We narrowed to 8,588 results for: Dos

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lentiGuide-puro_Panc480-MT7

Plasmid

#200941

Purpose

Multiplexed CRISPR array expressing 7 sgRNA in a lentiviral backbone

Depositor

James Eshleman

Publication

Teh et al NAR Cancer. 2024 Jun 19;6(2):zcae028. do

Insert

multiplexed sgRNA array

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6, EF-1a

Available since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pJH4293

Plasmid

#200161

Purpose

Ptwk-40s GCaMP6s::mNeptune unc-54 3' UTR C.elegans AVA,AVB and other neurons expression of GCaMP6 mNeptune

Depositor

Mei Zhen

Publication

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

GCaMP6

Tags

mNeptune

Expression

Worm

Promoter

Ptwk-40

Available since

May 7, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pJH4506

Plasmid

#200255

Purpose

Pnpr-4 TeTx mCherry unc-54 3' UTR C.elegans AVA and other neurons expression of TeTx RFP

Depositor

Mei Zhen

Publication

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

TeTx

Tags

mCherry

Expression

Worm

Promoter

Pnpr-4

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pJH4677

Plasmid

#200782

Purpose

Pnpr-4 ins-22::GFP unc-54 3' UTR C.elegans AVA and other neurons expression of ins-22 GFP

Depositor

Mei Zhen

Publication

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

ins-22 (ins-22 Nematode)

Tags

GFP

Expression

Worm

Promoter

Pnpr-4

Available since

May 7, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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2x3APWWP_K299E_D333A-IFP2.0C

Plasmid

#215753

Purpose

Expresses binding-deficient K299E/D333A mutant of 2xDNMT3A PWWP domain fused to C-terminal part of IFP2.0 as negative control for H3K36me2/3 detection in BiAD sensors

Depositor

Albert Jeltsch

Publication

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

2x3APWWP_K299E_D333A-IFP2.0C

Expression

Mammalian

Promoter

CMV

Available since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5-STABLE2-neo-RvCAHS3

Plasmid

#192472

Purpose

Stable expression of RvCAHS3 (fly codon optimized) in Drosophila cells

Depositor

Takekazu Kunieda

Publication

Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

Insert

CAHS3

Tags

T2A-EGFP-T2A-neoR

Expression

Insect

Promoter

Ac5 promoter

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAcGFP1-N1-RvCAHS12-min-GS

Plasmid

#192465

Purpose

Expression of RvCAHS12-min-GS_linker-AcGFP1 in mammalian cells

Depositor

Takekazu Kunieda

Publication

Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

Insert

CAHS12-min

Tags

AcGFP1

Expression

Mammalian

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAcGFP1-N1-RvCAHS3-min-GS

Plasmid

#192463

Purpose

Expression of RvCAHS3-min-GS_linker-AcGFP1 in mammalian cells

Depositor

Takekazu Kunieda

Publication

Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

Insert

CAHS3-min

Tags

AcGFP1

Expression

Mammalian

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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ppil2.280.457.Y389H

Plasmid

#137658

Purpose

Expresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.

Depositor

Tara Davis, Melissa Jurica

Publication

Davis et al PLoS Biol. 2010 Jul 27;8(7):e1000439. do

Insert

ppil2 (PPIL2 Human)

Tags

(His)6-thrombin

Expression

Bacterial

Mutation

280-457-Y389H

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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ppil2.280.457.Y389W

Plasmid

#137657

Purpose

Expresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.

Depositor

Tara Davis, Melissa Jurica

Publication

Davis et al PLoS Biol. 2010 Jul 27;8(7):e1000439. do

Insert

ppil2 (PPIL2 Human)

Tags

(His)6-thrombin

Expression

Bacterial

Mutation

280-457-Y389W

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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KBB438

Plasmid

#185092

Purpose

GAL1::Rfa1[1-337, 344-621]-GFP with NLS removed

Depositor

Ken Belanger

Publication

Belanger et al DNA Cell Biol. 2011 Sep;30(9):641-51. do

Insert

RFA1

Expression

Yeast

Mutation

Rfa1 with amino acids 338-343 deleted

Promoter

GAL1

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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KBB372

Plasmid

#185084

Purpose

GAL::Rfa1[330-621]-GFP under control of GAL1 promoter

Depositor

Ken Belanger

Publication

Belanger et al DNA Cell Biol. 2011 Sep;30(9):641-51. do

Insert

RFA1

Tags

GFP

Expression

Yeast

Mutation

Rfa1 amino acids 330-621

Promoter

GAL1

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-2T-TRR-C421-G2304D

Plasmid

#182845

Purpose

mutation G2304D (GGC/GAC), PCR product coding C-terminal residues 2011-2431 of TRR was inserted into modified pGEX-2T by Nde I-Nsi I sites

Depositor

Peter Harte

Publication

Tie et al Development. 2014 Mar;141(5):1129-39. do

Insert

TRR 2011-2431 (trr Fly)

Tags

GST

Expression

Bacterial

Mutation

mutation G2304D (GGC/GAC)

Available since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pLyGo-Ec-4

Plasmid

#163130

Purpose

pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PhoA signal peptide and the LyGo cassette (SapI-ccdB-SapI)

Depositor

Morten Norholm

Publication

Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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pLyGo-Ec-3

Plasmid

#163129

Purpose

pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding OmpA signal peptide and the LyGo cassette (SapI-ccdB-SapI)

Depositor

Morten Norholm

Publication

Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available since

May 6, 2021

Availability

Academic Institutions and Nonprofits only

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p35S_EVDdi

Plasmid

#167120

Purpose

Plant binary expression vector containing the coding sequence of the Arabidopsis Copia93 retroelement EVADE (AT5G17125) lacking the intron under CaMV 35S promoter.

Depositor

Arturo Marí-Ordóñez, Olivier Voinnet

Publication

Oberlin et al Genome Res. 2017 Sep;27(9):1549-1562. do

Insert

EVADE (EVD) (AT5G17125 Mustard Weed)

Expression

Plant

Mutation

Deleted intron from full length element

Promoter

CaMV 35S

Available since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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p35S_EVDwt

Plasmid

#167119

Purpose

Plant binary expression vector containing the coding sequence of the Arabidopsis Copia93 retroelement EVADE (AT5G17125) under CaMV 35S promoter.

Depositor

Arturo Marí-Ordóñez, Olivier Voinnet

Publication

Oberlin et al Genome Res. 2017 Sep;27(9):1549-1562. do

Insert

EVADE (EVD) (AT5G17125 Mustard Weed)

Expression

Plant

Promoter

CaMV 35S

Available since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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pLyGo-Ec-2

Plasmid

#163128

Purpose

pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding MalE signal peptide and the LyGo cassette (SapI-ccdB-SapI)

Depositor

Morten Norholm

Publication

Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available since

April 28, 2021

Availability

Academic Institutions and Nonprofits only

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pLyGo-Ec-5

Plasmid

#163131

Purpose

pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding DsBA signal peptide and the LyGo cassette (SapI-ccdB-SapI)

Depositor

Morten Norholm

Publication

Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCAT.334

Plasmid

#119556

Purpose

Level T acceptor vector for chromosomal integration with lacZ compatible with blue/white screening. Modified pUC19 vector.

Depositor

Alistair McCormick

Publication

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Type

Empty backbone

Use

Synthetic Biology

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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