We narrowed to 8,708 results for: sgRNA

1. pJMP1360

   Plasmid

   #119278

   Purpose

   for cloning new sgRNAs

   Depositor

   Oren Rosenberg, Carol Gross, Jason Peters

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   dcas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Promoter

   Available since

   Jan. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. SPDK3889(TRV-RNA2-pPEBV-MCS-AtMet-tRNA)

   Plasmid

   #149277

   Purpose

   Modified Tobacco rattle virus RNA2 vector that could be used for expression of sgRNAs

   Depositor

   Savithramma Dinesh-Kumar

   Article

   Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

   Insert

   Modified TRV RNA2 with PEBV promoter and AtMet-tRNA mobile RNA sequence
   

   Use

   T-dna vector

   Tags

   Expression

   Mutation

   T2318C (DNA) in PEBV coat protein sequence

   Promoter

   Available since

   June 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6-sgGAL4-1

   Plasmid

   #46915

   Purpose

   Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GAL4 UAS promoter

   Depositor

   Jonathan Weissman, Stanley Qi

   Article

   Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

   Inserts

   sgGAL4-1
   Puromycin resistance and mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. Cas12f-GE ver3.0

   Plasmid

   #176508

   Purpose

   Expresses Cas12f-GE in mammalian cells

   Depositor

   Yong-Sam Kim

   Article

   Kim et al Nat Biotechnol. 2021 Sep 2. pii: 10.1038

   Inserts

   Cas12f-GE ver3.0
   Cas12f-GE ver3.0
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and chicken β-actin

   Available since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGM79

   Plasmid

   #220171

   Purpose

   CRISRPi guide RNA against MARCHF5

   Depositor

   Jonathan Weissman

   Article

   Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

   Insert

   MARCHF5 (sgRNA) (MARCHF5 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pODN-mR2-ACTB

   Plasmid

   #183868

   Purpose

   Repair template for the N-terminal tagging of beta-actin with mRuby2 in human cells using CRISPR/Cas9.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   ACTB homology arms with mRuby2-linker (ACTB Human)
   

   Use

   CRISPR; Donor template

   Tags

   mRuby2-linker

   Expression

   Mammalian

   Mutation

   Homology arms contain point mutations to remove t…

   Promoter

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX458_Ruby

   Plasmid

   #110164

   Purpose

   Cas9 from S. pyogenes with Ruby2, and cloning backbone for sgRNA

   Depositor

   Philip Hublitz

   Article

   Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and Ruby2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. plenti-px330-CRBN-T2-pGK-Pur

   Plasmid

   #107383

   Purpose

   Mammalian expression CRISPR/Cas9

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   sgRNA targeting CRBN (CRBN Human)
   

   Use

   Lentiviral; Crispr

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSECC-sgTom

   Plasmid

   #138661

   Purpose

   Expresses a Tomato-targeting sgRNA, Cas9, and Cre-recombinase

   Depositor

   Reuben Shaw

   Article

   Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

   Insert

   sgTomato
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENTR221-H1-sgGFP1-U6-sgGFP2-7SK-sgGFP3

    Plasmid

    #87906

    Purpose

    sgRNA targeting GFP

    Depositor

    Nicole Déglon

    Article

    Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

    Insert

    sgGFP1, sgGFP2, sgGFP3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1, U6, 7SK

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. iMAP-61

    Plasmid

    #187460

    Purpose

    gRNA array of iMAP-61

    Depositor

    Tian Chi

    Article

    Liu et al Cell. 2022 Aug 4;185(16):3008-3024.e16.

    Insert

    sgRNA array
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    modified U6

    Available since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMonAID_dCas9-PmCDA_Hyg_ALS

    Plasmid

    #91692

    Purpose

    Monocot Target-AID vector expressing rice-optimized dCas9-PmCDA1 with sgRNA targeting OsAls

    Depositor

    Akihiko Kondo

    Article

    Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    SpCas9
    

    Use

    Tags

    PmCDA1

    Expression

    Plant

    Mutation

    D10A and H840A for dead Cas9

    Promoter

    2x35S

    Available since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. qTAG-N-Blast-ultraID-LMNB1

    Plasmid

    #207775

    Purpose

    Donor template for Blast-2A-ultraID insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    LMNB1 Homology Arms flanking a Blast-ultraID Cassette (LMNB1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pLV-hIP-dCas9-BSD

    Plasmid

    #183231

    Purpose

    Lentiviral vectors with the human insulin promoter driving expression of dCas9, in addition to a U6-driven sgRNA

    Depositor

    Jorge Ferrer

    Article

    Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Human insulin promoter

    Available since

    Oct. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJMP1221

    Plasmid

    #119260

    Purpose

    rfp "test" strain

    Depositor

    Oren Rosenberg, Carol Gross, Jason Peters

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA NT1/RR1 (rfp)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. Direct-seq_lentiGuide-Puro-tRNA-Loop2-8A8G

    Plasmid

    #157986

    Purpose

    Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

    Depositor

    Lijia Ma

    Article

    Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

    Insert

    S. pyogenes sgRNA cassette (tRNA-Loop2-8A8G)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEJS2603_AAV-Nme2-ABE8e-i1-U6-Rosa26

    Plasmid

    #201685

    Purpose

    All-in-one AAV plasmid expressing Nme2-ABE8e-i1 and U6 driven sgRNA targeting the mouse Rosa26 gene

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

    Insert

    Nme2-ABE8e-i1
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U1a

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEJS2604_AAV-Nme2-ABE8e-i1(V106W)-U6-Rosa26

    Plasmid

    #201686

    Purpose

    All-in-one AAV plasmid expressing Nme2-ABE8e-i1 (V106W) and U6 driven sgRNA targeting the mouse Rosa26 gene

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

    Insert

    Nme2-ABE8e-i1 (V106W)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U1a

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330 Ctnnb1.2

    Plasmid

    #59912

    Purpose

    pX330 backbone expressing sgRNA targeting Ctnnb1 to edit mouse beta-Catenin. Expresses Cas9 from CBh promoter

    Depositor

    Tyler Jacks

    Article

    Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1

    Insert

    Ctnnb1 (Ctnnb1 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJMP1104

    Plasmid

    #119248

    Purpose

    knockdown pqsC in P. aeruginosa

    Depositor

    Oren Rosenberg, Carol Gross, Jason Peters

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA pqsC (P. aeruginosa)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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