We narrowed to 1,068 results for: gibson assembly

1. pBN215

   Plasmid

   #167962

   Purpose

   express E. coli E69 (O9a:K30) Wzc with Y715E, Y717E, Y718E

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   E. coli E69 (O9a:K30) Wzc with Y715E, Y717E, Y718E
   

   Use

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   Y715E, Y717E, Y718E

   Promoter

   araBAD

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBN216

   Plasmid

   #167963

   Purpose

   express E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E, Y718E

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E, Y718E
   

   Use

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   Y713E, Y715E, Y717E, Y718E

   Promoter

   araBAD

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBN217

   Plasmid

   #167964

   Purpose

   express E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E, Y718E

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E, Y718E
   

   Use

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   Y708E, Y713E, Y715E, Y717E, Y718E

   Promoter

   araBAD

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBN261

   Plasmid

   #167966

   Purpose

   express E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E
   

   Use

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   Y713E, Y715E, Y717E

   Promoter

   araBAD

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBN262

   Plasmid

   #167967

   Purpose

   express E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E
   

   Use

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   Y708E, Y713E, Y715E, Y717E

   Promoter

   araBAD

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBN008

   Plasmid

   #167949

   Purpose

   express E. coli E69 (O9a:K30) Wzc with K540M mutation

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30), K540M nutant
   

   Use

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   K540M

   Promoter

   araBAD

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBN202

   Plasmid

   #167950

   Purpose

   express E. coli E69 (O9a:K30) Wzc_K540M with Y713E, Y715E, Y717E, Y718E mutations

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   E. coli E69 (O9a:K30) Wzc_K540M with Y713E, Y715E, Y717E, Y718E mutations
   

   Use

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   K540M, Y713E, Y715E, Y717E, Y718E

   Promoter

   araBAD

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBN093

   Plasmid

   #167951

   Purpose

   express E. coli E69 (O9a:K30) Wzc_K540M with periplasmic motif 3 truncation

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   E. coli E69 (O9a:K30) Wzc_K540M with periplasmic motif 3 truncation
   

   Use

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   K540M, delete motif 3

   Promoter

   araBAD

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GFP-MIOX only

   Plasmid

   #166683

   Purpose

   Yeast integrative plasmid for expressing mouse myo-inositol oxygenase fused to GFP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

   Insert

   GFP-MIOX
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL10

   Available since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTEF1-UDH

    Plasmid

    #166679

    Purpose

    Yeast integrative plasmid for expressing P. syringae uronate dehydrogenase, with TEF1 promoter. Contains leucine auxotrophic marker (K. lactis LEU2).

    Depositor

    Claudia Vickers

    Article

    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

    Insert

    UDH
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. wtVP1 only

    Plasmid

    #166673

    Purpose

    Yeast integrative plasmid for expressing wild-type Murine polyomavirus VP1, with GAL1 promoter. Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

    Insert

    Wild-type Murine polyomavirus VP1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28a-Hook3 Q147A, M151A, I154A

    Plasmid

    #74616

    Purpose

    Expresses a triple mutant truncation of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-239, Q147A, M151A, I154A (HOOK3 Human)
    

    Use

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    Q147A, M151A, I154A

    Promoter

    T7

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28a-Hook3 D102A, E108A

    Plasmid

    #74618

    Purpose

    Expresses a double mutant truncation of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-239, D102A, E108A (HOOK3 Human)
    

    Use

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    D102A, E108A

    Promoter

    T7

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28a-Hook3 N68A, W69A, K77A

    Plasmid

    #74619

    Purpose

    Expresses a triple mutant truncation of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-239, N68A, W69A, K77A (HOOK3 Human)
    

    Use

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pYZ033

    Plasmid

    #98404

    Purpose

    Entry vector for S. pombe CRISPR-Cas9 system. The gRNA can be integrated easily through Gibson Assembly with the plasmid backbone digested by Not1

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPW3758 : CMVd3-HsIRE1-HaloTag

    Plasmid

    #185680

    Purpose

    Low-level transient expression of full-length HsIRE1 with a C-terminal HaloTag.

    Depositor

    Peter Walter

    Article

    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

    Insert

    ERN1 (ERN1 Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MYC-hTRAK2

    Plasmid

    #225142

    Purpose

    Expresses MYC-tagged human TRAK2

    Depositor

    Thomas Schwarz

    Article

    Davis et al Life Sci Alliance. 2022 Oct 27;6(1):e202

    Insert

    TRAK2 (TRAK2 Human)
    

    Use

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pETSUMO2_Vitiosangium-bGSDM-thrombin

    Plasmid

    #222099

    Purpose

    For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Vitiosangium species with a thrombin protease cleavage site for controlled activation.

    Depositor

    Philip Kranzusch

    Article

    Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:

    Insert

    Vitiosangium-bGSDM-thrombin ORF
    

    Use

    Tags

    6xHis-SUMO2

    Expression

    Bacterial

    Mutation

    N-terminal methionine deleted and replaced by sin…

    Promoter

    T7

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pETSUMO2_Nostoc-bGSDM-WELQ

    Plasmid

    #222100

    Purpose

    For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Nostoc species with a WELQut protease cleavage site for controlled activation.

    Depositor

    Philip Kranzusch

    Article

    Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:

    Insert

    Nostoc-bGSDM-WELQ ORF
    

    Use

    Tags

    6xHis-SUMO2

    Expression

    Bacterial

    Mutation

    N-terminal methionine deleted and replaced by sin…

    Promoter

    T7

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBTK301

    Plasmid

    #110593

    Purpose

    BTK Type 6-7 terminator (replaces Type 6 and Type 7 parts)

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    Bba_B0015 Terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge