|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||72284||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 5644
Modifications to backboneClaI/Sal fragment was replace with CMV promoter + new MCS
Vector typeMammalian Expression, Lentiviral
- Promoter CMV promoter
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
Copy numberHigh Copy
- 5′ sequencing primer GGCACCAAAATCAACGGGAC
- 3′ sequencing primer CTGGAATAGCTCAGAGGCCG (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The depositor noted that the single nucleotide mismatch (C>T, bp2193) within the CMV promoter found in Addgene's quality control sequence does NOT affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCDH-CMV4 was a gift from Kazuhiro Oka (Addgene plasmid # 72284 ; http://n2t.net/addgene:72284 ; RRID:Addgene_72284)