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Excess diacylglycerol at the endoplasmic reticulum disrupts endomembrane homeostasis and autophagy.
Li D, Yang SG, He CW, Zhang ZT, Liang Y, Li H, Zhu J, Su X, Gong Q, Xie Z
BMC Biol. 2020 Aug 28;18(1):107. doi: 10.1186/s12915-020-00837-w. PubMed Article

Plasmids from Article

ID Plasmid Purpose
184758OM14-2EGFP-UG74-terMarker for yeast mitochondria. Integration of 2xGFP tag at OM14 C terminus. Uses antibiotic resistance marker natMX6.
184759UG75-DGK1-3HADGK1 overexpression. Promotes the formation of phosphatidate from diacylglycerol. Uses antibiotic resistance marker hphMX6.
184760UG75-CDS1-3HACDS1 overexpression. Diverts glycerolipid synthesis pathway towards phospholipids. Uses antibiotic resistance marker hphMX6.
184761UG75-PAH1-3HAPAH1 overexpression. Promotes the formation of diacylglycerol from phosphatidate. Uses antibiotic resistance marker hphMX6.
184762UG76-pLYS2-GFP-PKCbetaMarker for DAG. Yeast expression of human PKC beta under LSY2 promoter. Uses auxotrophic marker TRP1(Kluyveromyces lactis).
184763EMC1-2EGFP-ter-NATMarker for yeast endoplasmic reticulum (ER). Integration of 2xGFP tag at EMC1 C terminus. Uses antibiotic resistance marker natMX6.
184764SEC7-2EGFP-ter-NATMarker for yeast late Golgi/early endosome . Integration of 2xGFP tag at SEC7 C terminus. Uses antibiotic resistance marker natMX6.
184766IRE1-2EGFP-ter-kanMX4Integration of 2xGFP tag at IRE1 C terminus. Potential indicator of UPR activation. Uses antibiotic resistance marker kanMX4.
184767CIT1-2EGFP-ter-URA3Marker for yeast mitochondria. Integration of 2xGFP tag at CIT1 C terminus. Uses auxotrophic marker URA3(Candida albicans).
184768ABF2-2EGFP-ter-URA3Marker for yeast mitochondria. Integration of 2xGFP tag at ABF2 C terminus. Uses auxotrophic marker URA3(Candida albicans).
184769ACH1-2EGFP-ter-URA3Marker for yeast mitochondria. Integration of 2xGFP tag at ACH1 C terminus. Uses auxotrophic marker URA3(Candida albicans).
184770AFG3-2EGFP-ter-URA3Marker for yeast mitochondria. Integration of 2xGFP tag at AFG3 C terminus. Uses auxotrophic marker URA3(Candida albicans).
184771SYS1-2EGFP-ter-URA3Marker for yeast early Golgi. Integration of 2xGFP tag at SYS1 C terminus. Uses auxotrophic marker URA3(Candida albicans).
184772SEC7-2Katushka-ter-HygromycinRed marker for yeast late Golgi/early endosome. Integration of 2xKatushka tag at SEC7 C terminus. Uses antibiotic resistance marker hphMX6.
184773PKC1-2GFP-ter-TRP1Integration of 2xGFP tag at PKC1 C terminus. Uses auxotrophic marker TRP1(Kluyveromyces lactis).
184774 trp1D-proLYS2-GFP-PKCdelta(C1a+C1b)-NATMarker for DAG. Yeast expression of mouse PKCdelta under LSY2 promoter. Uses antibiotic resistance marker natMX6. Replaces endogenous TRP1 upon genome integration, leading to trp1D.
184775GAL-Aur1-2GFP-HygromycinYeast expression of Aur1 under GAL promoter. Uses antibiotic resistance marker hphMX6.
184776pTPI1-EMC1-GFP-APEX2-URA3Expresses EMC1-GFP-APEX2 chimera for labeling yeast endoplasmic reticulum (ER). Uses auxotrophic marker URA3(Kluyveromyces lactis).
198595UG75-ADH1-DGK1-3HADGK1 overexpression. Promotes the formation of phosphatidate from diacylglycerol. Uses antibiotic resistance marker hphMX6.
198596UG75-ADH1-CDS1-3HACDS1 overexpression. Diverts glycerolipid synthesis pathway towards phospholipids. Uses antibiotic resistance marker hphMX6.
198597UG75-ADH1-PAH1-3HAPAH1 overexpression. Promotes the formation of diacylglycerol from phosphatidate. Uses antibiotic resistance marker hphMX6.

Antibodies from Article