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pRK5_tAKARα

Plasmid

#119913

Purpose

Mammalian expression of the tAKARα sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

Depositor

Haining Zhong

Article

Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

Insert

tAKARα

Expression

Mammalian

Available Since

March 9, 2019

Availability

Academic Institutions and Nonprofits only

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pKERep

Plasmid

#211826

Purpose

Medium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.

Depositor

Anita Emmerstorfer-Augustin

Article

Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

Insert

eGFP

Expression

Bacterial

Promoter

T5

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pKERep

Plasmid

#211826

Purpose

Medium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.

Depositor

Anita Emmerstorfer-Augustin

Article

Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

Insert

eGFP

Expression

Bacterial

Promoter

T5

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pKESa

Plasmid

#211828

Purpose

Low copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.

Depositor

Anita Emmerstorfer-Augustin

Article

Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

Insert

eGFP

Expression

Bacterial

Promoter

T5

Available Since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pKESa

Plasmid

#211828

Purpose

Low copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.

Depositor

Anita Emmerstorfer-Augustin

Article

Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

Insert

eGFP

Expression

Bacterial

Promoter

T5

Available Since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCPP5060

Plasmid

#128716

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

Depositor

Alan Collmer

Article

Lopez-Solanilla et al Mol Microbiol. 2004 Oct;54(2):353-65. do

Insert

shcN-hopN1

Expression

Bacterial

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCPP5060

Plasmid

#128716

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

Depositor

Alan Collmer

Article

Lopez-Solanilla et al Mol Microbiol. 2004 Oct;54(2):353-65. do

Insert

shcN-hopN1

Expression

Bacterial

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-VAMP2:HRP

Plasmid

#60659

Purpose

Labels synaptic vesicles for electron microscopy

Depositor

Scott Sternson

Article

Atasoy et al Nat Neurosci. 2014 Dec;17(12):1830-9. do

Insert

VAMP2:HRP

Use

AAV and Cre/Lox

Tags

Myc

Promoter

CAG

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
AAV-FLEX-VAMP2:HRP

Plasmid

#60659

Purpose

Labels synaptic vesicles for electron microscopy

Depositor

Scott Sternson

Article

Atasoy et al Nat Neurosci. 2014 Dec;17(12):1830-9. do

Insert

VAMP2:HRP

Use

AAV and Cre/Lox

Tags

Myc

Promoter

CAG

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pHH0103_VDR_Hormone-recep

Plasmid

#109962

Purpose

Protein expression and purification of VDR_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

VDR_Hormone-recep

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_VDR_Hormone-recep

Plasmid

#109962

Purpose

Protein expression and purification of VDR_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

VDR_Hormone-recep

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti6.3-SaCas9-BlastR

Plasmid

#220966

Purpose

Expresses human codon-optimized SaCas9

Depositor

Hyongbum Kim

Article

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

SaCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti6.3-SaCas9-BlastR

Plasmid

#220966

Purpose

Expresses human codon-optimized SaCas9

Depositor

Hyongbum Kim

Article

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

SaCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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STAgR_tdTomato

Plasmid

#102993

Purpose

Can be used as backbone for a STAgR reaction

Depositor

Stefan Stricker

Article

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Type

Empty backbone

Use

CRISPR; Pcr template for stagr vectors

Promoter

U6

Available Since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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STAgR_tdTomato

Plasmid

#102993

Purpose

Can be used as backbone for a STAgR reaction

Depositor

Stefan Stricker

Article

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Type

Empty backbone

Use

CRISPR; Pcr template for stagr vectors

Promoter

U6

Available Since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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pJH1371

Plasmid

#199631

Purpose

Prgef-1 GPI YFP unc-54 3' UTR C.elegans Pan-neural expression of GPI-YFP

Depositor

Mei Zhen

Article

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

GPI

Tags

YFP

Expression

Worm

Promoter

Pregf-1

Available Since

May 7, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pJH1371

Plasmid

#199631

Purpose

Prgef-1 GPI YFP unc-54 3' UTR C.elegans Pan-neural expression of GPI-YFP

Depositor

Mei Zhen

Article

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

GPI

Tags

YFP

Expression

Worm

Promoter

Pregf-1

Available Since

May 7, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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Ki67 (LR)-mNeonGreen-IRESpuro2

Plasmid

#183743

Purpose

Expresses Ki67 (LR)-mNeonGreen

Depositor

Daniel Gerlich

Article

Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

Insert

MKI67 LR domain (MKI67 Human)

Tags

mNeonGreen

Expression

Bacterial and Mammalian

Mutation

LR domain of Ki67

Promoter

CMV

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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Ki67 (LR)-mNeonGreen-IRESpuro2

Plasmid

#183743

Purpose

Expresses Ki67 (LR)-mNeonGreen

Depositor

Daniel Gerlich

Article

Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

Insert

MKI67 LR domain (MKI67 Human)

Tags

mNeonGreen

Expression

Bacterial and Mammalian

Mutation

LR domain of Ki67

Promoter

CMV

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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RnBarr2-mVenus

Plasmid

#137793

Purpose

Visualization of beta-arrestin2

Depositor

Dorus Gadella

Article

Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

Insert

Rat beta-arrestin2 (Arrb2 Rat)

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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