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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 15,730 results for: LEA

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Showing: 7441 - 7460 of 15730 results

  3. pHL718

    Plasmid
    #62826
    Purpose
    Lim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + PLtetO-1::ipex::mCherry::T1 terminator + ColE1
    Depositor
    Han Lim
    Publication
    Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv
    Inserts
    gfp
    mCherry
    Use
    Synthetic Biology
    Tags
    ipex leader and ompC leader
    Expression
    Bacterial
    Promoter
    pLlacO-1 and pLtetO-1
    Available since
    Aug. 14, 2015
    Availability
    Academic Institutions and Nonprofits only

  8. pcDNA3-mCherry-TSC2(AA,3Q)-NLS

    Plasmid
    #192445
    Purpose
    Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A, K1614Q, K1615Q, R1616Q mutations to disrupt Akt phosphorylation and GAP activity; targeted to the nucleus.
    Depositor
    Jin Zhang
    Publication
    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry-TSC2(S939A,T1439A,K1614Q,K1615Q,R1616Q )-NLS (TSC2 Human)
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …
    Promoter
    CMV
    Available since
    Dec. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    192445-plasmid-map-sequence-id-379137

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  11. pCVL TAL(Nd154C63) SFFV Age-Xho/Xba-Sal linkers

    Plasmid
    #50421
    Purpose
    pTAL plasmid for cloning of megaTALs or other TAL-fusions and expression in mammalian cells. RVDs are cloned between Age-Xho sites and meganuclease/fusion is cloned between Xba-Sal sites.
    Depositor
    Andrew Scharenberg
    Publication
    Boissel et al Nucleic Acids Res. 2013 Nov 26.
    Type
    Empty backbone
    Use
    Lentiviral and TALEN
    Expression
    Mammalian
    Promoter
    SFFV
    Available since
    May 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    50421-plasmid-map-sequence-id-79593

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  20. pST50Trc1-FLAGHISNDHFR

    Plasmid
    #63947
    Purpose
    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Publication
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    6xHis & FLAG; TEV cleavable
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    63947-plasmid-map-sequence-id-177457

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Showing: 7441 - 7460 of 15730 results