We narrowed to 13,119 results for: LIC

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p2lox-cAMPr R626A

Plasmid

#102437

Purpose

cAMPr with a point mutation R626A which theoretically decreases its affinity for cAMP

Depositor

Justin Blau

Article

Hackley et al Sci Signal. 2018 Mar 6;11(520). pii: 11/

Insert

cAMPr

Use

Cre/Lox

Expression

Mammalian

Mutation

PKA-R R626A

Available Since

Feb. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pCMVTnT-Flp-3xFLAG

Plasmid

#109167

Purpose

Encodes yeast full-length Flp with C-terminal 3xFLAG tag to be expressed in a mammalian expression system

Depositor

Monika Golas

Article

Jensen et al Protein J. 2017 Aug;36(4):332-342. doi:

Insert

Flp

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pSE360-Prp22

Plasmid

#111284

Purpose

Prp22 WT gene in pSE360 backbone

Depositor

Christine Guthrie

Article

Noble et al Genetics. 1996 May;143(1):67-80.

Insert

PRP22

Expression

Yeast

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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Sub2 pRS315

Plasmid

#111300

Purpose

Sub2 in pRS315 vector

Depositor

Christine Guthrie

Article

Kistler et al Genes Dev. 2001 Jan 1;15(1):42-9.

Insert

SUB2

Expression

Yeast

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pLSLT

Plasmid

#51498

Purpose

pLSL with T30 TALEN followed by us:NPTII repair template, Rep is not included on this plasmid

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Inserts

T30 TALEN pair
us:NPTII repair template

Use

Plant t-dna plasmid

Promoter

virion-sense LIR and 2x35S

Available Since

May 1, 2014

Availability

Academic Institutions and Nonprofits only

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pSKDuet23

Plasmid

#41697

Depositor

Hideo Iwai

Article

Ellila et al FEBS Lett. 2011 Oct 10.

Insert

PhoPol-II intein

Tags

GB1 and His-tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pSKDuet22

Plasmid

#41696

Depositor

Hideo Iwai

Article

Ellila et al FEBS Lett. 2011 Oct 10.

Insert

PhoCDC21-2 intein

Tags

GB1 and His-tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pSKDuet21

Plasmid

#41695

Depositor

Hideo Iwai

Article

Ellila et al FEBS Lett. 2011 Oct 10.

Insert

PhoCDC21-1 intein

Tags

GB1 and His-tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pSKB3.TM1777

Plasmid

#11421

Depositor

Sung-Hou Kim

Article

Shin et al Biochemistry. 2003 Nov 25. 42(46):13429-

Insert

NusA (nusA T. maritima)

Tags

6x His and TEV protease site

Expression

Bacterial

Available Since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
mCherry-C1-ORP5deltaTMD-FKBP

Plasmid

#220073

Purpose

expresses ORP5 with the transmembrane domain deleted. The mCherry fluorophore and FKBP are attached to ORP5.

Depositor

Gerry Hammond

Article

Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

Insert

OSBPL5deltaTMD (OSBPL5 Human)

Expression

Mammalian

Mutation

deltaTMD

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-MPRA-MluI-SpeI-EcoRI

Plasmid

#190196

Purpose

Empty vector for inserting MPRA libraries into AAV

Depositor

Hyejung Won

Article

McAfee et al Cell Genom. 2023 Sep 15;3(10):100404. do

Type

Empty backbone

Use

AAV; Massively parallel reporter assay (mpra)

Expression

Mammalian

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_ASCC3_GRIK2

Plasmid

#205774

Purpose

Express mEGFP-tagged fusion protein, ASCC3_GRIK2 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

ASCC3_GRIK2 (ASCC3, GRIK2 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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enz.105-MelA

Plasmid

#154049

Purpose

Expresses MelA in E. coli

Depositor

Christopher Voigt

Article

Wallace et al Advanced Functional Materials 2020

Insert

Tyrosinase MelA

Tags

His, MBP

Expression

Bacterial

Promoter

T7

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pJPF0117

Plasmid

#130267

Purpose

Lentiviral vector for the co-expression of cytoplasmic bPAC and nuclear tagBFP

Depositor

Hana El-Samad

Article

Fonseca et al bioRxiv 2020, 747030

Insert

bPAC

Use

Lentiviral

Tags

tagBFP

Expression

Mammalian

Promoter

hPGK

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMG343

Plasmid

#176183

Purpose

preassembled yeast MoClo level-1 backbone with toxic gene dropout cassette (ccdB and TPK2) and URA3 marker

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

TPK2+ccdB dropout cassette

Expression

Yeast

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pLBS.EF/mScarlet(i)-hCdt100Nt

Plasmid

#129334

Purpose

Cell cycle reporter, lentivirus

Depositor

Yasushi Okada

Article

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

mScarlet(i) (Addgene 85044)

Use

Lentiviral

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pTargeting_Mettl3C_FKBP-V

Plasmid

#165421

Purpose

Template for knockin FBKP-V into Mettl3 C-terminus

Depositor

Neil Brockdorff

Article

Wei et al Genome Res. 2021 Aug;31(8):1395-1408. do

Insert

Mettl3 C-terminus + linker + FKBP-V (Mettl3 Mouse)

Use

Mouse Targeting

Tags

FKBP-V

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pGEMHE-POLArISact

Plasmid

#164970

Purpose

T7 promotor drives in vitro transcription of POLArISact with a human kozak sequence

Depositor

Sumio Terada

Article

Sugizaki et al Proc Natl Acad Sci U S A. 2021 Mar 16;11

Insert

POLArISact

Use

In vitro transcription

Promoter

T7

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCFJ2214 - Intron GG1 - 900 bp

Plasmid

#159880

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron GG1 - 900 bp

Expression

Worm

Mutation

Not applicable

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pTKA002

Plasmid

#164578

Purpose

Expresses SARS-CoV-2 nsp8 from a pET46 backbone for IPTG-inducible bacterial expression

Depositor

Robert Kirchdoerfer

Article

Altincekic et al Front Mol Biosci. 2021 May 10;8:653148.

Insert

nsp8

Tags

6xHis, Ek, TEV

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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