia Vickers

Article

Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

Insert

rShHTL7 cPGFP(D165)

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pET19b-rShHTL7 cPGFP(C164)

Plasmid

#166002

Purpose

Expression of coding sequence in Escherichia coli

Depositor

Claudia Vickers

Article

Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

Insert

rShHTL7 cPGFP(C164)

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pET19b-DAD2 cPGFP(N180)

Plasmid

#166000

Purpose

Expression of coding sequence in Escherichia coli

Depositor

Claudia Vickers

Article

Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

Insert

DAD2 cPGFP(N180)

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pET19b-DAD2 cPGFP(A165)

Plasmid

#165999

Purpose

Expression of coding sequence in Escherichia coli

Depositor

Claudia Vickers

Article

Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

Insert

DAD2 cPGFP(A165)

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pET19b-HisDAD2 cPGFP(G164)

Plasmid

#165997

Purpose

Expression of coding sequence in Escherichia coli

Depositor

Claudia Vickers

Article

Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

Insert

HisDAD2 cPGFP(G164)

Tags

6His

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pET19b-rShHTL7 cPGFP(L166)

Plasmid

#165995

Purpose

Expression of coding sequence in Escherichia coli

Depositor

Claudia Vickers

Article

Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

Insert

rShHTL7 cPGFP(L166)

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pET19b-DAD2 cPGFP(G164)

Plasmid

#165993

Purpose

Expression of coding sequence in Escherichia coli

Depositor

Claudia Vickers

Article

Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

Insert

DAD2 cPGFP(G164)

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pAS1_4xh7SKPylT_EF1_BsmBI_(C1A)-Intein_DD

Plasmid

#162811

Purpose

Control STELLA destination plasmid for C-terminal labelled proteins. Contains restriction sites for golden gate cloning.

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

BsmBI_(C1A)-Intein_DD

Use

Piggybac

Expression

Mammalian

Mutation

Intein(Cys1,Ala)

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4xh7SKPylT_EF1_SMIM38*_Intein_DD

Plasmid

#162814

Purpose

STELLA plasmid for amber suppression of C-terminally labelled SMIM38

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

SMIM38(C-TAG)-Intein-DD

Use

Piggybac

Expression

Mammalian

Mutation

SMIM38(Ochre52,Amber)

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-BsmBI-HA_IRES_BSD

Plasmid

#162805

Purpose

STELLA destination plasmid with HA terminal tag; Suitable for amber suppression (4xMmaPylT cassette); for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-BsmBI-HA_IRES_Blasticidin

Use

Piggybac

Tags

HA

Expression

Mammalian

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pNP159

Plasmid

#106361

Purpose

To insert a single copy of a construct at a site on Chr II, corresponding to GFP-labelled RAB-11 expressed specifically in the epidermis.

Depositor

Jonathan Ewbank

Article

Taffoni et al Elife. 2020 Jan 29;9. pii: 45047. doi: 1

Insert

ttTi5605_SEC_dpy-7p_GFP_RAB-11_3'unc-54

Expression

Worm

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pMDC84-ScSEIPIN

Plasmid

#96984

Purpose

Express yeast SEIPIN gene in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

Insert

ScSEIPIN (SEI1 Budding Yeast)

Tags

6xHis and GFP

Expression

Plant

Promoter

35S

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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LI D-E 11GFP

Plasmid

#54430

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

FLAG tag + and short linker + superfolder GFP 11 domain

Use

Cloning vector

Available Since

Nov. 10, 2014

Availability

Academic Institutions and Nonprofits only

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SpDJ-1 pET15b

Plasmid

#60689

Purpose

Recombinant protein expression of DJ-1

Depositor

Mark Wilson

Article

Madzelan et al FEBS J. 2012 Nov;279(22):4111-20. doi: 1

Insert

DJ-1 (SPAC22E12.03c Fission Yeast)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only
pET28-ceRab2(1-214)

Plasmid

#59550

Purpose

makes 6x Fusion of Full length ceRab2

Depositor

David Lambright

Article

Chotard et al Mol Biol Cell. 2010 Jul 1;21(13):2285-96

Insert

1-214

Available Since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only
pET28-ceRab5(1-208)

Plasmid

#59551

Purpose

makes 6x Fusion of Full length ceRab5

Depositor

David Lambright

Article

Chotard et al Mol Biol Cell. 2010 Jul 1;21(13):2285-96

Insert

1-208

Tags

6x His

Available Since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only
pET28-ceRab10(1-201)

Plasmid

#59552

Purpose

makes 6x Fusion of Full length ceRab10

Depositor

David Lambright

Article

Chotard et al Mol Biol Cell. 2010 Jul 1;21(13):2285-96

Insert

1-208

Tags

6x His

Available Since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only
pET28-ceRab11(1-211)

Plasmid

#59553

Purpose

makes 6x Fusion of Full length ceRab11

Depositor

David Lambright

Article

Chotard et al Mol Biol Cell. 2010 Jul 1;21(13):2285-96

Insert

1-211

Tags

6x His

Available Since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only
pET28-ceRab21(1-207)

Plasmid

#59554

Purpose

makes 6x Fusion of Full length ceRab21

Depositor

David Lambright

Article

Chotard et al Mol Biol Cell. 2010 Jul 1;21(13):2285-96

Insert

1-207

Tags

6x His

Available Since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only
pET28-ceRab21(1-209)

Plasmid

#59555

Purpose

makes 6x Fusion of Full length ceRab35

Depositor

David Lambright

Article

Chotard et al Mol Biol Cell. 2010 Jul 1;21(13):2285-96

Insert

1-209

Tags

6x His

Available Since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only