We narrowed to 14,728 results for: egf

1. pCMV-T7-ABE8.20m-nSpCas9-NG-P2A-EGFP (KAC1164)

   Plasmid

   #185919

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized ABE8.20m-nSpCas9-NG-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…

   Promoter

   CMV and T7

   Available Since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-hSyn-Gluc-RMA-IRES-EGFP

   Plasmid

   #189629

   Purpose

   Expresses Gluc-RMA and EGFP under the hSyn promoter. Gluc-RMA for monitoring neuronal transduction.

   Depositor

   Jerzy Szablowski

   Article

   Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Inserts

   Gaussia luciferase fused to Fc
   IRES-EGFP
   

   Use

   AAV and Luciferase

   Tags

   Gluc and IgG1 Fc

   Expression

   Mammalian

   Promoter

   IRES and hSyn

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. EGFR(L858R,T790M)-NanoLuc Fusion Vector

   Plasmid

   #237199

   Purpose

   Express EGFR(L858R,T790M)-NanoLuc(R) Fusion Protein in Mammalian Cells under a CMV promoter

   Depositor

   Promega Corporation

   Has Service

   DNA

   Insert

   EGFR (EGFR Human)
   

   Tags

   NanoLuc (R)

   Expression

   Mammalian

   Mutation

   L858R/T790M double mutations

   Promoter

   CMV

   Available Since

   July 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pDisplay-GRAB-rDA3m-IRES-EGFP-CAAX

   Plasmid

   #208696

   Purpose

   Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3m and a membrane-localized EGFP in mammalian cells

   Depositor

   Yulong Li

   Article

   Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   GPCR activation based dopamine (DA) sensor GRAB_rDA3m
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET296-YcpLac111 CYC1p-MCP-NLS-2xyeGFP

   Plasmid

   #104394

   Purpose

   Yeast MCP-GFP expression

   Depositor

   Robert Singer, Evelina Tutucci

   Article

   Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

   Insert

   MCP-NLS-2xyeGFP
   

   Expression

   Bacterial and Yeast

   Mutation

   MCP fused to NLS(SV40) and 2x yeast codon optimiz…

   Promoter

   CYC1

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLenti-CMV-eGFP-hTau P301L-WPRE

   Plasmid

   #233043

   Purpose

   To Express a GFP human Tau P301L fusion protein from a CMV promoter

   Depositor

   Jonathan Ploski

   Insert

   eGFP-hTau P301L fusion (MAPT Synthetic, Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Available Since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSB-CMV-MCS-Puro cPLA2-EGFP

   Plasmid

   #162568

   Purpose

   Used as a measure of cPLA2 activation in live cells with an EGFP tag.

   Depositor

   Philipp Niethammer

   Article

   Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

   Insert

   cytosolic phospholipase a2 (pla2g4aa Zebrafish)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLV UBC hTMEM115-3C-3xHA-P2A-eGFP

   Plasmid

   #214996

   Purpose

   Stably express the GolgiTag (GolgiIP handle) in mammalian cells (human ORF). eGFP for cell sorting desired expression levels.

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   TMEM115 (TMEM115 Human)
   

   Use

   Lentiviral

   Tags

   3xHA

   Expression

   Mammalian

   Promoter

   UBC

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pACTA2_HL-P2A-eGFP-PGK-PuroR-ACTA2_HR

   Plasmid

   #126705

   Purpose

   donor vector for targeting a 2A peptide followed by green fluorescent reporter to the human ACTA2 locus at the end of the endogenous coding sequence

   Depositor

   Darrell Kotton

   Article

   Kwong et al Stem Cell Reports. 2019 Sep 10;13(3):499

   Insert

   GFP
   

   Use

   CRISPR

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLVX-EF1alpha-eGFP-2xStrep-IRES-Puro

    Plasmid

    #141395

    Purpose

    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available Since

    April 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CIRC-V1-Azo-CVB3 IRES-EGFP-BbsI

    Plasmid

    #246757

    Purpose

    RNA circularization with CIRC using Azo intron, harbouring an CVB3 IRES-EGFP reporter.Plasmid was linearized with BbsI.

    Depositor

    Wensheng Wei

    Article

    Shen et al Nat Commun. 2025 Aug 10;16(1):7376. doi:

    Insert

    CIRC-V1-Azo-CVB3 IRES-EGFP-BbsI
    

    Use

    Synthetic Biology

    Available Since

    Oct. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLenti-CMV-eGFP-hTau E14-WPRE

    Plasmid

    #233042

    Purpose

    To Express a GFP human Tau E14 fusion protein from a CMV promoter

    Depositor

    Jonathan Ploski

    Insert

    eGFP-hTau E14 fusion (MAPT Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Available Since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLenti-hSFTPCpromoter(2kb)-EGFP-EF1a-TagRFP

    Plasmid

    #201681

    Purpose

    Lentiviral vector for human SFTPC promoter (2kb)-driven expression of EGFP and EF1a-driven TagRFP expression

    Depositor

    Emma Rawlins

    Article

    Lim et al bioRxiv [Preprint]. 2023 Sep 4:2023.08.3

    Inserts

    SFTPC promoter region (2kb upstream)
    TagRFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. EGFP-SPN_ARR Q37A/R38A/N52R (Shank3)

    Plasmid

    #175256

    Purpose

    SPN-ARR fragment with structure opening N52R and actin binding site Q37A/R38A mutations in EGFP backbone for mammalian expression

    Depositor

    Johanna Ivaska

    Article

    Salomaa et al Curr Biol. 2021 Sep 29. pii: S0960-9822(

    Insert

    SPN-ARR fragment with Q37A/R38A/N52R mutations from SHANK3
    

    Expression

    Mammalian

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PP_079_pAAV_CAG_LR-Voltron2-P2A-CheRiff-eGFP-ST (Kv2.1TS)

    Plasmid

    #230989

    Purpose

    Co-expressing membrane-localized Voltron2 and soma-localized CheRiff.

    Depositor

    Adam Cohen

    Article

    Wong-Campos et al bioRxiv [Preprint]. 2023 May 26:2023.05.

    Insert

    Membrane-localized Voltron2 and soma-localized CheRiff
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPB-EF1A-Puro-hTBK1-E696K-EGFP

    Plasmid

    #221554

    Purpose

    Expresses GFP-tagged human TBK1 with E696K mutation in mammalian cells. Compatible with piggybac transposase for genome integration.

    Depositor

    Shawn Ferguson

    Article

    Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

    Insert

    TBK1 (TBK1 Human)
    

    Use

    Piggybac

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Changed glutamate 696 to lysine

    Promoter

    EF1a

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMMP-MCS-IRES-eGFP-P2A-iCas9

    Plasmid

    #178533

    Purpose

    For genome editing: EGFP-inducible Caspase9 knock-in cassette which allows ablation of target cells

    Depositor

    Ryoji Yao

    Article

    Okamoto et al STAR Protoc. 2021 Sep 16;2(4):100780. do

    Insert

    iCaspase9 (CASP9 Human)
    

    Expression

    Mammalian

    Mutation

    FKBP12 F36 to V Truncated Caspase9 (135-416; lack…

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pShuttle-EGFP-CLASP2 340-1362 9xS/A

    Plasmid

    #24373

    Depositor

    Torsten Wittmann

    Article

    Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

    Insert

    CLASP2 (340-1362) 9xS/A (CLASP2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Nonphosphorylatable CLASP2 deletion mutant. M…

    Available Since

    June 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA5-EGFP-NLS-P2A-mCherry-PTS1

    Plasmid

    #87803

    Purpose

    Mammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using P2A. NLS and PTS1 can be replaced by proteins of interest.

    Depositor

    Andrea Musacchio

    Article

    Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1

    Inserts

    NLS
    PTS1
    

    Tags

    EGFP and mCherry

    Expression

    Mammalian

    Promoter

    CMV promoter, tetracycline operator

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHR-H13LTat-CD8a/d2eGFP-IRES-Nef

    Plasmid

    #126552

    Purpose

    Replication incompetent HIV with H13L Tat and d2eGFP/CD8a reporter

    Depositor

    Jonathan Karn

    Article

    Dobrowolski et al MBio. 2019 Mar 26;10(2). pii: mBio.00337

    Insert

    HIV-1 vector pNL4-3
    

    Use

    Lentiviral

    Tags

    CD8a and GFP

    Mutation

    Delta gag/pol

    Promoter

    HIV LTR

    Available Since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge