We narrowed to 9,083 results for: sgRNA

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PX458-mcherry

Plasmid

#161974

Purpose

Expresses Cas9-p2a-mcherry (BbsI site silenced) and sgRNA

Depositor

Joanna Wysocka

Article

Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

Insert

Cas9-t2a-mcherry

Use

CRISPR

Expression

Mammalian

Promoter

Cbh

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pACRISPR

Plasmid

#113348

Purpose

A sgRNA expression plasmid for genome editing in Pseudomonas aeruginosa

Depositor

Quanjiang Ji

Article

Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pNOC-CRISPR

Plasmid

#100008

Purpose

Expresses Cas9-Nlux and sgRNA in Nannochloropsis oceanica, contains hygromycin resistance cassette, for integration into genome

Depositor

Eva Farre

Article

Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

Type

Empty backbone

Use

Algae, nannochloropsis expression

Tags

Cas9-Nlux

Promoter

Ribi promoter

Available Since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pCyCBE

Plasmid

#226505

Purpose

A cytidine deaminase-mediated base-editing plasmid in cyanobacteria

Depositor

Weizhong Chen

Article

Li et al Commun Biol. 2024 Aug 14;7(1):994. doi:

Inserts

APOBEC1-Cas9D10A
sgRNA
sacB gene

Use

CRISPR

Available Since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSGKP-spe

Plasmid

#117234

Purpose

A sgRNA expression plasmid for genome editing in Klebsiella Pneumoniae

Depositor

Quanjiang Ji

Article

Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pHPHS309

Plasmid

#228230

Purpose

GFP dropout backbone for sgRNA library cloning

Depositor

Arvind Rasi Subramaniam

Article

Nugent et al Nat Methods. 2025 Jun;22(6):1237-1246. d

Type

Empty backbone

Expression

Mammalian

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1_Puro_hPGK1_EGFP_Donor

Plasmid

#178088

Purpose

HDR donor plasmid to introduce the EGFP cassette to AAVS1 using AAVS1 T2 sgRNA.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

EGFP

Use

CRISPR

Expression

Mammalian

Promoter

Human PGK1

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR_Puro_sgTP53

Plasmid

#187819

Purpose

pLentiCRISPR that is selectable with puromycin with an sgRNA targeting TP53

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

sgTP53

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pEvolvR-enCas9-PolI3M-TBD

Plasmid

#113077

Purpose

Expresses enCas9 fused to PolI3M-TBD in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

Depositor

John Dueber, David Schaffer

Article

Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

Insert

enCas9-PolI3M-TBD

Expression

Bacterial

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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PB_rtTA_BsmBI

Plasmid

#126028

Purpose

PiggyBac cargo vector expressing rtTA, contains BsmBI sites for sgRNA cloning

Depositor

Mauro Calabrese

Article

Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

Type

Empty backbone

Use

CRISPR; Piggybac

Expression

Mammalian

Promoter

U6

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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pSGKP-km

Plasmid

#117233

Purpose

A sgRNA expression plasmid for genome editing in Klebsiella Pneumoniae

Depositor

Quanjiang Ji

Article

Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pPB_mU6_enh-gRNA_Puro-T2A-BFP

Plasmid

#235571

Purpose

Enhanced guide RNA expression & genomic integration. Target gRNA sequences are cloned in via the BstXI and BlpI sites.

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

sgRNA backbone

Use

CRISPR

Available Since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

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Lenti_gRNA-Puro

Plasmid

#84752

Purpose

Expresses Cpf1 customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter

Depositor

Hyongbum Kim

Article

Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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AIO-mCherry

Plasmid

#74120

Purpose

All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and mCherry-coupled Cas9-D10A nickase to enhance efficient and accurate genome editing

Depositor

Steve Jackson

Article

Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

May 5, 2016

Availability

Academic Institutions and Nonprofits only

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pEvolvR-enCas9-PolI5M

Plasmid

#113078

Purpose

Expresses enCas9 fused to PolI5M in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

Depositor

John Dueber, David Schaffer

Article

Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

Insert

enCas9-PolI5M

Use

CRISPR

Expression

Bacterial

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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AIO-GFP

Plasmid

#74119

Purpose

All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and EGFP-coupled Cas9-D10A nickase to enhance efficient and accurate genome editing

Depositor

Steve Jackson

Article

Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

May 5, 2016

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2 sgSp1

Plasmid

#174907

Purpose

Second generation sgRNA against Sp1

Depositor

Jane Clifford

Article

Swift et al Cell Rep. 2021 Mar 16;34(11):108840. doi

Insert

Specificity Protein 1 (SP1 Human)

Expression

Mammalian

Mutation

Missense mutation to mutate PAM sequence

Promoter

EFS-NS

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pHR: pmU6 sgChr3q29 2xMS2 pUbC MCP-mCherry-p2a-Puro

Plasmid

#174118

Purpose

Expression of sgRNA targeting Chr3q29 (chr3: 195478317 - 195506985, hg38)

Depositor

Stanley Qi

Article

Gao et al Mol Cell. 2021 Aug 13. pii: S1097-2765(2

Insert

sgChr3q29 2xMS2 and MCP-mCherry-p2a-Puro

Use

Lentiviral

Promoter

mU6 and UbC

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pNuc-cis

Plasmid

#202796

Purpose

RK2-based conjugative plasmid containing the TevSpCas9 dual-endonuclease for targeted bacterial killing

Depositor

David Edgell

Article

Hamilton et al Nat Commun. 2019 Oct 4;10(1):4544. doi:

Insert

TevSpCas9, sgRNA cassette, oriT, CenArsHis

Expression

Bacterial

Available Since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-u6-gRNA(deltaD1)-hSyn-mCherry

Plasmid

#231400

Purpose

Knockdown of DRD1 across rodent species

Depositor

Arjen Boender, Larry Young

Article

Karkare et al bioRxiv [Preprint]. 2025 Oct 21:2025.10.

Insert

sgRNA(DRD1.2)

Use

AAV and CRISPR

Expression

Mammalian

Promoter

u6

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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