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CMV-10-3xFLAG-LGP2-MI

Plasmid

#58682

Purpose

Expresses LGP2 with a mutation to motif I in mammalian cells

Depositor

Curt Horvath

Article

Bamming et al J Biol Chem. 2009 Apr 10;284(15):9700-12

Insert

LGP2-MI (DHX58 Human)

Tags

3x FLAG

Expression

Mammalian

Mutation

Lysine 30 mutated to Alanine

Promoter

CMV

Available Since

July 31, 2014

Availability

Academic Institutions and Nonprofits only
pSP64-hERG1a S620T

Plasmid

#53059

Purpose

Expresses alpha subunit of S620T hERG1a K channel in Xenopus oocytes

Depositor

Michael Sanguinetti

Article

Casis et al Mol Pharmacol. 2006 Feb;69(2):658-65. Ep

Insert

Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)

Use

Xenopus oocyte expression

Mutation

changed Serine 620 to Threonine

Promoter

SP6

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

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pCin4-3xFLAG-MKL1 L555A

Plasmid

#19851

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

Insert

MKL1 L555A (MRTFA Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Leucine 555 to Alanine

Available Since

Feb. 12, 2009

Availability

Academic Institutions and Nonprofits only
integrin beta6 777T pBS

Plasmid

#13581

Depositor

Dean Sheppard

Article

Morris et al Nature. 2003 Mar 13. 422(6928):169-73.

Insert

integrin beta 6 777T (ITGB6 Human)

Expression

Bacterial

Mutation

777T. Lacks last 11 amino acids of cytoplasmic do…

Available Since

Jan. 8, 2007

Availability

Academic Institutions and Nonprofits only

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CaV1.3e[8a,11,31b,Δ32,42a]

Plasmid

#49333

Purpose

Repaired Addgene #26576 to agree with the rat ref sequence. Repairs are @: nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].

Depositor

Diane Lipscombe

Article

Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

Insert

CACNA1D (Cacna1d Rat)

Expression

Mammalian

Mutation

Contains exons 8a, 11, 31b and 42a.Deletion of ex…

Promoter

CMV

Available Since

Feb. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pDisplay-DNER

Plasmid

#51053

Purpose

expression of HA-DNER in mammalian cells

Depositor

Mineko Kengaku

Article

Eiraku et al J Biol Chem. 2002 Jul 12;277(28):25400-7

Insert

DNER (Dner Mouse)

Tags

HA and signal peptide

Expression

Mammalian

Mutation

without endogenous signal peptide (1-221bp)

Promoter

CMV

Available Since

Feb. 19, 2014

Availability

Academic Institutions and Nonprofits only
10279-M28-663

Plasmid

#225662

Purpose

Lentivirus protein expression. Matched control construct for 8522-M01-663 (Addgene # 225660) gives basal expression from mCMVp for gating/thresholding

Depositor

Dominic Esposito

Article

Tang et al Stem Cell Reports. 2015 Jan 13;4(1):155-

Insert

dsCopGFP

Use

Lentiviral

Promoter

mCMVp

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAJ075_hsCRBN

Plasmid

#124214

Purpose

Insect cell expression vector for His6 tagged hsCRBN

Depositor

Eric Fischer

Article

Nowak et al Nat Chem Biol. 2018 Jul;14(7):706-714. d

Insert

Cereblon (CRBN Human)

Tags

His6-TEV

Expression

Insect

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pSBbi-FoxO1_1R_10A_3D

Plasmid

#106278

Purpose

Fluorescent fusion protein for FoxO1

Depositor

Laura Heiser

Article

Gross et al Cell Syst. 2019 Dec 18;9(6):580-588.e4.

Inserts

Foxo1a (Foxo1 Mouse)
NLS-mCherry-NLS-P2A-Puromycin

Tags

Clover fluorescent protein

Expression

Mammalian

Mutation

Deleted amino acids 401-636, S209A, H212R, S215A,…

Promoter

EF1a/RPBSA

Available Since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLEX-MCS-ASC-GFP

Plasmid

#73957

Purpose

Express human ASC with a C-terminal EGFP tag in mammalian cells

Depositor

Christian Stehlik

Article

de Almeida et al Immunity. 2015 Aug 18;43(2):264-76. doi:

Insert

PYD and CARD domain containing, PYCARD (PYCARD Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

CMV IE

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-AID-BLM-UGI-T2A-mTagBFP2

Plasmid

#221222

Purpose

Express HACE Editor as a fusion of AID cytidine deaminase and BLM helicase with mTagBFP2 reporter

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

AID-BLM-UGI-T2A-mTagBFP2

Expression

Mammalian

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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LIC1B_Caspase-LOVN7C-7

Plasmid

#104629

Purpose

bacterial expression of light-activated caspase-3 with N7C-7 linker variation

Depositor

Jim Wells

Article

Smart et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

Insert

Caspase-3 (CASP3 Human)

Tags

His and LOV2

Expression

Bacterial

Promoter

T7

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-DR(RfxCas13d)-CAG-hfCas13d-pa

Plasmid

#233036

Purpose

To express a RfxCas13d-compatible gRNA and to express HA Tagged hfCas13d from a CAG promoter

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

RfxCas13d-compatible gRNA expression cassette and hfCas13d

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-AID-PcrA M6-UGI-T2A-mTagBFP2

Plasmid

#221225

Purpose

Express HACE Editor as a fusion of AID cytidine deaminase and an optimized PcrA M6 helicase with mTagBFP2 reporter

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

AID-PcrA M6-UGI-T2A-mTagBFP2

Expression

Mammalian

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pMGF182

Plasmid

#97006

Purpose

Expresses GFP-CHMP7 in mammalian cells under a crippled promoter

Depositor

Adam Frost, Wesley Sundquist

Article

Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

Insert

CHMP7 (CHMP7 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV delta 5

Available Since

Aug. 14, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti-MAPK14

Plasmid

#200973

Purpose

a mammalian expression vector to express MAPK14

Depositor

Jason Sheltzer

Article

Bhattacharjee et al Cell Chem Biol. 2023 Oct 19;30(10):1211-

Insert

mitogen-activated protein kinase 14 (MAPK14) (MAPK14 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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tet-TadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA

Plasmid

#221235

Purpose

Tet-inducible lentiviral HACE Editor as a fusion of TadA-8e adenosine deaminase and an optimized PcrA M6 helicase.

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

TadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA

Use

Lentiviral

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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10279-M30-663

Plasmid

#225663

Purpose

Lentivirus protein expression. Matched control construct for 8522-M31-663 (Addgene # 225661) gives basal expression from mCMVp for gating/thresholding

Depositor

Dominic Esposito

Article

Tang et al Stem Cell Reports. 2015 Jan 13;4(1):155-

Insert

dsCherry

Use

Lentiviral

Promoter

mCMVp

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-Ins-TRE3Gp-dCas9-Suntag-VP64-EF1Ap-Puro

Plasmid

#183409

Purpose

piggyBAC-based doxycycline-inducible dCas9-5xSuntag-VP64 CRISPR activation plasmid for enhancer and promoter activation in mammalian cells

Depositor

Azim Surani

Article

Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

Insert

dCas9-5xGCN5-P2A-scFV-sfGFP-VP64-GB1

Use

CRISPR and Synthetic Biology; Piggybac

Expression

Mammalian

Promoter

TRE3G

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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EphA2-YFP

Plasmid

#108852

Purpose

Encodes for human EphA2 fluorescently labeled with eYFP on the C-Terminus via a 15 amino acid (GGS)5 flexible linker

Depositor

Kalina Hristova

Article

Singh et al J Biol Chem. 2015 Nov 6;290(45):27271-9.

Insert

EPHA2 (EPHA2 Human)

Tags

Labeled with eYFP on the C-Terminus via a 15 amin…

Expression

Mammalian

Available Since

April 11, 2018

Availability

Academic Institutions and Nonprofits only

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