We narrowed to 10,837 results for: yeast
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Plasmid#184973PurposeTest effect of extending a1/a2 on ADE2 editing rates in yeastDepositorInsertEco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 27 v1
ExpressionYeastMutationADE2 donor P272stop, a1/a2 length extended to 27 …PromoterGal7Available sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB329
Plasmid#185077PurposeNUP82 expression in yeastDepositorInsertNUP82
ExpressionYeastAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJo55-CA1
Plasmid#159624PurposePlasmid pJo55, which contains positive control (C. annuum eIF4E-1) for complementation test in Jo55 yeasts.DepositorInsertC. annuum eIF4E-1
ExpressionBacterial and YeastAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ash1
Plasmid#169711PurposeBacterial Expression of yeast Ash1 truncation (encodes amino acids 420-500)DepositorInsertAsh1 (ASH1 Budding Yeast)
Tags6xHisExpressionBacterialMutationTruncation including encoding amino acids 420-500PromoterT7Available sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS405 SEC66-VAC8-GFP
Plasmid#166840PurposeExpresses ER localized Vac8-GFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain.DepositorTagsGFP and SEC66 transmembrane domainExpressionYeastMutationSEC66 transmembrane domainPromoterVAC8 promoterAvailable sinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
CPH3400 (YCp LEU2 Rpb1 A1529_G1534delInsLEVLFQGP)
Plasmid#91806PurposeYeast expression of S. cerevisiae Rpb1 with an internal PreScission protease siteDepositorInsertRPO21 (RPO21 Budding Yeast)
ExpressionYeastMutationResidues A1529-G1534 replaced with a PreScission …PromoterEndogenous Rpb1 promoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CPH3477 (YCp LEU2 Rpb1 P1455_E1456InsLEVLFQGP)
Plasmid#91807PurposeYeast expression of S. cerevisiae Rpb1 with an internal PreScission protease siteDepositorInsertRPO21 (RPO21 Budding Yeast)
ExpressionYeastMutationPreScission Protease site (LEVLFQGP) inserted bet…PromoterEndogenous Rpb1 promoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CPH3486 (YCp LEU2 Rpb1 F1492A)
Plasmid#91808PurposeYeast expression of S. cerevisiae Rpb1 with a F1492A mutationDepositorInsertRPO21 (RPO21 Budding Yeast)
ExpressionYeastMutationchanged phenylalanine 1492 to alaninePromoterEndogenous Rpb1 promoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGST-pol1-N1
Plasmid#160988PurposeExpress a fragment of yeast pol1 proteinDepositorInsertpol1
TagsGSTExpressionBacterialAvailable sinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGST-pol1-N2-2A2
Plasmid#160991PurposeExpress a fragment of yeast pol1 proteinDepositorInsertpol1
TagsGSTExpressionBacterialMutationF58A/D62AAvailable sinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGST-pol1-N2
Plasmid#160989PurposeExpress a fragment of yeast pol1 proteinDepositorInsertpol1
TagsGSTExpressionBacterialAvailable sinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGST-pol1-N1-2A2
Plasmid#160990PurposeExpress a fragment of yeast pol1 proteinDepositorInsertpol1
TagsGSTExpressionBacterialMutationF58A/D62AAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Gal4-RNF169UBD
Plasmid#119013PurposeMammalian expression of fusion protein of yeast Gal4 with human RNF169 Ubiquitin binding domain and BFP expressionDepositorAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
JME5000
Plasmid#129661PurposeEYK1ex_CrisprCas9-yl_RFP: CAS9 vector with EYK1ex marker for gRNA cloning using GoldenGateDepositorTypeEmpty backboneUseCRISPRExpressionYeastAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGS-BacA-21222-FLP-3xFLAG
Plasmid#109165PurposeEncodes yeast full-length Flp with C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression systemDepositorInsertFlp
Tags3xFLAGExpressionInsectPromoterPolyhedrinAvailable sinceApril 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGBDU-Atg17(F317D, I325A)
Plasmid#122275PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
ExpressionYeastMutationPhenylalanine 317 to Aspartic acid; Isoleucine 32…Available sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAD-Atg17(F317H)
Plasmid#122269PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
ExpressionYeastMutationPhenylalanine 317 to HistidineAvailable sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGBDU-Atg17(I325A)
Plasmid#122274PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
ExpressionYeastMutationIsoleucine 325 to alanineAvailable sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAD-Atg17(F317D, I325A)
Plasmid#122273PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
ExpressionYeastMutationPhenylalanine 317 to Aspartic acid; Isoleucine 32…Available sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAD-Atg17(I325A)
Plasmid#122272PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
ExpressionYeastMutationIsoleucine 325 to alanineAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only