We narrowed to 14,951 results for: pET

1. pDest17_Gcn4 ILVtoED W

   Plasmid

   #231871

   Purpose

   Bacterial expression of N-terminally 6His tagged Gcn4 ILVtoED W

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 ILVtoED W
   

   Use

   Tags

   6xHis, TEV cleavage site

   Expression

   Bacterial

   Mutation

   N126W, I128E, V130E, V135E, L137D, I142D

   Promoter

   T7

   Available since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDest17_Gcn4 ILVtoAS

   Plasmid

   #231859

   Purpose

   Bacterial expression of N-terminally 6His tagged Gcn4 ILVtoAS

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 ILVtoAS
   

   Use

   Tags

   6xHis, TEV cleavage site

   Expression

   Bacterial

   Mutation

   L123S, I128S, V130A, V135A, L137S, I142S

   Promoter

   T7

   Available since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDest17_Gcn4 ILVtoED

   Plasmid

   #231857

   Purpose

   Bacterial expression of N-terminally 6His tagged Gcn4 ILVtoED

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 ILVtoED
   

   Use

   Tags

   6xHis, TEV cleavage site

   Expression

   Bacterial

   Mutation

   L123D, I128E, V130E, V135E, L137D, I142D

   Promoter

   T7

   Available since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGGA016

   Plasmid

   #228231

   Purpose

   Level 0 for 5xOp (GreenGate A module)

   Depositor

   Alexis Maizel

   Article

   Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

   Insert

   5xOp
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGGC018

   Plasmid

   #228232

   Purpose

   Level 0 for LhG4 (GreenGate C module)

   Depositor

   Alexis Maizel

   Article

   Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

   Insert

   GR-LhG4
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   -

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJB67

   Plasmid

   #226316

   Purpose

   E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

   Depositor

   David Savage

   Article

   Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

   Insert

   CsoS2 with K to A CsoS2 MR1-6
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   K261A, K320A, K380A, K436A, K495A, K546A

   Promoter

   pTRC

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJB68

   Plasmid

   #226315

   Purpose

   E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

   Depositor

   David Savage

   Article

   Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

   Insert

   CsoS2 with Y to A CsoS2 MR1-7
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Y300A, Y359A, Y420A, Y475A, Y534A, Y585A, Y644A

   Promoter

   pTRC

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJB74

   Plasmid

   #226314

   Purpose

   E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

   Depositor

   David Savage

   Article

   Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

   Insert

   CsoS2 with Y to A CsoS2 MR1-5
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Y300A, Y359A, Y420A, Y475A, Y534A

   Promoter

   pTRC

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJB73

   Plasmid

   #226313

   Purpose

   E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

   Depositor

   David Savage

   Article

   Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

   Insert

   CsoS2 with Y to A CsoS2 MR1-3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Y300A, Y359A, Y420A

   Promoter

   pTRC

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJB51

    Plasmid

    #226308

    Purpose

    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

    Depositor

    David Savage

    Article

    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

    Insert

    CsoS2 with V(T/S)G to AAA CsoS2 MR1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    VTG273-275AAA, VTG284-286AAA, VTG295-297AAA

    Promoter

    pTRC

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJB48

    Plasmid

    #226307

    Purpose

    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

    Depositor

    David Savage

    Article

    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

    Insert

    CsoS2 with C to S CsoS2 MR1-6
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C292S, C310S, C351S, C369S, C467S, C485S, C526S, …

    Promoter

    pTRC

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJB47

    Plasmid

    #226306

    Purpose

    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

    Depositor

    David Savage

    Article

    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

    Insert

    CsoS2 with C to S CsoS2 MR1-5
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C292S, C310S, C351S, C369S, C467S, C485S, C526S, …

    Promoter

    pTRC

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJB46

    Plasmid

    #226305

    Purpose

    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

    Depositor

    David Savage

    Article

    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

    Insert

    CsoS2 with C to S CsoS2 MR1-4
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C292S, C310S, C351S, C369S, C467S, C485S

    Promoter

    pTRC

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJB45

    Plasmid

    #226304

    Purpose

    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

    Depositor

    David Savage

    Article

    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

    Insert

    CsoS2 with C to S CsoS2 MR1-2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C292S, C310S, C351S, C369S

    Promoter

    pTRC

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAMC070n1a12

    Plasmid

    #209746

    Purpose

    ColA BBa_B0014 tetAp SD8 ScTrpRS rrnB1 proB SD8 Lum1PylRS BBaB10014RNAItrpA+ J23100 SD8 MmPylRS T3Te-T7Te DHFR

    Depositor

    Ahmed Badran

    Article

    Costello et al Nat Biotechnol. 2024 Sep 11. doi: 10.103

    Insert

    tRNA operon - ScTrpRS - Lum1PylRS - MmPylRS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MUNC13-1 MUN

    Plasmid

    #218889

    Purpose

    Expresses the MUN domain from MUNC13-1 in bacteria

    Depositor

    Axel Brunger

    Article

    Leitz et al Nat Protoc. 2024 Jul 2. doi: 10.1038/s41

    Insert

    MUNC13-1 MUN domain (Unc13a Rat)
    

    Use

    Tags

    6 his

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. C1C2CMUNC2C

    Plasmid

    #218674

    Purpose

    Insect cell expression of a rat MUNC13-1 fragment containing domains C1C2BMUNC2C

    Depositor

    Axel Brunger

    Article

    Leitz et al Nat Protoc. 2024 Jul 2. doi: 10.1038/s41

    Insert

    MUNC13 (Unc13a Rat)
    

    Use

    Tags

    GST

    Expression

    Insect

    Mutation

    Amino acids1407-1453 deleted

    Promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHB1

    Plasmid

    #204668

    Purpose

    pUC19 containing the LL-FRT-erm-FRT-spacer sequence which serves as template for BarSeq deletion constructs

    Depositor

    Mark Mandel

    Article

    Burgos et al mSystems. 2020 Dec 15;5(6):e00846-20. do

    Insert

    FRT-erm-FRT
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMP065

    Plasmid

    #207929

    Purpose

    Premade plasma membrane marker, pUBQ10:PiP1;4:mVenus:tHSP18.2M, for braiding

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMP086

    Plasmid

    #207930

    Purpose

    Premade plasma membrane marker, pUBQ10:PiP1;4:mVenus:tHSP18.2M:FastRed, for braiding

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge