We narrowed to 17,559 results for: puromycin
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Plasmid#119188PurposeThe donor (mTFP1) only control for the F40-based human desmoplakin I tension sensor can be used for bleedthrough calibration and provides the donor only lifetime to determine FRET efficiency.DepositorInserthuman Desmoplakin I-[mTFP1-F40-mEYFP(Y67G)] (internal-1945) (DSP Human)
UseTransposonTagsmTFP1-F40-mEYFPExpressionMammalianMutationinserted F40-based tension sensor module after aa…PromoterTCEAvailable SinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin I acceptor only control (F40-based tension sensor)
Plasmid#119189PurposeThe acceptor (mEYFP) only control for the F40-based human desmoplakin I tension sensor can be used for bleedthrough calibration or with the donor only control to determine intermolecular FRET.DepositorInserthuman Desmoplakin I-[mTFP1(Y72G)-F40-mEYFP] (internal-1945) (DSP Human)
UseTransposonTagsmTFP1-F40-mEYFPExpressionMammalianMutationinserted F40-based tension sensor module after aa…PromoterTCEAvailable SinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti CRISPR sgSHMT2_1
Plasmid#106308PurposeExpress Cas9 and sgRNA targeting SHMT2DepositorInsertsgRNA targeting SHMT2
UseCRISPR and LentiviralExpressionMammalianAvailable SinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-Myc-SNAP29
Plasmid#116944PurposeExpressing Myc-SNAP29 for mammalian cellsDepositorInsertsynaptosomal-associated protein, 29kDa (SNAP29 Human)
UseRetroviralTagsMycExpressionMammalianPromoterCMVAvailable SinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-shLacZ-1735
Plasmid#115454PurposeConstitutive lentiviral expressionDepositorInsertshLacZ-1735
UseLentiviral and RNAiExpressionMammalianAvailable SinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-shQKI-3373
Plasmid#115456PurposeConstitutive lentiviral expressionDepositorAvailable SinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti.puro.MCV.ER.RAZ.2
Plasmid#114382PurposeThis lentiviral plasmid expresses MCV tumor T antigens (truncated LT and small T) using the viral NCCR (non-coding control region). The sequence was amplifeied from MCV.R17a and truncated LT=428 aa.DepositorInsertMCV NCCR and tumor T antigen regions
UseLentiviralExpressionMammalianPromoterMCV NCCR (viral own promoter)Available SinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
MIS12-FRB-tagRFP
Plasmid#114039PurposeKinetochore localised FRB tagged with FLAG and tagRFPDepositorAvailable SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-VQRRA-P2A-Puro
Plasmid#110851PurposeLentiviral vector for constitutive expression of Cas9-VQRRA in mammalian cells (codon optimized)DepositorInsertCas9-VQRRA
UseLentiviralTagsFLAGMutationD1135V, R1335Q, T1337R and NLS sequence at the N-…PromoterEF1sAvailable SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRP(Exp)-CMV> ORF_924bp (Azgp1):T2A:dTomato:IRES:Puro
Plasmid#110830PurposeExpresses zinc alpha-2 glycoprotein (ZAG) and dTomato (dT) reporter where both sequences are separated by a T2A self-cleaving peptide sequence as a result, the dT is not fused with the ZAG protein.DepositorAvailable SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX459V2.0-SpCas9-HF4
Plasmid#108295PurposepX459 V2.0 (Plasmid #62988) with the Y450A, N497A, R661A, Q695A, and Q926A mutationsDepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceMay 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti CRISPR sgSHMT1_1
Plasmid#106311PurposeExpress Cas9 and sgRNA targeting SHMT1DepositorInsertsgRNA targeting SHMT1
UseCRISPR and LentiviralExpressionMammalianAvailable SinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
p7127 pHAGE-P-CMVt-N-HA-GAW-FTL
Plasmid#100153PurposeExpresses FTLDepositorAvailable SinceApril 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCT-CMV-UBE3B(C1036A)-copGFP-Puro
Plasmid#107344PurposeExpresses UBE3B mutant C1036A fused to copGFP in mammalian cells.DepositorInsertUBE3B(C1036A)copGFP (UBE3B Human)
UseLentiviralTagscopGFPExpressionMammalianMutationmutation C1036APromoterCMVAvailable SinceApril 19, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMSCVpuro-mApple
Plasmid#96934PurposeRetroviral plasmid containing mApple for labeling of cellsDepositorInsertMonomeric Apple
ExpressionMammalianAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRecLV105
Plasmid#107238PurposeA lentiviral vector for expression in mammalian cellsDepositorTypeEmpty backboneUseRetroviralExpressionMammalianAvailable SinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-MBD3L2
Plasmid#106332PurposeAll-in-one lentivector with tet-inducible MBD3L2DepositorInsertmethyl-CpG binding domain protein 3 like 2 (MBD3L2 Human)
UseLentiviralExpressionMammalianPromotertight TRE promoterAvailable SinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBABE-SSeCKs T751A
Plasmid#107142Purposefull-length murine SSeCKs/AKAP12/Gravin T751A (analogous to T766A mutation in human) in retroviral vectorDepositorInsertSSeCKs T751A (Akap12 Mouse)
UseRetroviralExpressionMammalianMutationchanged threonine 751 to alaninePromotergagAvailable SinceMarch 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCV Puro SCRIB P305L
Plasmid#88887PurposeSCRIB - Proline to Lysine mutation at 305 a.a. in MSCV backboneDepositorInsertSCRIB (SCRIB Human)
UseRetroviralMutationProline to Leucine mutation @ 305th amino acidPromoterMSCV promoterAvailable SinceMarch 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
LEP-shMLL-AF9.1039
Plasmid#105566Purposeretrovirally express MLL-AF9 shRNA with puro resistance and GFP markerDepositorInsertshRNA targeting MLL part of MLL-AF9 fusion
UseRNAi and RetroviralExpressionMammalianPromoterMSCV-LTRAvailable SinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only