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The Addgene repository has many amazing plasmids used by labs all over the world, but what plasmid was request most in 2015?

Addgene reached its 15 year anniversary on January 8, 2019. In this blog post, find the top 15 requested plasmids since our founding. Many of these include lentiviral or CRISPR tools!

Learn how you can visualize newly synthesized proteins with FUNCAT-PLA.

If you’re using CRISPR to make a genome edit, how do you know if your experiment was successful? Try using fluorescent CRISPR reporters like SRIRACCHA and GEmCherry2.

Check out the latest Hot Plasmids, viral vectors, and antibodies in the Addgene repository!

Ready-to-use lentivirus available from Addgene's viral vector packaging service. Browse constructs currently available in lentiviral format.

CRISPR genome editing often proceeds through NHEJ or HDR but microhomology-mediated end-joining (MMEJ) has many benefits - read about them here.

This post describes the design of shuttle vectors, which are designed for replication, selection, and expression in more than one host species.

Addgene lentiviral packaging, envelope, and transfer plasmids

Dr. Le Cong, first author on a seminal CRISPR article from Dr. Feng Zhang's lab, answers FAQs about the genome editing technology.

Lentiviral Vectors deposited by Kazuhiro Oka from the Baylor College of Medicine Gene Vector Core

Addgene's protocol for using the pLKO.1 cloning vector, a backbone used by the RNAi consortium for targeting human and mouse genes.

Learn about retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids.

Find materials created at Addgene, including empty backbones, control vectors, viral packaging and expression plasmids, and more.

Overview of the Sleeping Beauty transposase system and how they are efficient tools for genome engineering.pl

Recent highlights from Addgene's collection include a system to examine biosynthetic pathways, and a new tool for enriching ABE-targeted cells.

Two techniques developed by Addgene depositors have made it possible to track mRNA translation at the single molecule level using fluorescent proteins.

Read about how mammalian plasmids differ from their bacterial counterparts, including how replication occurs and whether selection is necessary for transfected cells.

An easy demethylation protocol uses CRISPR-based dCas9-directed DNA avoids the use of Tet

Gamma-retroviral vectors and lentiviral vectors can be used in a wide variety of experiments including those involving genome engineering, RNAi, and inducible gene expression. In this post, learn about some of the most popular viral vectors available from Addgene and their uses.