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pNLF1-C_WDR41:1-459

Plasmid

#210991

Purpose

Mammalian expression of full-length human WDR41. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR41:M1-V459 (WDR41 Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pFBOH-MHL_CORO1B:1-489

Plasmid

#210906

Purpose

Insect expression of full-length human CORO1B. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

CORO1B:M1-A489 (CORO1B Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_AAMP:82-434

Plasmid

#210904

Purpose

Insect expression of human AAMP fragment D82 to R434. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

AAMP:D82-R434 (AAMP Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_RBBP7:1-411

Plasmid

#210898

Purpose

Insect expression of human RBBP7 fragment M1 to E411. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

RBBP7:M1-E411 (RBBP7 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNLF1-C_MOV10:1-1003

Plasmid

#211086

Purpose

Mammalian expression of full-length human MOV10. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

MOV10:M1-L1003 (MOV10 Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_EIF2D:1-584

Plasmid

#211085

Purpose

Mammalian expression of full-length human EIF2D. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

EIF2D:M1-K584 (EIF2D Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-p1k-Cox4-GFP-APEX2-Ura

Plasmid

#207062

Purpose

Expressing Cox4-GFP-APEX2. GFP interferes with APEX staining.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Cox4

Tags

GFP-APEX2

Expression

Yeast

Promoter

pCOX4

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-pADH3-Snf7-4V5-APEX2-URA

Plasmid

#207065

Purpose

Moderate overexpression of Snf7-4V5-APEX2 under ADH3 promoter. Marker for late endosomes. Only partially functional.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Snf7

Tags

4xV5-APEX2

Expression

Yeast

Promoter

pADH3

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-pERG6-Pex3-4V5-APEX2-URA

Plasmid

#207068

Purpose

Moderate overexpression of Pex3-4V5-APEX2 under ERG6 promoter. Marker for peroxisomes.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Pex3

Tags

4xV5-APEX2

Expression

Yeast

Promoter

pERG6

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-pLSP1-Pex3-4V5-APEX2-URA

Plasmid

#207069

Purpose

Moderate overexpression of Pex3-4V5-APEX2 under LSP1 promoter. Marker for peroxisomes.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Pex3

Tags

4xV5-APEX2

Expression

Yeast

Promoter

pLSP1

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-p1k-Tgl3-4V5-APEX2-URA

Plasmid

#207059

Purpose

Expressing Tgl3-4V5-APEX2. Insufficient signal for EM.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Tgl3

Tags

4xV5-APEX2

Expression

Yeast

Promoter

pTGL3

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-p1k-Pex3-4V5-APEX2-URA

Plasmid

#207057

Purpose

Expressing Pex3-4V5-APEX2. Insufficient signal for EM.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

PEX3

Tags

4xV5-APEX2

Expression

Yeast

Promoter

pPEX3

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-N_RBBP7:1-425

Plasmid

#211020

Purpose

Mammalian expression of full-length human RBBP7. N-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

RBBP7:M1-S425 (RBBP7 Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-N_CCNE1:1-410

Plasmid

#211022

Purpose

Mammalian expression of full-length human CCNE1. N-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

CCNE1:M1-A410 (CCNE1 Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-N_WDR41:1-459

Plasmid

#210990

Purpose

Mammalian expression of full-length human WDR41. N-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR41:M1-V459 (WDR41 Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_RFWD3:1-774

Plasmid

#210955

Purpose

Mammalian expression of full-length human RFWD3. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

RFWD3:M1-E774 (RFWD3 Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_WDR12:1-423

Plasmid

#210953

Purpose

Mammalian expression of full-length human WDR12. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR12:M1-A423 (WDR12 Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-pTPI1-Emc1-GFP-APEX2-Ura

Plasmid

#207074

Purpose

Expressing Emc1-GFP-APEX2. GFP interferes with APEX staining.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Emc1

Tags

GFP-APEX2

Expression

Yeast

Promoter

pTPI1

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-pTPI1-Cox4-GFP-APEX2-Ura

Plasmid

#207075

Purpose

Expressing Cox4-GFP-APEX2. GFP interferes with APEX staining.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Cox4

Tags

GFP-APEX2

Expression

Yeast

Promoter

pTPI1

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pBa.HALO-KIF3A tail 419-702

Plasmid

#193924

Purpose

Mammalian expression of Halo-tagged Kif 3A tail 419-702

Depositor

Marvin Bentley

Article

Garbouchian et al Mol Biol Cell. 2022 Oct 6:mbcE22080336.

Insert

Kif 3A (KIF3A Human)

Tags

Halo

Expression

Mammalian

Promoter

Chicken Beta actin

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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