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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 1,029 results for: gibson assembly

Showing: 741 - 760 of 1029 results

  1. GFP-MIOX only

    Plasmid
    #166683
    Purpose
    Yeast integrative plasmid for expressing mouse myo-inositol oxygenase fused to GFP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-
    Insert
    GFP-MIOX
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL10
    Available since
    April 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    166683-plasmid-map-sequence-id-329938

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  2. pTEF1-UDH

    Plasmid
    #166679
    Purpose
    Yeast integrative plasmid for expressing P. syringae uronate dehydrogenase, with TEF1 promoter. Contains leucine auxotrophic marker (K. lactis LEU2).
    Depositor
    Claudia Vickers
    Article
    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-
    Insert
    UDH
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF1
    Available since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    166679-plasmid-map-sequence-id-329863

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  3. wtVP1 only

    Plasmid
    #166673
    Purpose
    Yeast integrative plasmid for expressing wild-type Murine polyomavirus VP1, with GAL1 promoter. Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-
    Insert
    Wild-type Murine polyomavirus VP1
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1
    Available since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    166673-plasmid-map-sequence-id-329825

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  7. pmCherry_I3

    Plasmid
    #239340
    Purpose
    Expresses the I3-01 nanocage subunit N-terminally tagged with mCherry in mammalian cells. Assembles into nanocages tagged with 60 FPs.
    Depositor
    Torsten Wittmann
    Insert
    I3-01
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    K129A
    Promoter
    CMV
    Available since
    June 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    239340-plasmid-map-sequence-id-474987

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  8. pI3_EGFP

    Plasmid
    #239335
    Purpose
    Expresses the I3-01 nanocage subunit C-terminally tagged with EGFP in mammalian cells. Assembles into nanocages tagged with 60 EGFPs.
    Depositor
    Torsten Wittmann
    Insert
    I3-01
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    K129A
    Promoter
    CMV
    Available since
    June 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    239335-plasmid-map-sequence-id-474985

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  9. pmStayGold_mStayGold_I3

    Plasmid
    #239339
    Purpose
    Expresses the I3-01 nanocage subunit N-terminally tagged with two copies of mStayGold in mammalian cells. Assembles into nanocages tagged with 120 FPs.
    Depositor
    Torsten Wittmann
    Insert
    I3-01
    Use
    Tags
    mStayGold
    Expression
    Mammalian
    Mutation
    K129A
    Promoter
    CMV
    Available since
    June 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    239339-plasmid-map-sequence-id-474988

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  10. pmBaoJin_I3

    Plasmid
    #239337
    Purpose
    Expresses the I3-01 nanocage subunit N-terminally tagged with mBaoJin in mammalian cells. Assembles into nanocages tagged with 60 FPs.
    Depositor
    Torsten Wittmann
    Insert
    I3-01
    Use
    Tags
    mBaoJin
    Expression
    Mammalian
    Mutation
    K129A
    Promoter
    CMV
    Available since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    239337-plasmid-map-sequence-id-474360

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  11. pmStayGold_I3

    Plasmid
    #239336
    Purpose
    Expresses the I3-01 nanocage subunit N-terminally tagged with mStayGold in mammalian cells. Assembles into nanocages tagged with 60 FPs.
    Depositor
    Torsten Wittmann
    Insert
    I3-01
    Use
    Tags
    mStayGold
    Expression
    Mammalian
    Mutation
    K129A
    Promoter
    CMV
    Available since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    239336-plasmid-map-sequence-id-474358

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  12. pmScarlet3_I3

    Plasmid
    #239342
    Purpose
    Expresses the I3-01 nanocage subunit N-terminally tagged with mScarlet3 in mammalian cells. Assembles into nanocages tagged with 60 FPs.
    Depositor
    Torsten Wittmann
    Insert
    I3-01
    Use
    Tags
    mScarlet3
    Expression
    Mammalian
    Mutation
    K129A
    Promoter
    CMV
    Available since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    239342-plasmid-map-sequence-id-474359

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  13. pmRuby3_I3

    Plasmid
    #239344
    Purpose
    Expresses the I3-01 nanocage subunit N-terminally tagged with mRuby3 in mammalian cells. Assembles into nanocages tagged with 60 FPs.
    Depositor
    Torsten Wittmann
    Insert
    I3-01
    Use
    Tags
    mRuby3
    Expression
    Mammalian
    Mutation
    K129A
    Promoter
    CMV
    Available since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    239344-plasmid-map-sequence-id-474342

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  14. pYZ033

    Plasmid
    #98404
    Purpose
    Entry vector for S. pombe CRISPR-Cas9 system. The gRNA can be integrated easily through Gibson Assembly with the plasmid backbone digested by Not1
    Depositor
    Jef Boeke
    Article
    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    98404-plasmid-map-sequence-id-198860

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Showing: 741 - 760 of 1029 results