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YjdC-R82A_pET-14b

Plasmid

#233276

Purpose

Overexpress his-tag YjdC protein with a site directed mutagenesis, arginine at position 82 was substituted with alanine (R82A)

Depositor

Mohan Babu, Andrew Emili

Publication

Peng et al Cell. 2025 Jan 22:S0092-8674(25)00032-7.

Insert

YjdC (yjdC )

Expression

Bacterial

Mutation

Arginine was replaced with Alanine at position 82…

Promoter

T7 promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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YjdC-R64A_pET-14b

Plasmid

#233263

Purpose

Overexpress his-tag YjdC protein with a site directed mutagenesis, arginine at position 64 was substituted with alanine (R64A)

Depositor

Mohan Babu, Andrew Emili

Publication

Peng et al Cell. 2025 Jan 22:S0092-8674(25)00032-7.

Insert

YjdC (yjdC )

Expression

Bacterial

Mutation

Arginine was replaced with Alanine at position 64…

Promoter

T7 promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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aTY50_pPGK1_Pcad_IRES_PuroR_WPRE

Plasmid

#225348

Purpose

Lentiviral vector for Pcad expression

Depositor

Wendell Lim

Publication

Yamada et al Cell. 2024 Dec 11:S0092-8674(24)01323-0.

Insert

Pcad (Cdh3 Mouse)

Use

Lentiviral

Expression

Mammalian

Available since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+KDM5B-AS1

Plasmid

#194203

Purpose

Expresses lncRNA KDM5B-AS1

Depositor

Ralf Brandes, Matthias Leisegang

Insert

KDM5B-AS1 (PCAT6 Human)

Expression

Mammalian

Promoter

CMV

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pGWT35S-VC155

Plasmid

#194051

Purpose

Transient expression of VN155 (mVenus β-strands 8 to 11, amino acids 155−239) in plant cell (Cytosol)

Depositor

Yutaka Kodama

Publication

Ichikawa et al ACS Synth Biol. 2022 Oct 21;11(10):3190-

Insert

VC155 (mVenus β-strands 8 to 11, amino acids 155−239)

Expression

Plant

Promoter

CaMV 35S promoter

Available since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pGWT35S-AtOEP7(1-50aa)-mVenus

Plasmid

#194000

Purpose

Transient expression of AtOEP7-mVenus in plant cell (Chloroplast outer envelope membrane)

Depositor

Yutaka Kodama

Publication

Ichikawa et al ACS Synth Biol. 2022 Oct 21;11(10):3190-

Insert

mVenus

Tags

AtOEP7 1–50aa

Expression

Plant

Promoter

CaMV 35S promoter

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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HyWnt3-ΔRep:Luc

Plasmid

#192987

Purpose

Plasmid to test the Hydra Wnt3 promoter activity (full deletion of the repressor element)

Depositor

Brigitte Galliot

Publication

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Hydra Wnt3 promoter

Expression

Mammalian

Mutation

Repressor element deleted

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pvLEA22mer_shuffle_3_mCh-2xFKBP (pBS1119)

Plasmid

#185314

Purpose

For the mammalian expression of the sleeping chironomid protein pvLEA22mer_shuffle_3 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Publication

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

pvLEA22mer_shuffle_3

Expression

Mammalian

Available since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-hFAM3B-HA

Plasmid

#180145

Purpose

Coding sequence of human FAM3B pCS2 with C terminal HA tag

Depositor

Edward De Robertis

Publication

Zhang et al Proc Natl Acad Sci U S A. 2021 May 18;11

Insert

FAM3B (FAM3B Human)

Tags

HA

Expression

Mammalian

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pvLEA22mer_mutant_3_mCh-2xFKBP (pBS1123)

Plasmid

#185318

Purpose

For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_3 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Publication

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

pvLEA22mer_mutant_3

Expression

Mammalian

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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PvLEA4_repeats_k5_1_mCh-2xFKBP (pBS1122)

Plasmid

#185317

Purpose

For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k5_1 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Publication

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

PvLEA4_repeats_k5_1

Expression

Mammalian

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pvLEA22mer_mutant_10_mCh-2xFKBP (pBS1121)

Plasmid

#185316

Purpose

For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_10 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Publication

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

pvLEA22mer_mutant_10

Expression

Mammalian

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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PvLEA4_repeats_k3_26_mCh-2xFKBP (pBS1118)

Plasmid

#185313

Purpose

For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k3_26 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Publication

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

PvLEA4_repeats_k3_26

Expression

Mammalian

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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DHN_VrDHN1a_mCh-2xFKBP (pBS1117)

Plasmid

#185312

Purpose

For the mammalian expression of the riverbank grape plant protein DHN_VrDHN1a attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Publication

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

DHN_VrDHN1a

Expression

Mammalian

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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DHR93_Nccapped_mCh-2xFKBP (pBS1115)

Plasmid

#185310

Purpose

For the mammalian expression of the synthetic protein DHR93_Nccapped attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Publication

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

DHR93_Nccapped

Expression

Mammalian

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pEPQD1CB0867

Plasmid

#177085

Purpose

MoClo Level 1, position 3, transcriptional unit for transient expression of 7-deoxyloganetic acid glucosyl transferase (Cr7-DLGT) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31

Depositor

Nicola Patron

Publication

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

7-deoxyloganetic acid glucosyl transferase (Cr7-DLGT) from Catharanthus roseus

Use

Synthetic Biology

Available since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD1CB0861

Plasmid

#177078

Purpose

MoClo Level 1, position 6, transcriptional unit for transient expression of 7-deoxyloganetic acid glucosyl transferase (Cr7-DLGT) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31

Depositor

Nicola Patron

Publication

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

7-deoxyloganetic acid glucosyl transferase (Cr7-DLGT) from Catharanthus roseus

Use

Synthetic Biology

Available since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_CAPN2_EF-hand

Plasmid

#109858

Purpose

Protein expression and purification of CAPN2_EF-hand

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

CAPN2_EF-hand (CAPN2 Human)

Expression

Bacterial

Available since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_ARPC5_P16-Arc

Plasmid

#109802

Purpose

Protein expression and purification of ARPC5_P16-Arc

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

ARPC5_P16-Arc (ARPC5 Human)

Expression

Bacterial

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pISH-Olfr267

Plasmid

#136754

Purpose

Template to synthesize riboprobes for in situ hybridization (ISH) for Olfr267

Depositor

Peter Mombaerts

Publication

Zapiec et al Cell Rep. 2020 Mar 24;30(12):4220-4234.e

Insert

Olfr267 (Olfr267 Mouse)

Use

In situ probe

Mutation

ISH Probe insert

Available since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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