We narrowed to 9,083 results for: sgRNA

Showing: 6421 - 6440 of 9083 results

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pDrop

Plasmid

#184872

Purpose

Chromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for in-vivo excision of transferred DNA for homologous recombination and counter-selection for successful integration.

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available Since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pRS267

Plasmid

#122380

Purpose

CRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -112 bp upstream of HSP90 start codon

Depositor

Rebecca Shapiro

Article

Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

Insert

HSP90

Use

CRISPR

Expression

Yeast

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pAID-EF1a linearizing in pX330

Plasmid

#140610

Purpose

Crispr/Cas9 plasmid for linearization of pAID-EF1a

Depositor

Tatsuo Fukagawa

Article

Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

Insert

sgRNA for pAID-EF1a plasmid digestion

Use

CRISPR

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pgRNAtet-lvaA

Plasmid

#106397

Purpose

Guide RNA plasmid targeting lvaA on BBR1-UP origin

Depositor

Brian Pfleger

Article

Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

Insert

sgRNA towards lvaA in Pseudomonas putida

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

March 22, 2018

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA KanR neo

Plasmid

#167909

Purpose

Lentiviral vector for expressing U6 sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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PJQ49

Plasmid

#134657

Purpose

Contains Promoter 3 with bsa1 cut sites for inserting sgRNA

Depositor

Jason Peters, Oren Rosenberg

Article

Qu et al J Bacteriol. 2019 Sep 3. pii: JB.00304-1

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

Anderson BBa_J23115

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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PJQ47

Plasmid

#134646

Purpose

Contains Promoter 1 with bsa1 cut sites for inserting sgRNA

Depositor

Jason Peters, Oren Rosenberg

Article

Qu et al J Bacteriol. 2019 Sep 3. pii: JB.00304-1

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

Anderson BBa_J23117

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pMM764

Plasmid

#68901

Purpose

IPTG-inducible CRISPRi vector targeting nonsense sequence (NS), constiutive NanoLuc, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Inserts

dCas9
LacI
sgRNA
NanoLuc

Mutation

L51P

Available Since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pCambia3301-AtU6-MAR1

Plasmid

#210757

Purpose

Mutagenesis of the Arabidopsis MAR1 gene for efficient screening.

Depositor

Daisuke Miki

Article

Cheng et al Sci Rep. 2024 Dec 28;14(1):30791. doi: 1

Insert

AtU6-26 pro::MAR1 sgRNA

Use

CRISPR

Expression

Plant

Promoter

AtU6-26

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pAUX_OL

Plasmid

#166246

Purpose

use as an auxiliary temperature-sensitive plasmid to successfully transform pdCas9_CL plasmid

Depositor

Domitilla Del Vecchio

Article

Huang et al Nat Commun. 2021 Mar 16;12(1):1692. doi:

Insert

[P112-sgRNA-term]-[J23116_B34-dCas9-B15]

Expression

Bacterial

Promoter

Ec-TTL-P112 and BBa_J23116

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCBEclos-cbei2385-g2-opt

Plasmid

#118215

Purpose

CRISPR-mediated base editing in Clostridium

Depositor

Sheng Yang

Article

Li et al Biotechnol Bioeng. 2019 Feb 10. doi: 10.

Insert

Apobec1, Casp nickase, UG1-sgRNA

Expression

Bacterial

Promoter

Ptm

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pdCas9_CL

Plasmid

#166245

Purpose

regulated dCas9 generator

Depositor

Domitilla Del Vecchio

Article

Huang et al Nat Commun. 2021 Mar 16;12(1):1692. doi:

Insert

P112-sgRNA-term-P104-RBS1-dCas9-B15

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ec-TTL-P112 and Ec-TTL-P104

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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KO plasmid

Plasmid

#135970

Purpose

Expresses SpCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Wang et al J Biol Eng. 2019 Apr 24;13:35. doi: 10.1

Type

Empty backbone

Expression

Mammalian

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pAWp28

Plasmid

#73850

Purpose

pBT264-U6p-{2xBbsI}-sgRNA scaffold-{MfeI}

Depositor

Timothy Lu

Article

Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

Type

Empty backbone

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pQdCas9-sgempty

Plasmid

#159523

Purpose

Expression of dCas9 driven by lux cassette from Vibrio fischeri

Depositor

Radhakrishnan Mahadevan

Article

Liu et al ACS Synth Biol. 2020 Jun 5. doi: 10.1021

Insert

quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dcas9 and sgRNA

Use

CRISPR

Promoter

quorum sensing promoter

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-sgTel

Plasmid

#122659

Purpose

Expresses sgRNA for telomere repeats with fluorescent indicator BFP and puromycin selection marker

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

Insert

BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pBLO1811_arC9_noNLS_human

Plasmid

#74494

Purpose

Human codon optimized plasmid to express arC9 t2a mCherry w/o NLS and a sgRNA

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Insert

arC9

Use

CRISPR

Tags

t2a mCherry (C-terminal on insert)

Expression

Mammalian

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgUpf1

Plasmid

#161810

Purpose

guide RNA for Upf1

Depositor

Yi Zhang

Article

Fu et al Sci Adv. 2020 May 29;6(22):eaay5181. doi

Insert

sgRNA targeting mouse Upf1 (Upf1 Synthetic, Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pAbaMCi-GFP-BsaI_pJMP2748

Plasmid

#208888

Purpose

Acinetobacter baumannii CRISPRi test plasmid (GFP, BsaI cloning site in gRNA cassette).

Depositor

Jason Peters

Article

Ward et al mBio. 2023 Dec 21:e0205123. doi: 10.1128

Insert

dCas9, sgRNA expression cassette, sfGFP

Use

Pir dependent origin of replication

Expression

Bacterial

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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GEARBOCS-SPARCL1-N-mCherryTag

Plasmid

#218186

Purpose

To tag Hevin with mCherry at the N-terminal

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Insert

sgRNA (Sparcl1 Mouse)

Use

AAV and CRISPR

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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