Baylor College of Medicine Gene Vector Core Lentiviral and Adenoviral Vectors
Kazuhiro Oka and the (Link opens in a new window) Baylor College of Medicine Gene Vector Core have kindly deposited a series of lentiviral and adenoviral transfer vectors with Addgene.
Lentiviral Transfer Vectors
Within this set of lentiviral transfer vectors you can find reporter plasmids to test whether or not you’ve efficiently generated infectious virus, plasmids expressing both the Cre and Flp recombinases to remove genes flanked by either loxP or frt sites respectively, and empty vectors to express your gene of interest under the control of a variety of promoters. Professor Oka and the Baylor College of Medicine Gene Vector Core have also kindly provided this protocol detailing the process for packaging these transfer vectors and generating infectious virus.
Protocols:
| Lentiviral Vector | ID | Purpose |
|---|---|---|
| pCDH-EF1-DIO-copGFP | 72253 | Expresses copGFP under EF-1 promoter when Cre is expressed by another vector |
| pCDH-EF1-DIO-tdTomato | 72254 | Expresses tdTomato under EF-1 promoter when Cre is expressed by another vector |
| pCDH-CB-iCre-P2A-tdTomato-T2A-Puro | 72255 | NOT AVAILABLE YET Cre is coexpressed with tdTomato and Pac (puromycin N-acethyl-transferase) |
| pCDH-CB-copGFP-T2A-iCre | 72256 | Expresses copGFP and iCre under from the CB promoter |
| pCDH-CB-iCre | 72257 | Express iCre under from the CB promoter |
| pCDH-CB-FLPe-P2A-copGFP-T2A-Puro | 72258 | Expresses FLPe, copGFP and the puromycin resistance from the CB promoter |
| pCDH-CB-FLPe-P2A-tdTomato | 72259 | Expresses FLPe and tdTomato from the CB promoter |
| pCDH-EF1-Fon-copGFP | 72260 | Expresses copGFP from the EF1 promoter when FLP is expressed by another vector |
| pCDH-EF1-Fon-tdTomato | 72261 | Expresses tdTomato from the EF1 promoter when FLP is expressed by another vector |
| pCDH-EF1-FLPe | 72262 | Expresses FLPe from the EF-1 promoter |
| pCDH-EF1-copGFP-T2A-Puro | 72263 | Express copGFP and the puromycin resistance gene from the EF1 promoter |
| pCDH-CMV-mCherry-T2A-Puro | 72264 | Express mCherry and puromycin resistance gene from the CMV promoter |
| pCDH-CMV | 72265 | Express gene of interest from the CMV promoter |
| pCDH-EF1 | 72266 | Express gene of interest from the EF-1 promoter |
| pCDH-CB | 72267 | Express gene of interest from the CB promoter |
| pCDH-PGK | 72268 | xpress gene of interest from the PGK (phosphoglycerate kinase I) promoter |
| pCDH-CMV4 | 72284 | Express gene of interest from the CMV promoter. WPRE has been removed to increase the cloning capacity. |
| pCDH-CB-IRES-copGFP-T2A-Puro | 72299 | Express gene of interest from the CB promoter |
| pCDH-EF1s | 72484 | Express gene of interest from a truncated EF-1 promoter |
| pCDH-EF1-Luc2-P2A-copGFP | 72485 | Expresses Luc2 and copGFP from the EF1 promoter |
| pCDH-EF1-Luc2-P2A-tdTomato | 72486 | Expresses Luc2 and tdTomato from the EF1 promoter |
| pLL3.7-TNFa-shRNA3 | 72598 | Expresses shRNA against mouse TNF-alpha mRNA from the mouse U6 promoter |
| pCDH-EF1-Nluc-P2A-copGFP-T2A-Puro | 73022 | Expresses Nluc and copGFP from the EF1 promoter |
| pCDH-EF1-Nluc | 73024 | Express Nluc from the EF1 promoter |
| pCDH-EF1-copGFP | 73030 | Expresses copGFP from the EF1 promoter |
| pCDH-EF1s-Nluc-P2A-copGFP-T2A-Puro | 73032 | Expresses Nluc and copGFP from a truncated EF1 promoter |
| pCDH-EF1S-Nluc | 73033 | Expresses Nluc from a truncated EF1 promoter |
| pCDH-EF1s-copGFP | 73034 | Expressed copGFP from a truncated EF1 promoter |
| pCDH-CMV-Nluc-P2A-copGFp-T2A-Puro | 73037 | Expresses Nluc and copGFP from the CMV promoter |
| pCDH-CMV-Nluc | 73038 | Expresses Nluc from the CMV promoter |
| pCDH-CB-Nluc-P2A-copGFP-T2A-Puro | 73039 | Expresses Nluc and copGFP from the chicken beta-actin CMV enhancer |
| pCDH-PGK-Nluc-P2A-copGFP-T2A-Puro | 73040 | Expresses Nluc and copGFP from the PGK promoter |
Adenoviral Vectors
The adenoviral transfer vectors contained within this set are derived from pAdenoX from Clontech and can be used to generate adenovirus expressing a variety of reporters as well as the Cre and Flp recombinases. Genes can be cloned into these transfer vectors using shuttle vectors pICPIS-EF1 and pICPIS-CB. pICPIS-EF1 contains the EF1 promoter while pICPIS-CB contains the Chicken-beta actin promoter with CMV enhancer. pAdx-CMV-LacZ is uniquely suited to simplify the cloning process as it can be used in blue-white screening and forms blue colonies on LB/Amp/Xgal/IPTG plates – if your insert is successfully cloned into this vector, transformants will instead form white colonies.
| Adenoviral Vector | ID | Purpose |
|---|---|---|
| pAdx-CMV-LacZ | 73345 | Expresses lacZ from the CMV promoter, forms blue colonies on LB/Amp/Xgal/IPTG plate |
| pAdx-CMV-copGFP | 73346 | Expresses copGFP from the CMV promoter |
| pAdx-CMV-tdTomato | 73347 | Expresses tdTomato from the CMV promoter |
| pAdx-CMV-YFP | 73348 | Express YFP with an NLS from the CMV promoter |
| pAdx-CMV-dsRed | 73349 | Expresses dsRed from the CMV promoter |
| pAdx-CMV-iCre-p2A-copGFP | 73350 | Expresses iCre and EGFP from the CMV promoter |
| pAdx-CMV-iCre-P2A-tdTomato | 73351 | Expresses iCre and tdTomato from the CMV promoter |
| pAdxEF1-FLPe-tdTomato | 73352 | Expresses FLPe and tdTomato from the EF1 promoter |
| pAdx-EF1-copGFP | 73353 | Expresses copGFP from the EF1 promoter |
| pAdx-EF1-tdTomato | 73354 | Expresses tdTomato from the EF1 promoter |
| pICPIS-EF1 | 73355 | Adeno X shuttle vector with EF1 promoter and SV40 polyA |
| pICPIS-CB | 73356 | AdenoX shuttle vector with chicken-beta actin CMV enhancer promoter SV40 polyA |