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pBSV2G_PflaB-msfYFPBb

Plasmid

#118233

Purpose

Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven msfYFP

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

flagellin promoter driven msfYFP

Expression

Bacterial

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pDisplay-Gncp-iGluSnFR

Plasmid

#107337

Purpose

Noncircularly permutated (ncp) variant of iGluSnFR. Membrane-displayed.

Depositor

Robert Campbell

Article

Wu et al ACS Chem Biol. 2018 Jan 22. doi: 10.1021

Insert

GltI based and noncircularly permutated glutamate biosensor

Expression

Mammalian

Promoter

CMV

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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p5E-CMVmin

Plasmid

#80802

Purpose

minimal cytomegalovirus immediate early enhancer/promoter (CMVmin) from pcDNA3

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

minimal cytomegalovirus immediate early enhancer/promoter

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pCMVTnT-Flp-3xFLAG

Plasmid

#109167

Purpose

Encodes yeast full-length Flp with C-terminal 3xFLAG tag to be expressed in a mammalian expression system

Depositor

Monika Golas

Article

Jensen et al Protein J. 2017 Aug;36(4):332-342. doi:

Insert

Flp

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pBAD-R-GECO1.2

Plasmid

#113937

Purpose

Bacterial expression of red fluorescent Ca2+ sensor R-GECO1.2

Depositor

Robert Campbell, Thomas Hughes

Article

Molina et al Biophys J. 2019 May 21;116(10):1873-1886

Insert

R-GECO1.2

Tags

6xHis

Expression

Bacterial

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pGBW-m4137386

Plasmid

#149540

Purpose

Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 S (surface glycoprotein) (S Human, Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits
pLRD16

Plasmid

#208268

Purpose

Production of syn-stemar-13-ene in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
osCPS4
osKSL8

Expression

Bacterial

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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Fus-BcLOV-mCh

Plasmid

#208277

Purpose

Expresses Fus-BcLOV fusion for optogenetic membrane recruitment. Includes an mCherry tag.

Depositor

Lukasz Bugaj

Article

Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

Insert

Fus-BcLOV-mCh

Expression

Mammalian

Promoter

pCMV

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMA3442

Plasmid

#184852

Purpose

Retroviral vector encoding puromycin resistance and Cre recombinase

Depositor

Mikhail Alexeyev

Article

Kozhukhar et al Cells. 2022 Jul 11;11(14). pii: cells111

Insert

Cre

Use

Cre/Lox and Retroviral

Tags

Myc-tag

Expression

Mammalian

Promoter

LTR

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pBSV2G_PflaB-mCeruleanBb

Plasmid

#118235

Purpose

Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven mCerulean

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

flagellin promoter driven mCerulean

Expression

Bacterial

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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hBax S184E C3-EGFP

Plasmid

#19743

Depositor

Richard Youle

Article

Nechushtan et al EMBO J. 1999 May 4. 18(9):2330-41.

Insert

Bax S184E (BAX Human)

Tags

GFP

Expression

Mammalian

Mutation

changed Serine 184 to Glutamate

Available Since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only
pCCL-ReDNAP (Y427A)

Plasmid

#52346

Purpose

CEN6/ARS4 plasmid, REV1 promoter>Recoded TP-DNAP1 (Y427A)

Depositor

Chang Liu

Article

Ravikumar et al Nat Chem Biol. 2014 Mar;10(3):175-7. doi

Insert

Recoded TP-DNAP

Expression

Bacterial and Yeast

Promoter

REV1

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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CMVTCRa_candidate10

Plasmid

#165007

Purpose

Expression of candidate TCR #10 TCRalpha cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

TRAV (TRA Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pNP1 sfGFP trigger

Plasmid

#107359

Purpose

Trigger sequence for pNP1 sfGFP toehold switch

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

toehold trigger 7

Expression

Bacterial

Promoter

T7

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-Ace2_D92N_E199V-mNeon-ST

Plasmid

#129261

Purpose

Express soma-localized Ace2_D92N_E199V-mNeon in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Ace2_D92N_E199V-mNeon-ST

Expression

Mammalian

Mutation

D92N, E199V

Promoter

CAG

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pLJM1-PCYT2

Plasmid

#81074

Purpose

Expresses PCYT2 in mammalian cells

Depositor

Erin O'Shea

Article

Chidley et al Elife. 2016 Jul 12;5. pii: e14601. doi:

Insert

phosphate cytidylyltransferase 2, ethanolamine (PCYT2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pN-PITCh-HF

Plasmid

#127882

Purpose

Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter H3F tagging

Depositor

Peter Kaiser

Article

Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

Insert

GFP-Puro-H3F

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pRSETB-ro2GFP

Plasmid

#82366

Purpose

Inducible expression of redox sensitive GFP in bacteria

Depositor

S. James Remington

Article

Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

Insert

ro2GFP

Tags

6xHIS

Expression

Bacterial

Mutation

C48S/S65T/Q80R/S147C/Q204C

Promoter

T7

Available Since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pMCSGC-GB1

Plasmid

#186786

Purpose

E. coli expression vector containing N-terminal 6His-GB1-TEV fusion.

Depositor

Alexei Savchenko

Article

Venkatesan et al iScience. 2022 Sep 3;25(10):105054. doi:

Type

Empty backbone

Expression

Bacterial

Promoter

T7

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pN-PITCh-H3FAID

Plasmid

#127884

Purpose

Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter H3FAID tagging

Depositor

Peter Kaiser

Article

Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

Insert

GFP-Puro-H3F-AID

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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