We narrowed to 13,193 results for: lic

1. pEasyG2_mic

   Plasmid

   #184916

   Purpose

   Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_mic recombines in vivo with a PCR product from pEasyG2_zeo/nat/hph.

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMC234r-ccdB/TPK2

   Plasmid

   #176179

   Purpose

   yeast MoClo level-0 part plasmid type 234r containing toxic gene dropout cassette (ccdB and TPK2)

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Insert

   dropout cassette containing toxic genes ccdB and TPK2
   

   Use

   Synthetic Biology

   Available Since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBABE YAP1

   Plasmid

   #15682

   Depositor

   Joan Brugge

   Article

   Overholtzer et al Proc Natl Acad Sci U S A. 2006 Aug 15. 1

   Insert

   YAP (YAP1 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Aug. 31, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. hRAD18-EGFP

   Plasmid

   #68824

   Purpose

   Expresses human RAD18 tagged with EGFP

   Depositor

   Satoshi Tateishi

   Article

   Tsuji et al Genes Cells. 2008 Apr;13(4):343-54. doi:

   Insert

   human RAD18 tagged with EGFP (RAD18 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Oct. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. p5E-mmCdh5pro (JDW 1239)

   Plasmid

   #224478

   Purpose

   Gateway compatible 5' entry clone containing the Murine Cdh5 promoter

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Cdh5 promoter
   

   Expression

   Mammalian

   Available Since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. TDP-43_CTD_M337V

   Plasmid

   #98674

   Purpose

   Expresses 6xHis-tagged C-terminal domain of TDP-43 with M337V

   Depositor

   Nicolas Fawzi

   Article

   Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

   Insert

   TDP-43 (TARDBP Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   M337V

   Available Since

   July 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. CBE-T1.52

   Plasmid

   #193285

   Purpose

   Expression of CBE-T1.52 base editor in mammalian cells, CMV promoter

   Depositor

   Nicole Gaudelli

   Article

   Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

   Insert

   CBE-T1.52
   

   Expression

   Mammalian

   Mutation

   D10A (Cas9), TadA mutations within

   Promoter

   CMV

   Available Since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. HP35 tension sensor

   Plasmid

   #101250

   Purpose

   Expresses the HP35-based tension sensor module. The biosensor allows force measurements at 6-8 pN.

   Depositor

   Carsten Grashoff

   Article

   Austen et al Nat Cell Biol. 2015 Dec;17(12):1597-606.

   Insert

   YPet(short)-HP35-mCherry
   

   Use

   Retroviral

   Tags

   His-tag and cysteine

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBBR_Cas9_sp1

   Plasmid

   #154851

   Purpose

   Expresses SpCas9 and sgRNA that targets the upp gene in Rhodobacter sphaeroides genome

   Depositor

   John van der Oost

   Article

   Mougiakos et al Microb Cell Fact. 2019 Nov 25;18(1):204.

   Inserts

   Spcas9 gene
   sgRNA scaffold
   

   Use

   CRISPR

   Tags

   6xHis

   Mutation

   Codon harmonized variant for expression in Rhodob…

   Available Since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. His-PIMT

    Plasmid

    #34852

    Depositor

    Bruce Downie

    Article

    Dinkins et al Plant J. 2008 Jul;55(1):1-13.

    Insert

    PIMT (PCMT1 Human)
    

    Tags

    6XHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pTRIP-SFFV-GFP-RELA

    Plasmid

    #196629

    Purpose

    Express GFP-RELA

    Depositor

    Nicolas Manel

    Article

    Jeremiah et al J Exp Med. 2023 May 1;220(5):e20220666.

    Insert

    GFP-RELA (RELA Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Mutation

    WT

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFP Cav2.2 HA pCAGGS

    Plasmid

    #206054

    Purpose

    expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and an exofacial double HA tag in domain II

    Depositor

    Annette Dolphin

    Article

    Macabuag et al J Neurosci. 2015 Oct 28;35(43):14636-52.

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV/B-actin

    Available Since

    Oct. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLX303-ABI1-dCas9

    Plasmid

    #154474

    Purpose

    Expresses ABI1 fused to the N terminus of dCas9

    Depositor

    Mikko Taipale

    Article

    Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

    Insert

    ABI1 (ABI1 Mustard Weed)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Includes ABI1 aa 126-423, D143A

    Promoter

    CMV

    Available Since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. CABE-T3.155

    Plasmid

    #193270

    Purpose

    Expression of CABE-T3.155 base editor in mammalian cells, CMV promoter

    Depositor

    Nicole Gaudelli

    Article

    Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

    Insert

    CABE-T3.155
    

    Expression

    Mammalian

    Mutation

    D10A (Cas9), TadA mutations within

    Promoter

    CMV

    Available Since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcs2+spCas9 ABE-Umax-nanos

    Plasmid

    #222142

    Purpose

    Adenine base editor with modified TadA variant for germline A-to-G base editing in zebrafish

    Depositor

    Gaurav Varshney

    Article

    Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

    Insert

    Modified TadA-nSpCas9 with nanos UTR
    

    Use

    CRISPR; Zebrafish expression

    Tags

    Bipartite NLS

    Promoter

    SP6

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-sgRNA(lib)-puro

    Plasmid

    #119976

    Purpose

    Lenti sgRNA cloning backbone with CMV-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Wensheng Wei

    Article

    Liu et al Nat Biotechnol. 2018 Nov 5. pii: nbt.428

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    March 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. p2,0_3M5M_luciferase

    Plasmid

    #135475

    Purpose

    Encodes Marburg virus minigenome with luciferase reporter gene under control of a T7 RNA polymerase promoter

    Depositor

    Elke Mühlberger

    Article

    Manhart et al Cell Rep. 2018 Sep 4;24(10):2573-2580.e4

    Insert

    Marburg virus minigenome, luciferase reporter
    

    Expression

    Mammalian

    Mutation

    None

    Promoter

    T7

    Available Since

    July 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBABEpuro-ARAF

    Plasmid

    #51076

    Purpose

    mammalian expression of wt ARAF

    Depositor

    Matthew Meyerson

    Article

    Imielinski et al J Clin Invest. 2014 Apr;124(4):1582-6. d

    Insert

    ARAF (ARAF Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJK1

    Plasmid

    #111722

    Purpose

    to express human BLM protein in yeast cells

    Depositor

    Ian Hickson

    Article

    Karow et al J Biol Chem. 1997 Dec 5;272(49):30611-4.

    Insert

    BLM (BLM Human)
    

    Tags

    hexahistidine

    Expression

    Yeast

    Mutation

    a C-terminal hexahistidine tag was added, a yeast…

    Promoter

    GAL1

    Available Since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pXL001-TQM IL13 CAR

    Plasmid

    #154054

    Purpose

    Lentiviral expression of TQM IL13 CAR for CAR-T therapy

    Depositor

    Xiaojun Lian

    Article

    Kim et al Bioact Mater. 2020 May 7;5(3):624-635. d

    Insert

    TQM IL13 CAR
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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