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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,083 results for: pET

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Showing: 7101 - 7120 of 10083 results

  1. PIWIL2

    Plasmid
    #32042
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PIWIL2 (PIWIL2 Human)
    Tags
    His (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    PAZ domain only (amino acids 386-533)
    Available since
    Jan. 3, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by

  3. MLH1

    Plasmid
    #28120
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    MLH1:JMC01L-F09:C205690 (MLH1 Human)
    Tags
    His tag with TEV site
    Expression
    Bacterial
    Mutation
    contains amino acid residues 1-347.
    Available since
    March 21, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  4. SRS-MTBD(19/19), AB(monomer), ux

    Plasmid
    #22389
    Depositor
    Ron Vale
    Insert
    Seryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein
    Tags
    His and Myc
    Expression
    Bacterial
    Available since
    Nov. 24, 2009
    Availability
    Academic Institutions and Nonprofits only
    22389-plasmid-map-sequence-id-10296

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  5. SRS-MTBD(22/19), ux, dimer, no myc

    Plasmid
    #22390
    Depositor
    Ron Vale
    Insert
    Seryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein
    Expression
    Bacterial
    Available since
    Nov. 24, 2009
    Availability
    Academic Institutions and Nonprofits only
    22390-plasmid-map-sequence-id-10297

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  6. pZB246-T7-T+

    Plasmid
    #250903
    Purpose
    Expresses N-terminal His-tag thermally stabilized T7 RNAP under a lac-inducible promoter on Kanamycin Resistance Backbone
    Depositor
    Timothy Whitehead
    Publication
    Baumer et al bioRxiv [Preprint]. 2025 Nov 12:2025.11.
    Insert
    Thermally Stable T7 RNA Polymerase
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Arginine 31 to Aspartic Acid, Alanine 61 to Argin…
    Promoter
    pT5-lacUV
    Available since
    Feb. 9, 2026
    Availability
    Academic Institutions and Nonprofits only
    250903-plasmid-map-sequence-id-499452

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  7. pMB-Tl-ADPHK

    Plasmid
    #237469
    Purpose
    Expression of His-tagged Thermococcus litoralis ADP hexokinase
    Depositor
    Michael Baker
    Insert
    ADP hexokinase
    Tags
    C-terminal 10 His-tag and N-terminal strep tag
    Expression
    Bacterial
    Promoter
    T7
    Available since
    June 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    237469-plasmid-map-sequence-id-473992

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  8. His-AgdNK

    Plasmid
    #203226
    Purpose
    For protein expression. A. gambiae deoxynucleoside kinase. Phosphorylates a wide variety of nucleosides at the 5-hydroxyl position. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.
    Depositor
    Vern Schramm
    Publication
    Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi
    Insert
    deoxynucleoside kinase (AgaP_AGAP001585 Anopheles gambiae)
    Tags
    N-terminal TEV cleavable 6xHis tag
    Expression
    Bacterial
    Available since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    203226-plasmid-map-sequence-id-403332

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  9. LT2_14_GlyOx

    Plasmid
    #177711
    Purpose
    Plasmid for genome integration in X2 site expressing GlyRNA and OxPro (biosensors for glycolytic flux and oxidative stress)
    Depositor
    Lisbeth Olsson
    Publication
    Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d
    Inserts
    pTEFmut8-mTurquoise2-RNA_2.6--tCYC1
    pTEFmut8-mCherry-tADH1
    pTRX2_5xUAS-ymYPET-tCYC1
    Use
    Used as donor dna in genome integration after lin…
    Expression
    Yeast
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    177711-plasmid-map-sequence-id-378782

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  11. FEM1C

    Plasmid
    #167842
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    FEM1C (FEM1C Human)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available since
    April 22, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    167842-plasmid-map-sequence-id-331056

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  14. TBXTA-c006

    Plasmid
    #139755
    Purpose
    For protein expression/crystallography (PDB:6F59)
    Depositor
    Opher Gileadi
    Insert
    TBXT, DNA-binding domain, G177D (TBXT Human)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    G177D; contains only amino acids E41-S224
    Promoter
    T7
    Available since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139755-plasmid-map-sequence-id-296509

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  15. 5W9S (CXXC5)

    Plasmid
    #101266
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CXXC5 (CXXC5 Human)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available since
    April 3, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    101266-plasmid-map-sequence-id-193499

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  16. dFP-mini

    Plasmid
    #120543
    Purpose
    Expresses dFP biliverdin-binding fluorescence-activating mini 10 KDa protein in bacteria.
    Depositor
    Brian Chow
    Publication
    Sheehan et al Biochemistry. 2018 Nov 28. doi: 10.1021/
    Insert
    de novo fluorescence-activating protein mini (dFP-mini)
    Tags
    6xHis - TEV cleavage
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Jan. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    120543-plasmid-map-sequence-id-234163

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  17. 6D1T (MBD1)

    Plasmid
    #119966
    Purpose
    Bacterial expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    MBD1 (MBD1 Human)
    Tags
    GSTtag, 6xHIS and Thrombin
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Jan. 8, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    119966-plasmid-map-sequence-id-232690

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  18. pJK650

    Plasmid
    #72400
    Purpose
    Produces Escherichia coli glutamine synthetase with Y397F mutation that prevents adenylation (EcGlnA-Y397F)
    Depositor
    T. J. Kappock
    Insert
    glutamine synthetase (glnA Escherichia coli str. K-12 substr. MG1655))
    Expression
    Bacterial
    Mutation
    Mutated tyrosine-397 to phenylalanine to block ad…
    Promoter
    T7
    Available since
    March 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    72400-plasmid-map-sequence-id-133994

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  19. JMC044-B12

    Plasmid
    #58752
    Purpose
    bacterial expression of PDB 4NW3
    Depositor
    Cheryl Arrowsmith
    Insert
    PDB:4NW3 (KMT2A Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Bacterial expression for structure determination;…
    Promoter
    T7
    Available since
    Aug. 20, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 7101 - 7120 of 10083 results