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JAB2494

Plasmid

#190376

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::RpaR-VP16_19St)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proG1090::RpaR-VP16_19St

Expression

Plant

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-NHERF1 3xSA

Plasmid

#195207

Purpose

Mammalian expression vector for GFP tagged NHERF1. Triple PKC site phosphorylation-deficient mutant (S162/339/340A)

Depositor

Anthony Bretscher, David Bryant

Article

Garbett et al J Cell Biol. 2010 Oct 18;191(2):397-413.

Insert

NHERF1 (NHERF1 Human)

Expression

Mammalian

Mutation

S162/339/340A

Available Since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-NHERF1 3xSD

Plasmid

#195208

Purpose

Mammalian expression vector for GFP tagged NHERF1. Triple PKC site phosphomimetic mutant (S162/339/340D)

Depositor

Anthony Bretscher, David Bryant

Article

Garbett et al J Cell Biol. 2010 Oct 18;191(2):397-413.

Insert

NHERF1 (NHERF1 Human)

Expression

Mammalian

Mutation

S162/339/340D

Available Since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-NHERF1 PDZ1/2*

Plasmid

#195211

Purpose

Mammalian expression vector for GFP tagged NHERF1. Dual PDZ1/2 GYGF>GYAA motif mutant

Depositor

Anthony Bretscher, David Bryant

Article

Garbett et al J Cell Biol. 2010 Oct 18;191(2):397-413.

Insert

NHERF1 (NHERF1 Human)

Expression

Mammalian

Mutation

Dual PDZ1/2 GYGF>GYAA motif mutant

Available Since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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pJMH020

Plasmid

#186633

Purpose

contains homology arms for the pilA (NIES39_C03030) locus of Arthrospira platensis

Depositor

Adam Arkin

Article

Hilzinger et al Biotechnol Bioeng. 2025 Jan;122(1):44-52

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pJMH021

Plasmid

#186634

Purpose

contains homology arms for the NSI (NIES39_Q01230) locus of Arthrospira platensis

Depositor

Adam Arkin

Article

Hilzinger et al Biotechnol Bioeng. 2025 Jan;122(1):44-52

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pJMH022

Plasmid

#186635

Purpose

contains homology arms for the oscillin (NIES39_A01430) locus of Arthrospira platensis

Depositor

Adam Arkin

Article

Hilzinger et al Biotechnol Bioeng. 2025 Jan;122(1):44-52

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pJMH059

Plasmid

#186641

Purpose

aadA.co-TaadA-PrbcL-eYFP.co between homology arms for pilA

Depositor

Adam Arkin

Article

Hilzinger et al Biotechnol Bioeng. 2025 Jan;122(1):44-52

Insert

aadA.co-TaadA-PrbcL-eYFP.co

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pJMH140

Plasmid

#186643

Purpose

aadA.co-TaadA-PJ23111-eYFP.co between homology arms for pilA

Depositor

Adam Arkin

Article

Hilzinger et al Biotechnol Bioeng. 2025 Jan;122(1):44-52

Insert

aadA.co-TaadA-PJ23111-eYFP.co

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pJMH141

Plasmid

#186644

Purpose

aadA.co-TaadA-PJ23100-eYFP.co between homology arms for pilA

Depositor

Adam Arkin

Article

Hilzinger et al Biotechnol Bioeng. 2025 Jan;122(1):44-52

Insert

aadA.co-TaadA-PJ23100-eYFP.co

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pJMH142

Plasmid

#186645

Purpose

aadA.co-TaadA-PJ23107-eYFP.co between homology arms for pilA

Depositor

Adam Arkin

Article

Hilzinger et al Biotechnol Bioeng. 2025 Jan;122(1):44-52

Insert

aadA.co-TaadA-PJ23107-eYFP.co

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pMPRAduo:∆orf

Plasmid

#193740

Purpose

MPRAduo backbone

Depositor

Ryan Tewhey

Article

Mouri et al Cell Genom. 2022 Dec 16;3(1):100234. doi

Type

Empty backbone

Expression

Mammalian

Promoter

none

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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hPCNA-S228I

Plasmid

#190938

Purpose

untagged human PCNA with a hypomorphic disease-associated serine to isoleucine mutation at residue 228

Depositor

Brian Kelch

Article

Duffy et al J Mol Biol. 2016 Mar 27;428(6):1023-1040

Insert

Proliferating Cell Nuclear Antigen (PCNA Human)

Expression

Bacterial

Mutation

changed serine 228 to isoleucine

Promoter

T7

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-GST-DmNanos_116-163-E151AF152AN155A-V5His6_AC

Plasmid

#148466

Purpose

Insect Expression of DmNanos_116-163-E151AF152AN1555A

Depositor

Elisa Izaurralde

Article

Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

Insert

DmNanos_116-163-E151AF152AN1555A (nos Fly)

Expression

Insect

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pnEA-NpG-DmNanos-opt_116-163-F152E_AB

Plasmid

#148332

Purpose

Bacterial Expression of DmNanos-opt_116-163-F152E

Depositor

Elisa Izaurralde

Article

Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

Insert

DmNanos-opt_116-163-F152E (nos Fly)

Expression

Bacterial

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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mILK-R349A-EFGP

Plasmid

#176898

Purpose

mouse ILK with R349A point mutation cloned into pEGFP-N1

Depositor

Sara Wickström

Article

Martin et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

Insert

Integrin-linked kinase (Ilk Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Arginine 349 to Alanine

Promoter

CNV

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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mILK-R255A-EFGP

Plasmid

#176897

Purpose

mouse ILK with R255A point mutation cloned into pEGFP-N1

Depositor

Sara Wickström

Article

Martin et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

Insert

Integrin-linked kinase (Ilk Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Arginine 255 to Alanine

Promoter

CNV

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pET19b-rDAD2 cPGFP(N180)

Plasmid

#166001

Purpose

Expression of coding sequence in Escherichia coli

Depositor

Claudia Vickers

Article

Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

Insert

rDAD2 cPGFP(N180)

Expression

Bacterial

Mutation

LSSmOrange- V49A (please see depositor comments)

Promoter

T7 promoter

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pET19b-DAD2 cPGFP(A149)

Plasmid

#165998

Purpose

Expression of coding sequence in Escherichia coli

Depositor

Claudia Vickers

Article

Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

Insert

DAD2 cPGFP(A149)

Expression

Bacterial

Promoter

T7 promoter

Available Since

May 3, 2021

Availability

Academic Institutions and Nonprofits only

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pET19b-rShHTL7 cPGFP(E167)

Plasmid

#166004

Purpose

Expression of coding sequence in Escherichia coli

Depositor

Claudia Vickers

Article

Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

Insert

rShHTL7 cPGFP(E167)

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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