We narrowed to 32,805 results for: eng
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Plasmid#227916PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertCAPRIN1 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
UseCRISPRAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML346_VPS35-C-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227891PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertVPS35 homology arms with mEGFP-3xHA for insertion at C terminus
UseCRISPRAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML530_EEA1-N-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227892PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertEEA1 homology arms with mEGFP-3xHA for insertion at N terminus
UseCRISPRAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML348_ATG101-N-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227900PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertATG101 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
UseCRISPRAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML460_MAP1LC3B-N-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227901PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertMAP1LC3B homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
UseCRISPRAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML349_PEX3-C-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227902PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertPEX3 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
UseCRISPRAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML319_SEC31A-N-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227904PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertSEC31A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
UseCRISPRAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML317_COPE-C-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227905PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertCOPE homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
UseCRISPRAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TNT4-Zfr-P2A-HA-luciferase-3xMIR122
Plasmid#223184PurposePlasmid containing the Zfr/Firefly luciferase/3xMIR122 cassette. Translation of luciferase is controlled by splicing modulation of the Zfr specifically in cardiomyocytes by risdiplam in vivo.DepositorInsertsUseAAVTagsHA and engineered P2APromoterTNNT2Available SinceJune 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
Icam 2 SaCas9 YAP sgRNA1
Plasmid#99734PurposeExpresses SaCas9 and a gRNA targeting mouse YAPDepositorAvailable SinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-BrCas12b-FNLDTA
Plasmid#195340PurposeExpresses Engineered BrCas12b in BacteriaDepositorInsertBrCas12b
UseCRISPR and Synthetic BiologyTags6xHis Tag and MBP, TEV cleavage site, 6x His TagExpressionBacterialMutationF208W, N524V, L795I, D868V, T874S, A1015EPromoterT7 PromoterAvailable SinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZE21/UBP1/ClpS_V65I
Plasmid#98567PurposeExpresses truncated codon-opt S. cerevisiae UBP1 and the V65I engineered variant of E. coli ClpS in an artificial operonDepositorInsertsTruncated ubiquitin cleavase, codon optimized
Mutated ATP-dependent Clp protease adapter protein
ExpressionBacterialMutationContains the V65I mutation that increases discrim…Available SinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
Icam 2 SaCas9 YAP sgRNA2
Plasmid#99735PurposeExpresses SaCas9 and a gRNA targeting mouse YAPDepositorAvailable SinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV2 ITR_phU6_gRNA scaffold-DMD sg1_phU6_gRNA scaffold-DMD sg16_pMHCK7-3x Flag-NLS-ecDHFR-enOsCas12f1-NLS-ecDHFR_pA_AAV2 ITR
Plasmid#197029PurposeAAV vector encoding a human codon-optimized ecDHFR-enOsCas12f1-ecDHFR driven by MHCK7 promoter, DMD-targeting guide RNAs compatible with enOsCas12f1 driven by hU6DepositorInserthumanized ecDHFR-enOsCas12f1-ecDHFR
UseAAVMutationD52R / T132RPromoterMHCK7, hU6Available SinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-FBXL5-SKP1
Plasmid#226620PurposeCo-expression of FBXL5-SKP1 complex in E.coliDepositorTagsGB1ExpressionBacterialMutationAmino acids 1-198 was deleted, and 420-596 was re…PromoterT7Available SinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKan cpRBP-FL P53
Plasmid#174504PurposeExpress Full-length P53 in E.coli with cleavable expression/purification tag (ribose binding protein circular permutant)DepositorInsertP53 gene (TP53 Human)
Tags20 amino acid linker containing HRV3C protease si…ExpressionBacterialPromoterT7Available SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLIB_3xFlag-His6-nsp13 (SARS-CoV-2)
Plasmid#169189PurposeBaculoviral transfer vector to express 3xFlag-His6-nsp13 (SARS-CoV-2) in insect cellsDepositorInsert3xFlag-His6-nsp13 (ORF1ab SARS-CoV-2, Synthetic)
Tags3xFlag-His6ExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVRC8400-SARS-CoV-2-S2P-B.1.617.2-AVI
Plasmid#176334PurposeExpresses SARS-CoV-2 S2P protein from the B.1.617.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-S2P-B.1.617.2 (S )
TagsAVI, HRV3C, and scFcExpressionMammalianMutationT19R, G142D, del156-157, R158G, L452R, T478K, D61…Available SinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVRC8400-SARS-CoV-2-RBD-BA.2-AVI
Plasmid#184533PurposeExpresses SARS-CoV-2 RBD domain from the BA.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-RBD-BA.2 (S )
TagsAVI, HRV3C, and scFcExpressionMammalianMutationG339D, S371F, S373P, S375F, T376A, D405N, R408S, …Available SinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVRC8400-SARS-CoV-2-RBD-E484K-AVI
Plasmid#176444PurposeExpresses SARS-CoV-2 RBD domain containing E484K mutation with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorAvailable SinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only