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YIplac211-AUR1-msGFP2

Plasmid

#177101

Purpose

Expresses Aur1-msGFP2 in yeast cells

Depositor

Benjamin Glick

Publication

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

AUR1 (AUR1 Budding Yeast)

Expression

Yeast

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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YIplac211-RBD2-6xmGFP

Plasmid

#177109

Purpose

Expresses Rbd2-6xmEGFP in yeast cells

Depositor

Benjamin Glick

Publication

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

RBD2 (RBD2 Budding Yeast)

Tags

6xmEGFP

Expression

Yeast

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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YIplac211-SEC2-msGFPx3

Plasmid

#177110

Purpose

Expresses Sec2-msGFPx3 in yeast cells

Depositor

Benjamin Glick

Publication

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

SEC2 (SEC2 Budding Yeast)

Expression

Yeast

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pRS405 VAC8-G2A,C4A,C5A,C7A-RFP

Plasmid

#166842

Purpose

Expresses a myristoylation and palmitoylation mutant Vac8-RFP in yeasts, mutations are Gly2Ala, Cys4Ala, Cys5Ala, Cys7Ala

Depositor

Daniel Klionsky

Publication

Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

Insert

VAC8 (VAC8 Budding Yeast)

Tags

RFP

Expression

Yeast

Mutation

G2A, C4A, C5A, C7A

Promoter

VAC8 promoter

Available since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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pRS405 VAC8-G2A,C4A,C5A,C7A-GFP

Plasmid

#166839

Purpose

Expresses a myristoylation and palmitoylation mutant Vac8-GFP in yeasts, mutations are Gly2Ala, Cys4Ala, Cys5Ala, Cys7Ala

Depositor

Daniel Klionsky

Publication

Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

Insert

VAC8 (VAC8 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

G2A, C4A, C5A, C7A

Promoter

VAC8 promoter

Available since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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pNTI751 pPGK1-GCN4(UTR)-ZEM(TF)

Plasmid

#164916

Purpose

Expresses the ZEM transcription factor under the translational control of the yeast GCN4 5'UTR

Depositor

Nicholas Ingolia

Publication

Muller et al Science. 2020 Dec 11;370(6522). pii: 370

Insert

GCN4(UTR)-ZEM(TF)

Use

Synthetic Biology

Expression

Yeast

Promoter

PGK1 (yeast)

Available since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pAM1160c2

Plasmid

#159724

Purpose

Express MPGIL-G6-Dhfr in yeast two hybrid plasmid, pGBKCg

Depositor

Alexander Varshavsky

Publication

Melnykov et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

Insert

mDHFR (Dhfr Mouse)

Tags

Gal4DBD and MPGIL-GGGGGG

Expression

Yeast

Mutation

remove start and stop codon of Dhfr

Promoter

ADH1

Available since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pAM1674

Plasmid

#159726

Purpose

Express MPTLV-G6-Dhfr in yeast two hybrid plasmid, pGBKCg

Depositor

Alexander Varshavsky

Publication

Melnykov et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

Insert

mDHFR (Dhfr Mouse)

Tags

Gal4DBD and MPTLV-GGGGGG

Expression

Yeast

Mutation

remove start and stop codon of Dhfr

Promoter

ADH1

Available since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAM1479a3

Plasmid

#159725

Purpose

Express MYITV-G6-Dhfr in yeast two hybrid plasmid, pGBKCg

Depositor

Alexander Varshavsky

Publication

Melnykov et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

Insert

mDHFR (Dhfr Mouse)

Tags

Gal4DBD and MYITV-GGGGGG

Expression

Yeast

Mutation

remove start and stop codon of Dhfr

Promoter

ADH1

Available since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCSJ392

Plasmid

#159722

Purpose

Express Gid10 (YGR066C) in yeast two hybrid plasmid, pGADCg

Depositor

Alexander Varshavsky

Publication

Melnykov et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

Insert

GID10 (GID10 Budding Yeast)

Use

Yeast two-hybrid assay

Tags

3xflag and Gal4AD

Expression

Yeast

Mutation

remove stop codon

Promoter

ADH1

Available since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pRS406 ATG9-GFP T119D

Plasmid

#114668

Purpose

Expresses yeast ATG9 (T119D) under the endogenous promoter with a GFP C-terminal tag

Depositor

Daniel Klionsky

Publication

Feng et al Autophagy. 2016;12(4):648-58. doi: 10.10

Insert

ATG9

Tags

GFP

Expression

Yeast

Promoter

ATG9

Available since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pGAD-GH-Mefv iso1

Plasmid

#92107

Purpose

Yeast two hybrid

Depositor

Feng Shao

Publication

Gao et al Proc Natl Acad Sci U S A. 2016 Aug 16;11

Insert

Mefv isoform1 (Mefv Mouse)

Expression

Yeast

Available since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pUG35-YDL183c-GFP

Plasmid

#70161

Purpose

yeast expression of YDL183c with GFP fusion

Depositor

Karin Nowikovsky

Publication

Zotova et al J Biol Chem. 2010 May 7;285(19):14399-41

Insert

YDL183c

Tags

yEGFP

Expression

Yeast

Promoter

MET17

Available since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only
pJK349

Plasmid

#71566

Purpose

Produces Helianthus annuus delta12 fatty acid acetylenase (vFAD2) in yeast

Depositor

T. J. Kappock

Publication

Nam et al J Exp Bot. 2007;58(6):1421-32. Epub 2007

Insert

delta12 fatty acid acetylenase

Expression

Bacterial and Yeast

Promoter

GAL1

Available since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pJK274

Plasmid

#71564

Purpose

Produces Crepis alpina delta12 fatty acid desaturase (FAD2-3) in yeast

Depositor

T. J. Kappock

Publication

Nam et al J Exp Bot. 2007;58(6):1421-32. Epub 2007

Insert

delta12 fatty acid desaturase (form 3)

Expression

Bacterial and Yeast

Promoter

GAL1 promoter

Available since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pJK272

Plasmid

#71562

Purpose

Produces Crepis alpina delta12 fatty acid acetylenase (vFAD2) in yeast

Depositor

T. J. Kappock

Publication

Nam et al J Exp Bot. 2007;58(6):1421-32. Epub 2007

Insert

delta12 fatty acid acetylenase

Expression

Bacterial and Yeast

Promoter

GAL1

Available since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pDS3-X4

Plasmid

#70140

Purpose

Yeast chr. integration vector with DS3 selection marker

Depositor

Morten Sommer

Publication

Rugbjerg et al Metab Eng. 2015 Sep;31:189-97. doi: 10.1

Insert

DS3

Expression

Yeast

Promoter

AgTEF1

Available since

Dec. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pAAH4

Plasmid

#41828

Purpose

Used as a PCR template for tagging yeast proteins with the DHFR tag by homologous recombination and selection for phleomycin resistance

Depositor

Melissa Moore

Publication

Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.

Type

Empty backbone

Use

Pcr template

Expression

Yeast

Promoter

TEF

Available since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pUC-UAS1B11-Leum

Plasmid

#44319

Depositor

Hal Alper

Publication

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B11-Leum promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B11-Leum

Available since

July 15, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pUC-UAS1B1-Leum

Plasmid

#44303

Depositor

Hal Alper

Publication

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B1-Leum promoter

Use

Synthetic Biology

Tags

N/A

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B1-Leum

Available since

July 15, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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